CAR-T Cell Serum-Free Preparation Kit

CAR-T Cell Serum-Free Preparation Kit

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✔ Integrated magnetic beads yield >95% pure CD3+ cells (vs. 80-85% in Miltenyi kits)
✔ Ready-to-use lentivirus with pre-titrated MOI (saves QC time)
✔ Includes 21 CFR Part 11 documentation
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Overview
Protocols
Specifications
Shipping & Returns
Video Recording

Product Overview:
The Serum-free CAR-T Cell Manufacturing Kit comprises three integrated modules:


T Cell Activation & Expansion Module


T Cell Basal Medium: Provides essential nutrients for T cell culture


T Cell Activator: Delivers activation signals through optimized stimulatory molecules


T Cell Purification Module


Magnetic bead-based isolation system for high-purity T cell enrichment from leukapheresis products or PBMCs


CAR Gene Transduction Module


Ready-to-use CAR Lentivirus


Viral Transduction Enhancer (Viral E-hancer B): Increases CAR integration efficiency


Key Features:


Simplified workflow for serum-free CAR-T production


High expansion potential (>50-fold) with maintained viability (>90%)


Superior transduction efficiency (typically >60% CAR+)


Consistent T cell purity (>95% CD3+)


Applications

Generation of CAR-T cells from peripheral blood or cord blood

Process development: Accelerates timeline and reduces costs for early-stage CAR-T therapy development


Performance Specifications

Parameter

Specification

Expansion Fold

>50x

Cell Viability

>90%

Transduction Efficiency

>60% CAR+

T Cell Purity

>95% CD3+

Data Highlights:

The BlueKit® Serum-Free CAR-T Cell Manufacturing System integrates three core functional modules:

T Cell Isolation & Purification

T Cell Expansion & Culture

CD19 CAR Gene Transduction


Key Performance Data:

Consistently achieves >98% purity in isolated T cell populations (see Figure)

Maintains high viability (>95%) throughout the manufacturing process

Enables efficient CAR transduction (typical efficiency: 60-80%)

 


Process Performance:

The purified T cells (>98% purity) are subsequently activated using the T Cell Activator and expanded in optimized T Cell Basal Medium,

achieving:>100-fold expansion,>90% viability throughout the culture period


This expansion module maintains T cell fitness and functionality while supporting robust proliferation.

 





Performance Parameter Specification
NK Cell Purity (%) >90%
CD3+ Cell Residual (%) <1%
NK Cell Expansion Fold >5,000-fold
Cell Viability (%) >80%



Bluekitbio is committed to providing the best products in the experimental research industry.
 

Shipping Information

We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.

 

 Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.

 

 Important Information

Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.

 

Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.

 

Shipping Issues: If you find that your package has not been delivered within the specified time; the tracking information shows that the package has been delivered but you have not received it; or your package includes missing or incorrect items or other logistics issues, please contact our customer service within 7 days of the payment date so that we can address these issues promptly.

 

Order Tracking

Once your order is shipped, you will receive an email with a tracking number and a link to track your shipment.

 
You can also track your order directly on our website by logging into your account and viewing your order history.

 

Shipping Restrictions

Please fill in the street address in detail, not PO BOX or military address (APO). Otherwise, we would have to use EMS for delivery (it's slower than others, taking around 1-2 months or even longer).

 

Customs Duties and Taxes Policy

Please note that any customs duties, taxes, or import fees incurred during shipping are the responsibility of the buyer. These charges vary depending on the destination country and are determined by local customs authorities.

By purchasing from our website, you agree to pay any applicable duties or taxes associated with your order. We are not responsible for delays caused by customs clearance.

 

Package Pickup Policy

Once your order has arrived at the designated pickup point or delivery location, please ensure prompt collection. If the package is not picked up within the designated time, we will send a reminder via email or SMS. However, if the package is not collected within the specified period, and any loss or damage occurs as a result, the buyer will be held responsible. We kindly remind you to collect your package promptly to avoid any potential issues.

Note:As our product falls under the special category, returns and refunds are not accepted.

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