IiNkonzo zeDDM kwiPlasmids - Ibakala le-Indili
Iinkonzo
| IiNkonzo zeDDMIDs zePlasmids | ||||
| Iintlobo | Iinkonzo | |||
| Ibakala le-ID | 1 | Ngokuzimeleyo ezimeleyo ezine - Inkqubo yePlasmid |
● Isizukulwana sesithathu - Inkqubo yePlasmid ● I-Kanamycin - Ukuxhathisa uhlobo ● Ukunika ilayisensi, ukuba iyafuneka |
● Ukulandela imigangatho yokungeniswa kwi-China kwaye nathi ● Izele yindawo yokusebenzela ● Indawo eyahlukeneyo yokwenza iibhanki zeseli ● Iworkshop ezahluliweyo ngaphakathi kwezi - ezinobungozi kunye nezingaphantsi ● Inkqubo yolawulo lwe-GP |
| 2 | I-GMP yokuKholoswa kweBhanki ye-bactery |
● Ukukhetha ii-antibodies ze-monoclon ● Inani elijolise kwiibhanki zeseli ukuba zenziwe ● Isifundo sozinzo zebhanki |
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| 3 | Inkqubo kunye noPhuculo lweNdlela yoVavanyo |
● Ukulandela iimfuno zeprojekthi (phantsi kotshintsho oluqhelekileyo) |
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| 4 | Ukuveliswa kwePlasmids |
● Imveliso yemveliso: 10 mg ~ 1 g (phantsi kotshintsho olufanelekileyo) ● Umthamo owodwa: 3 ~ 30 l (phantsi kotshintsho olufanelekileyo) ● Indlela yokucoca: Isithathu - Indlela yenyathelo / indlela yenyathelo |
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| 5 | Uvavanyo lwePlasmid |
● Ukucoceka (i-HPPC) ● Ukuvavanywa kwe-E.Coli DNA ● Ukuvavanywa kwe-e.coli HCP ● Intsalela yokuvavanywa kwe-E.Coli RNA ● Intsalela yovavanyo lweentsholongwane ● Ukungena ● I-Mycoplasma ● I-Endotoxin |
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| 6 | Indlela yokuqinisekisa |
● Ngokuqhelekileyo ● Ukuchaneka ● Ukuchaneka ● umgca kunye nodidi ● Rhoqo |
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| 7 | Isifundo sokuzinza |
● Ixesha elide - Uzinzo lwekota ● Uzinzo olukhawulezileyo ● Uvavanyo loxinzelelo |
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Izibonelelo
| Izibonelelo zenkqubo yethu yePlasmid:
• Isine esizimeleyo esizimeleyo - Inkqubo yePlasmid nge-kanamycin - Ukuchasana ne-gene Inkqubo enokwazi ukusebenzela Ukulandelelana kwePlasmid kuyalungiswa, kuyahambelana neemfuno, kwaye kufanelekile • Amava abalulekileyo ekungeneni kwempumelelo • Imoto - T Isampulu zeselfowuni zosetyenziso lweklinikhi zinemveliso kwaye zisetyenziswa • I-2 - Iiposti ezi-5 ziPhakamileyo emva kokusebenzisa inkqubo yethu yePlasmid evela ekuthelekiseni iiprojekthi ezininzi |
IINGXAKI ZOBOMI BAKHO: Mahala kumayeza okubulala iintsholongwane kuyo yonke inkqubo yokuphuhlisa • Imveliso yePlasmid kunye nokuBekwa kweBhanki kwiindibano zocweyo ezahlukeneyo Ukuzahlula-hlula okuphezulu phakathi kweendawo ezingezizo - iindawo ezinobutshelweyo • Ukusasaza iimveliso zokugqibela usebenzisa i-isolator • I-Dessiers egqityiweyo ye-CTD yokupakisha i-plasmid (ye-Lentiviviral Vector), ukunciphisa ixesha lokungenisa eli-3 - Iinyanga ezi-4, kunye ne-Inds yeemveliso ezimbalwa zinikezelwe kwisigaba seKlinikhi |
Inkqubo yokwenziwa kwemveliso
Ulawulo lwemeko
| Into yovavanyo | Indlela yovavanyo | |
| Imbonakalo | Uvavanyo olubonakalayo | |
| Isazisi | Isazisi 1 | Imephu yokuthintela |
| Ukuchonga 2 | Ukulandelelana kwesangel | |
| Vavanyo | pH | Indlela 0631 ye-CHP 2020 |
| Ubunyulu | Intsebenzo ephezulu ye-chromatography (i-HPPC) | |
| Intsalela ye-E.Coli ibamba iproteyini yeseli | Elisa | |
| Intsalela ye-E.Coli DNA | Q - I-PCR | |
| Intsalela ye-E.Coli RNA | Q - I-PCR | |
|
I-Avibiotics |
Elisa | |
| Endotoxin | Indlela ye-1143 ye-CHP 2020 | |
| Ukuncwina | Indlela i-1101 ye-chp 2020 | |
| Ukuziqhelanisa nokuzihlaziya | I-DNA uxinzelelo | Indlela 0401 ye-CHP 2020 |
Ixesha leprojekthi
Isicwangciso soLawulo lweProjekthi
Iqela leProjekthi yase-Rillgene, equka izazinzulu zeprojekthi, abaphathi beeprojekthi, iingcali zeProjekthi zePREP, ziya kwenza imizamo yokuqinisekisa ukusebenza kakuhle nokuvakalayo kweprojekthi nganye ye-GMP.
