Residual K562 feeder cell Detection Kit
Residual K562 feeder cell Detection Kit
✓ Ultra-Sensitive Detection: Detects residual K562 feeder cells at levels as low as 0.05% (10 copies/μL), ensuring compliance with FDA guidelines for cell therapy safety.
✓ RT-PCR-Based Specificity: Utilizes optimized primers/probes targeting K562-specific sequences via RT-PCR, validated for specificity, accuracy, and precision per regulatory requirements.
✓ Wide Dynamic Range: Linear detection range of 10–1×106 copies/μL, covering trace residuals to high-concentration samples without dilution.
This kit utilizes RT-PCR with specifically designed target sites for the detection of residual K562 feeder cells. K562 cells engineered via gene editing are widely studied and utilized for their excellent proliferative properties. Research has shown that using gene-edited K562 cells as feeder cells can enhance NK cell expansion efficiency by tens to tens of thousands of times. Although K562 cells are irradiated and easily eliminated by NK cells, it is essential to ensure that residual feeder cells are minimized in the final product to ensure patient safety.
General Workflow
1.Preparation
Kit Components: Thaw all reagents (except primers/probes) at 4°C, then vortex and briefly centrifuge (6000 rpm, 5 sec).
Safety: Wear PPE (lab coat, gloves, mask) and work in a clean area to avoid contamination.
Equipment Setup:
Ensure qPCR instrument (e.g., ABI 7500, Bio-Rad CFX96) is calibrated with FAM/VIC channels.
Prepare RNA extraction and reverse transcription reagents.
2. Sample Pre-Treatment
RNA Extraction:
Isolate total RNA from 1×106–5×106 cells using a commercial RNA extraction kit.
Reverse Transcription:
Convert RNA to cDNA using gDNA removal and RT reagents (not included in kit).
3. PCR Reaction Mix Preparation
Standard Curve Dilution:
Serially dilute the K562 Quantitative Standard (1×108 copies/μL) with DNA Diluent to generate 6 points (10–1×106 copies/μL).
Example:
Master Mix Assembly (per reaction):
*Omit ROX if the instrument does not require it (e.g., Roche LightCycler).
Plate Setup:
Load 15μL Master Mix + 5μL sample/standard per well (triplicates for standards/samples). Include NTC (DNA Diluent).
Shipping Information
We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.
Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.
Important Information
Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.
Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.
Shipping Issues: If you find that your package has not been delivered within the specified time; the tracking information shows that the package has been delivered but you have not received it; or your package includes missing or incorrect items or other logistics issues, please contact our customer service within 7 days of the payment date so that we can address these issues promptly.
Shipping Restrictions
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Package Pickup Policy
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Note:As our product falls under the special category, returns and refunds are not accepted.
