Imfuneko ye-E.Coli yovavanyo lwe-DNA ye-DNA
Kwimimandla efana neseli kunye nonyango lwe-gene, iplasmids yentsholongwane yenziwa i-wation kwaye yahlulwa isebenzisa i-E. Coli njengenginginya. IPlasmid ye-Amlmid kufuneka ithathe inxaxheba kwi-E.Coli I-DNA esemgangathweni ye-DNA ngaphambi kokuba isetyenziselwe ukosuleleka iiseli ezingama-293 kunye nephakheji intsholongwane. Ke ngoko, ukufunyanwa kwe-E.Coli I-DNA yentsalela ibalulekile.
Iimfuno zolawulo kunye neendlela zokuvavanya
Okwangoku izikhokelo ze-FDA kunye ne-US DE Cena i-DNA yentsalela kwiimveliso ezigqityiweyo ukuba zingabi ngaphezu kwe-10 nge-10 NG / iarhente, kwaye i-FNA ye-US ikwatsho ukuba inkulu kune-100 pg / iarhente. Imigaqo ngokubanzi ye-European Pharmacopoeia ichaza ukuba umda we-DNA ye-DNA ye-Biological kwi-HG / i-DNA ye-HG / DOSE, kwaye i-DNA ye-HEATATARY STATITO YOKUGQIBELA I-HEATITIIS ASUTRY BFATITIC AKUFUNI KUPHELA kune-10 pg / idosi. Ushicilelo lwe-2020 lwe-pharmacopoeia yaseTshayina, iCandelo III, icacisa ukuba amalungiselelo e-DNA ngendalo aveliswe kwi-100 pg ye-i-Itrix engafanelekanga ukuba ayidlule kwi-10 godlix engapheliyo kwi-10 NG / idosi.
Ukongeza, kwiindlela zokuzimisela kwe-DNAgenesnous DNA, i-pharmacopoeiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiaiais zikwanika neengcebiso ezikhokelayo. I-U.S. pharmacoopoeia 2017 i-WCED i-USP40 ye-USP35 ichaza iindlela ezintathu zokumiselwa kwe-DNAgeneus ye-DNAgenous, eyindlela ye-DNA ye-PNNAP, indlela ye-PNA ye-PCR. I-pharmacopoeia ye-European iphakamisa inyani - Ubungakanani bexesha le-PCR kunye nendlela ye-gonoenzymatic, iindlela ezimbini zohlalutyo lwe-DNA. I-Chinese Pharmacopoeia 2020 Inguqulelo yeMithetho emithathu Jikelele 3407 ikwachaza ukuba iindlela zokuchonga i-DNA I-DNA I-DNA Sybridation, i-Pluorescence Studing kunye ne-PRCR.
Phakathi kwazo, indlela ye-QPCR inobuntu obugqithisileyo, ukulandelelana ngokuchanekileyo, okunokubonelela ngeendlela ezinokufunyanwa kophando lwemveliso yophando, kwaye ngoku ingaba yindlela yokufunyanwa kwebhayoloji nganye.
Ulwazi malunga neemveliso zeBluekit
Iimpawu zeMveliso
Ukufunyanwa ngokukhawuleza, ubuchwephesha obuqinileyo, intsebenzo ethembekileyo, umda wokuqaphela ophantsi unokufikelela kwinqanaba "FG".
I-parameter yemveliso
- Uluhlu lokuBoleka: I-3.00 × 101 ~ 3.00 × 105fg / μl
- Umda wokuQala:00 × 101fg / μl
- Umda wokufumanisa:00 fg / μl
- Ukuchaneka: cv% ≤ 15%.
Iingxaki zokufumana eziqhelekileyo kunye nezilumkiso
1. Le kit ilungiselelwe ukusetyenziswa vitro kusetyenziswa kuphela, kungekuko ukuxilongwa kweklinikhi.
2. Ikhithi kufuneka isetyenziswe kwixesha lokuqinisekiswa.
3. Zonke izinto ezikwikhiti ziyacetyiswa ukuba zinyibilike kwindalo esezantsi - ubushushu kwaye emva koko basebenzise.
4. Ngokuthobeka ngokungqongqo ngokungqinelana nemiyalelo yendlela yokusebenza, konke ukusetyenziswa kwe-kit exhasa ii-regeents ukuqinisekisa ukuba eyona nto ifunyenweyo.
5. Nikela ingqalelo kumanyathelo esandi-sandi ngexesha lokutshintsha inqaku, ukunqanda ukuwela - ukungcolisa, kuphephe ukuvula isiciko ixesha elide.
I-6. Iziphumo zokugqibela zovavanyo zinokuba zichaphazeleke kakhulu ngokusebenza kwempumelelo, iindlela zokusebenza zomsebenzi kunye nendawo yovavanyo.
Ukubonisana kwemveliso
IFONI: +86 - 18013115357
I-imeyile:Ifayile@hillgene.com
IXESHA LOKUQALA: 2024 - I-01 - 11 10: 31:30:30
