Kufunikira kwa E.Coli Otsalira Kuyesa kwa DNA
Mu minda monga foni ndi gene mankhwala, ma plasmid ma plasmid amakula ndi kupanikizika pogwiritsa ntchito E. Coli monga mwininyumbayo. The Amplifid Plasmid imafunikira kuwongolera E.Coli Reformally DNA yabwino isanagwiritsidwe ntchito kutchinjiriza maselo 23 ndikupanga kachilomboka. Chifukwa chake, kupezeka kwa E.Coli kotsalira kwa DNA ndikofunikira.
Zofunikira zowongolera ndi njira zoyesera
Pamakono omwe ndi malangizo a US FDA Purces amalimbikitsa otsalira pazinthu zopitilira 10 z Mfundo zambiri za Europe wa MarU waku Europe PG / Mlingo. Magazi 2022 Kutulutsa kwa Memacopoeia, gawo la III, ndikupanga kuti kutsalira kwa DNA Kukonzekera kwa ma cellolar sikuyenera kupitirira 100 pg / mlingo wa matrix sayenera kupitirira 10 ng / mlingo.
Kuphatikiza apo, pamayendedwe a Exo nationa kutsimikiza, mayiko a National Pharmacoeas amaperekanso malingaliro owongolera. The U.S. Makina a USP400 - NFP35 imafotokoza njira zitatu zotsalira za DNA Sporsication, njira yolowera - Njira ya PCR Quiting. European pharmacopoeia imalikulu - Nthawi yochulukirapo PCR ndi ImmunonzyMozym njira yowunikira, njira ziwiri zowunikira zotsalazo za cell. Mtundu wa Demacopopoeia 2020 wa malamulo a General 3407 amafotokozanso kuti njira zotsalira za cent dna prober ndi DNA Spobertion, mapangidwe a flurescence.
Mwa iwo, njira ya QPCR ili ndi chidwi chachikulu, kutsatira mawonekedwe ndi kulondola kwa mabizinesi a biophormaumical pofufuza zinthu zomaliza, ndipo tsopano yakhala njira yodziwika ya opanga zopanda pake.
Zambiri zokhudzana ndi zinthu zamtambo
Mawonekedwe a malonda
Kuzindikira mwamphamvu, kulimba mtima, ntchito yodalirika, malire otsika kwambiri amatha kufikira gawo la "fg".
Magawo ogulitsa
- Kuzindikira: 3.00 × 101 ~ 3.00 × 105fg / μl
- Malire a kuchuluka:00 × 101fg / μl
- DZIWANI IZI:00 FG / μl
- Molondola: CV% ≤ 15%.
Mavuto Odziwika Komanso Kusamala
1. Izi zili mu kafukufuku wogwiritsira ntchito vitro pokhapokha, osati chifukwa cha matenda.
2. Kit iyenera kugwiritsidwa ntchito mkati mwa nthawi yovomerezeka.
3. Zinthu zonse zomwe zili mu kit zikulimbikitsidwa kusungunuka mu otsika - kutentha malo kenako kugwiritsidwa ntchito.
4. Pulogalamu mosamalitsa ndi malangizo a njira yogwiritsira ntchito, kugwiritsa ntchito zonse zothandizira ma reigent kuti zitsimikizire kuti mwazindikira bwino.
5. Samalani ndi njira zosiyanasiyana zosinthira munthawi yake kuti musinthe nsonga, kupewa kuwoloka - kuipitsidwa, kupewa kutsegula chivundikiro kwa nthawi yayitali.
6. Zotsatira zomaliza zitha kukhudzidwa kwambiri ndi kugwira ntchito kwa ma reagents, njira zogwirira ntchito ndi malo oyeserera.
Kufunsa kwa Ntchito
Telefoni: +86 - 180131115357
Imelo:info@hillgene.com
Post Nthawi: 2024 - 01 - 11:31:30
