Ntchito za CDMO kwa vetikir - Ind

Lentivirus, subype wa retrovirus, imatha kuphatikiza majini a chandamale mu masenti omwe ali pachiwopsezo, ndipo nthawi zambiri amagwiritsidwa ntchito ngati veral verring encriner urlering. Ndi kutuluka kwa makompyuta othandizira ma cell, zomwe zimapangitsa kuti ma vectore a Lentivil a Lentiviral akuwonjezekanso chaka chilichonse. Mapiri ali ndi mwayi pakukonzekera kwa CDMO yophatikizira za ma cell othandizira ma cell roral rarum -

Ntchito

Ind	1	Palokha adapanga anayi - Pulogalamu ya Plasmid	● Mbadwo wachitatu 0 - Plasmid Dongosolo ● Kanamycin - kukana Gene ● Kupatsa chilolezo, ngati pakufunika	● Kutsatira miyezo yogonjera ku China ndi ife ● Kudzaza - gmp ● Malo osiyanitsa mabanki ● Zokanikirana zopatukana mkati mwa malo osabala komanso osabala ● GMP YOPHUNZITSIRA MALO OGULITSIRA
	2	Kulengedwa kwa BPM Bank	● Chiwerengero chamiyala ya ma cell kuti chilengedwe ● Phunziro lokhazikika la foni
	3	Njira ndi kuyesa kwa njira	● Kutsatira zofunikira pokonzekera polojekiti (malinga ndi kusintha kwa zosintha)
	4	GMP wopanga ma vetikiyi a lentivisiral	● Bireactor: 5 ~ 50 l kutayika njira ya bioreactor (malinga ndi kusintha kwachilengedwe) ● Gawo lopanga: 2 ~ 30 l (malinga ndi kusintha kwachilengedwe)
	5	Kuyesa kwa ma vetivisi a Lentivil	● MATT NKHANI ● Chithandizo cha Chakhumi ● Chair ● Kuyeserera kwa 293T kuyezetsa kwa maselo ● Kuyeserera kotsalira kwa 293T ● Kuyeserera kokhazikika kwa DNA ● Kuyesa kotsalira kwa Benzonase ● e1a / sv40 ● Kuyesa kotsalira kwa Plasmid ● Kukula kwa DNA ● Zivomezi za exunous ● Kusasankhidwa ● Mycoplasma ● Endotoxin
	6	Njira yotsimikizika	● Kutengera ● Kulondola ● Kulondola ● Chilotinga komanso mtundu ● LoD
	7	Kuphunzira kafukufuku	● Kutalika kwa - Kukhazikika kwa Nthawi ● Kusunthika mwachangu ● Kuyeserera kwa nkhawa

* Dziwani: Timapereka kusintha kosinthika ndi kusinthasintha kwa ntchito, kuphatikiza koma osangokhala pa ntchito.

Ubwino

Ubwino Wosintha nsanja yathu ya seramu - Kuyimitsidwa Kwaulere Kuyesedwa kwa Ma vectors a Lentivisi:

• wopanda nyama - zopangidwa ndi zomwe zimapangidwa munthawi yonseyi

• Kupanga molingana ndi ma vetivisi

Kugwiritsa ntchito chidebe chimodzi cha 50 l

• Cet Cet Bank mu Zolemba Zosakaniza

• Kuyika zinthu zomaliza pogwiritsa ntchito indolator yosalala

• Makina odzipereka a Lentivirus agalimoto - T maselo, okhala ndi mphamvu yayikulu

• Ndalama zochepa zopanga ndi ndalama zoyeserera (palibe zofunikira pakuyesa BSA ndi zotsalira pancreatic michere)

• Kutumiza kopambana komwe kumayenderana ndi mipata ya ventivisi ya lentivisi yagalimoto - T

Kupanga

Kuwongolera kwapadera

Chinthu	Chiyeso	Njira Yoyesera
Kukolola madzi	Kuipitsidwa kwa kachilombo kodetsa	Njira 3302 ya chip 2020
Kukolola madzi	Kubwezera - Lentitent Lentiirses	Njira yachizindikiro ya cell
Zogulitsa / Zomalizidwa	Kaonekedwe	Kuyang'ana Zowoneka
	Kusamutsa	Njira 1101 ya Chip 2020
	Mycoplasma	Njira 3301 ya Chip 2020
	pH	Njira 0631 ya Chip 2020
	Oswelality	Njira 0632 ya Chip 2020
	Chidziwitso cha Get Center	Kusazidza
	Zotsalira zotsalira za protein	Elia
	Ngwazi yakuthupi (P24)	Elia
	Zogwirira Chake	Kutulutsa cytometry
	Endottoxin	Njira 1143 ya Chip 2020
	Zotsalira benzonase	Elia
	Zotsalira za cell DNA	Q - PCR
	Kusamutsa kwa E1a Genes	CO - Njira Chikhalidwe
	Kutumiza kwa SV40	CO - Njira Chikhalidwe

* Dziwani: Mapiri okhazikitsidwa qc ofananira ndi nsanja zosiyana zaukadaulo, ndi njira za QC kuphatikiza koma osangokhala pamwamba pa zinthu.

Project Projekine

Dongosolo Loyang'anira Ntchito

Gulu la Phill Hillgene

Kutsitsi

Kuphatikiza njira za CDMO yagalimoto - T

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