Isidingo se-E.Coli Residial DNA Ukuhlola
Emasimini afana neseli nokwelashwa kwe-gene, ama-plasmid angamagciwane akhulisiwe futhi avulwe kusetshenziswa u-E. Coli njengomgcini. I-plasmid ekhulisiwe idinga ukubhekana nokulawulwa kwekhwalithi ye-e.coli esele ye-DNA ngaphambi kokuthi isetshenziselwe ukuthelela amaseli angama-293 bese iphakethe leli gciwane. Ngakho-ke, ukutholwa kwe-E.COLI Insary DNA kubalulekile.
Izidingo Zokulawula Nezindlela Zokuhlola
Imihlahlandlela yamanje ye-WNE NE-US FDA encoma imikhiqizo eqediwe kwimikhiqizo eqediwe ukuze ingabi ngaphezu kwe-10 ng / ejenti, futhi i-US FDA futhi iphinde ithi i-DNA eyinsalela kumaseli we-biologics akufanele ibe ngaphezulu kwe-100 PG / ejenti. Izimiso ezijwayelekile ze-European Pharmacopoeria zichaza ukuthi umkhawulo we-DNA oyinsalela emikhiqizweni yemvelo akufanele ube mkhulu kune-10 ng / umthamo ojwayelekile, kepha umkhawulo we-DNA osalelayo, futhi lowo emgomeni we-hepatitis B akufanele ube phezulu kuno I-10 PG / umthamo. Uhlelo lwe-2020 lwe-Chinese Pharmacopoeia, lube yingxenye ye-III, lubeka izinsalela ze-DNA emalungiselelweni ezinto eziphilayo ezikhiqizwa kwi-matrix yamaselula akufanele idlule ku-matrix yamaselula akufanele idlule kuma-matrix ayi-100 / fungal
Ngaphezu kwalokho, ngezindlela zokuzimisela okusasele kwe-DNA ye-DNA, i-National Pharmacopoeias inikeza nezincomo zokuholwa. I-U.S. Phoracopoeia 2017 Edition ye-USP40 2017 Ukuhlinzekwa Okujwayelekile kwe-1130 kuchaza izindlela ezintathu zokunqunywa kwezinsalela ze-DNA ezihlangene, okuyindlela ye-DNA Propetal hcry, indlela yangempela ye-PCR. I-European Pharmacopoeia iphakamisa ngokoqobo - isikhathi se-PCR ne-Immunoeenzyzyzymymatic indlela, izindlela ezimbili zokuhlaziya ezibucayi ze-Pount Personaal Remidapl DNA. I-Chinese Pharmacopoeia Imithetho emithathu ye-320 IMITHETHO EMI-3 Jikelele 3407 nayo ibeka ukuthi izindlela zokutholwa kwe-Cell DNA Residiue Readidization yi-DNA Prope
Phakathi kwazo, indlela ye-QPCR inokuzwela okuphezulu kakhulu, ukucacisa okuphezulu kakhulu nokunemba, okunganikezwa izindlela ezithembekile zokutholwa komkhakha we-biopharmace kumikhiqizo eqediwe, futhi manje sekuyindlela ekhethwayo yokutholwa kwabakhiqizi babo be-biologics.
Imininingwane mayelana nemikhiqizo ye-BlueKit
Izici zomkhiqizo
Ukutholwa okusheshayo, ukucaciswa okuqinile, ukusebenza okuthembekile, umkhawulo ophansi wokutholwa ungafinyelela ezingeni "FG".
Amapharamitha Womkhiqizo
- Uhla lokutholwa: 3.00 × 101 ~ 3.00 × 105FG / μL
- Umkhawulo wokulinganisa:00 × 101FG / μL
- Umkhawulo wokutholwa:00 FG / μL
- Ukucacisa: CV% ≤ 15%.
Izinkinga Zokutholwa Okujwayelekile kanye Nezinyathelo Zokuphepha
1. Le kit ingeyokusetshenziswa kocwaningo lwe-vitro kuphela, hhayi ukuxilongwa emtholampilo.
2. Ikhithi kufanele isetshenziswe esikhathini sokuvunyelwa.
3. Zonke izinto ezikukhithi kunconyelwe ukuncibilika endaweni ephansi - Imvelo yokushisa bese isetshenziswa.
4. Ukuthobela kuphela imiyalo yendlela yokusebenza, konke ukusetshenziswa kwekhithi esekela ama-reagents ukuqinisekisa umphumela omuhle kakhulu wokutholwa.
5. Naka izinyathelo ezihlukile zesampula ngendlela efike ngesikhathi ukuze ungene esikhundleni sethiphu, ukugwema isiphambano - ukungcola, gwema ukuvula isembozo isikhathi eside.
I-6. Imiphumela yokugcina yokuhlola ingaba nomthelela omkhulu ekusebenzeni kwama-reagents, izindlela zokusebenza kwe-opharetha kanye nemvelo yokuhlola.
Ukubonisana Komkhiqizo
Ucingo: +86 - 18013115357
I-imeyili:info@hillgene.com
Isikhathi Sokuthumela: 2024 - 01 - 11 10:31:30
