Litšebeletso tsa CDM bakeng sa li-fictors tsa lentiviral - Kena
Litšebeletso
| Kena | 1 | E itsebahalo e 'ngoe e ntlafalitsoeng - Sistimi ea Plasmid |
● Moloko oa boraro oa 'me mane - Sistimi ea Plasmid ● Kanamycin - Gened Gene ● Ho fa laesense, haeba ho hlokahala |
● Ho latela melao ea ho ikokobelletsa Chaena le rona ● Kakaretso - Sehlopha sa GPP ● Karolo ea morao-rao ea ho theha libankeng tsa sele ● Lithunya tse arohaneng kahare ho moo ho sa sebetseng hantle le libaka tse sa lokang ● Sistimi ea boleng ba Lipallo tsa GPP |
| 2 | Pōpo ea GMP Cell Bank |
● Palo e sa rateheng ea libanka tsa lisele e thehoa ● Ho ithuta Bank sa botsitso |
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| 3 | Ts'ebetso le nts'etsopele ea tlhahlobo ea liteko |
● Ho latela litlhoko tsa merero (ho latela liphetoho tse tloaelehileng) |
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| 4 | Ho etsa lihlahisoa tsa li-fictors tsa lesole |
● Ts'ebetso ea Biorector: ● Sekhahla sa Product: 2 ~ 30 l (ho latela liphetoho tse tloaelehileng) |
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| 5 | Teko ea li-ficto tsa lepotlali |
● Setaimi sa 'mele ● Setsi se sa sebetseng ● Ts'ebetso ea Ts'ebetso ● Nako ea 293t ● Khanya ea lifofane tsa 29T tsa nako e telele ● Teko e masene ea DNA ea DNA ● Teko ea Benzona e setseng ● E1A / SV40 ● Teko ea masala a pherekano ● Dana Fragment ● Exsenius Viakines ● Borteba ● Mycoplasma ● Bofetoi |
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| 6 | Netefatso ea Mokhoa |
● Bohlokoa ● Bohlokoa ● Ho nepahala ● Toarity le bongata ● Lota |
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| 7 | Thutong ea botsitso |
● Nakoana - ho tsitsa ● Ho tsitsa e potlakileng ● Litlhahlobo tsa khatello ea maikutlo |
Melemo
| Melemo ea ho sebelisa sethala sa rona bakeng sa serum - Ho thibela mahala mahala ha lijana tsa lepotlatlatla.
• Ho se na baahi - likarolo tse tsoang ho pholletsa le ts'ebetso • tlhahiso e tsitsitseng ka mokhoa o hlakileng oa li-ficto tsa lesole • Ho sebelisa setshwao se le seng sa biorenactor e 50 e lahlehileng • Pōpo ea banka ea sele e libakeng tse arohaneng • Ho amohela lihlahisoa tsa ho qetela u sebelisa karohano e mpe • Sistimi ea Khokahano ea Khokahano ea Konopo ea Koloi ea Koloi ea Koloi ea Koloi, e nang le ts'ebetso e phahameng ea ts'oaetso • Litsenyehelo tse tlase tsa tlhahiso le liteko tsa tlhahlobo (ha ho na litlhoko tsa tlhahlobo ea BSA le li-enzymes tsa ho paka) • Likarolo tse 'maloa tsa katleho ho Nmpa ea li-ficto tsa lentiviral bakeng sa koloi ea koloi - |
Ts'ebetso ea Tlhahiso
Taolo ea Boleng
| Sehlahisoa | Ntho ea liteko | Mokhoa oa liteko |
| Mokotula Motulo | Tlhahlobo e ntle ea vaerase | Metho 3302 ea chp 2020 |
| TLHOKOMELISO - LITLHAKISO TSE BOTSOANG | Matšoao a setso a bonono | |
| Ntho e sebelisang lithethefatsi / sehlahisoa se felileng | Ponahalo | Tlhahlobo e bonahalang |
| Thel | Mokhoa oa 1101 oa chp 2020 | |
| Mycoplasma |
Metho 3301 ea chp 2020 |
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| pH | Mokhoa oa 0631 oa chp 2020 | |
| Osmollality | Mokhoa oa 0632 oa chp 2020 | |
| Boitsebiso ba Gener Gevent Gevent " | E lekantsoe | |
| Sepone sa seleng se tsitsitseng sa selefounu | Elisa | |
| 'Mele oa' mele (p24) | Elisa | |
| Ts'ebetso ea Sebetsa | Phallo ea cytometry | |
| Endotoxin | Mokhoa oa 1143 oa chp 2020 | |
| Benzonase ea Mafunyane | Elisa | |
| DNA e phahameng ea sele Dna | Q - pcr | |
| Phetoho ea Gener ea E1A ea E1A | CO - Mokhoa oa setso | |
| Phetisetso ea SV40 ea mofuta oa SV40 Gener | CO - Mokhoa oa setso |
Morero oa Time
Morero oa taolo ea morero
Sehlopha sa Tsamaiso ea Morero oa Hillgene, o nang le bo-ramahlale ba bararo, batsamaisi ba Tii, morero oa QMP, ba tla etsa boiteko ba ho netefatsa ts'ebetso e boreleli le ea molumo oa morero o mong le o mong oa GP.
