Ibikenewe kuri e.coli ibizamini bya ADN
Mu mirima nka selile na gene, ibyapa bya virusi byakorojwe kandi bisebya gukoresha E. Coli nkabakiriye. Plasmid Yongerewe ikeneye kunyuramo E.COLI kugenzura ubuziranenge bwa ADN bwa ADN mbere yuko bukoreshwa muri selile 293 hanyuma bapakira virusi. Kubwibyo, gutahura e.coli ADN isigaye ni ngombwa.
Ibisabwa kugenzura no kugerageza uburyo bwo kugerageza
Ubuyobozi bugezweho na FDA muri Amerika busaba ADN ibisigazwa bisigaye kugirango tutarenze 10 NG / Agent yo muri Amerika ivuga ko ingirangingo zisigaye zakazi zitemewe n'amategeko zidakwiye kurenga 100 PG / umukozi. Amahame rusange ya Pharman Uburayi ateganya ko imipaka ya ADN ibinyabuzima idakwiye kurenza urugero, urugero rwa ADN zisigaye ku rukingo rw'ibice, kandi ko mu rukingo rwo kurwanya Hepatite B ntigomba kuba hejuru kurenza 10 pg / igipimo. Igitabo cya 20220 cya Pharmacopoeia Igishinwa, Igice cya III, giteganya ko igisigara cya ADN mubitegura ibinyabuzima byakorewe kuri matrix 100
Byongeye kandi, ku buryo bw'icyemezo cya Exogieus ADN gisise, Pharmacoppoeas yigihugu nayo itanga ibyifuzo. Igitabo cya U.S. Pharmacopoeia wo mu Burayi itanga nyazo - Igihe ntarengwa PCR hamwe nuburyo bwo gusesengura, uburyo bwo gusesengura bukabije bwo guhuza Astle yakiriye Apple. Igishinwa cya Pharmacopoeia 2020) Amategeko atatu rusange 3407 kandi ateganya ko uburyo bwo kumenya Akagari ADN Pro Rebblisation, Fluorescence Stain na PCR.
Muri bo, uburyo bwa QPCR bufite ubushishozi bukabije, bukurikiranye umwihariko kandi busobanutse neza kandi bushobora gutanga uburyo bwizewe bwo kumenya inganda za biopharmace mu buryo bwuzuye, kandi ubu bwabaye uburyo bwo kumenya neza buri buzima bwa biologique.
Amakuru yerekeye ibicuruzwa by Bluekit
Ibicuruzwa biranga ibicuruzwa
Kumenya byihuse, umwihariko, imikorere yizewe, imipaka yo guhitamo hasi irashobora kugera kurwego rwa "FG".
Ibipimo by'ibicuruzwa
- Kumenya Urwego: 3.00 × 101 ~ 3.00 × 105fg / μl
- Imipaka yo kwinjiza:00 × 101fg / μl
- Imipaka ntarengwa:00 fg / μl
- Precision: CV% ≤ 15%.
Ibibazo bisanzwe no kwirinda ingamba
1. Ibi bikoresho biri mubushakashatsi bwa vitro gusa, ntabwo ari ugusuzuma amavuriro.
2. Igikoresho kigomba gukoreshwa mugihe cyemewe.
3. Ibigize byose mubikoresho birasabwa gushonga mu bushyuhe buriho - ubushyuhe hanyuma bukoreshwa.
4. Mubyukuri byubahirije amabwiriza yuburyo bukoreshwa, gukoresha ibikoresho byose bishyigikira reagents kugirango hamenyekane neza ingaruka nziza.
5. Witondere intambwe zitandukanye zitoranya mugihe gikwiye kugirango usimbuze inama, kugirango wirinde umusaraba - kwanduza, irinde gufungura umupfundikizo igihe kirekire.
6. Ibisubizo byikizamini byanyuma birashobora kubafungurwa cyane nibikorwa byisubiramo, uburyo bwo gukora ibikorwa nibidukikije.
Kugisha inama ibicuruzwa
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Igihe cyagenwe: 2024 - 01 - 11:31:30
