Quality control of q-PCR technology 

Quantitative Real-time PCR (PCR) is a method in which a fluorescent chemical is used to measure the total amount of product after each PCR cycle in DNA amplification. A method of quantitative analysis of a specific DNA sequence in a test sample by internal or external parameters. 

Real-time timePCR is a real-time detection of PCR  through fluorescence signal during PCR amplification. In the exponential period of PCR amplification, the Ct value of the template has a linear relationship with the initial copy number of the template, so it becomes the basis of quantification.