IiNkonzo zeDDM kwiPlasmids - Inqanaba leklinikhi

Imveliso yePlasids, inyathelo elibalulekileyo lemoto yemveliso - Imveliso yonyango lweselula, kubandakanya uthotho lweenkqubo ezinzima zokwenza imveliso, ukucocwa, kunye nohlalutyo. Njengoko izixhobo ezibalulekileyo zobunjineli bemfuza, i-bacterial plasmids ayinakusetyenziswa kuphela njengemveliso yokugqibela yemveliso ye-gene kunye nemveliso yeselula, kodwa njengeeVenkile zoNyango zoNyango, kwaye zisetyenziswa ngokungafanelekanga kwiimveliso ezininzi kunye neemveliso zonyango lweselula. Ngokuvela kwemveliso yonyango lweselula, iimfuno zentengiso zePlasmids ziyanda kunyaka ngamnye odlulayo. I-Hilgene ikhethekileyo ekuboneleleni ngezisombululo ze-CDM ezihlanganisiweyo zeemveliso zonyango zeselula, kwaye ke iqonga leeNkonzo zeDlueidi, kwaye ke, zinokubonelela ngeenkonzo eziphezulu zeplasmids kubaxumi abaneemfuno ezahlukeneyo.

Iinkonzo

IiNkonzo zeDDMIDs zePlasmids
Iintlobo			Iinkonzo
Inqanaba leklinikhi	1	Ukuveliswa kwePlasmids	● Imveliso yemveliso: 10 mg ~ 1 g (phantsi kotshintsho olufanelekileyo) ● Umthamo owodwa: 3 ~ 30 l (phantsi kotshintsho olufanelekileyo) ● Indlela yokucoca: Isithathu - Indlela yenyathelo / indlela yenyathelo	● Izele yindawo yokusebenzela ● Iworkshop ezahluliweyo ngaphakathi kwezi - ezinobungozi kunye nezingaphantsi ● Inkqubo yolawulo lwe-GP ● Isityalo esiqinisekisiweyo, indawo kunye nezixhobo ezihambelana neemfuno zeklinikhi
Inqanaba leklinikhi	2	Ukudluliselwa kwetekhnoloji	● Ukudluliselwa kwetekhnoloji ● Ukufumana ukuhanjiswa kwetekhnoloji	● Well - Isicwangciso esisekwe kwiTekhnoloji yeTekhnoloji ● Well - Isicwangciso esisekelweyo sokufumana itekhnoloji yokudluliselwa kwetekhnoloji ● Cwangcisa ukutshintshelwa kweetekhnoloji ezahlukeneyo kuzo zonke izigaba ezahlukeneyo

* Qaphela: I-Hilgene eseka iindlela ze-QC ezihambelana namaqonga etekhnoloji eyahlukileyo, kunye neendlela ze-QC kubandakanya kodwa zingapheleli kwizinto zezinto ezingaphezulu.

Izibonelelo

Izibonelelo zenkqubo yethu yePlasmid:

• Isine esizimeleyo esizimeleyo - Inkqubo yePlasmid nge-kanamycin - Ukuchasana ne-gene

Inkqubo enokwazi ukusebenzela

Ukulandelelana kwePlasmid kuyalungiswa, kuyahambelana neemfuno, kwaye kufanelekile

• Amava abalulekileyo ekungeneni kwempumelelo

• Imoto - T Isampulu zeselfowuni zosetyenziso lweklinikhi zinemveliso kwaye zisetyenziswa

• I-2 - Iiposti ezi-5 ziPhakamileyo emva kokusebenzisa inkqubo yethu yePlasmid evela ekuthelekiseni iiprojekthi ezininzi

IINGXAKI ZOBOMI BAKHO:

Mahala kumayeza okubulala iintsholongwane kuyo yonke inkqubo yokuphuhlisa

• Imveliso yePlasmid kunye nokuBekwa kweBhanki kwiindibano zocweyo ezahlukeneyo

Ukuzahlula-hlula okuphezulu phakathi kweendawo ezingezizo - iindawo ezinobutshelweyo

• Ukusasaza iimveliso zokugqibela usebenzisa i-isolator

• I-Dessiers egqityiweyo ye-CTD yokupakisha i-plasmid (ye-Lentiviviral Vector), ukunciphisa ixesha lokungenisa eli-3 - Iinyanga ezi-4, kunye ne-Inds yeemveliso ezimbalwa zinikezelwe kwisigaba seKlinikhi

Inkqubo yokwenziwa kwemveliso

Ulawulo lwemeko

Into yovavanyo		Indlela yovavanyo
Imbonakalo		Uvavanyo olubonakalayo
Isazisi	Isazisi 1	Imephu yokuthintela
Isazisi	Ukuchonga 2	Ukulandelelana kwesangel
Vavanyo	pH	Indlela 0631 ye-CHP 2020
	Ubunyulu	Intsebenzo ephezulu ye-chromatography (i-HPPC)
	Intsalela ye-E.Coli ibamba iproteyini yeseli	Elisa
	Intsalela ye-E.Coli DNA	Q - I-PCR
	Intsalela ye-E.Coli RNA	Q - I-PCR
	I-Avibiotics	Elisa
	Endotoxin	Indlela ye-1143 ye-CHP 2020
	Ukuncwina	Indlela i-1101 ye-chp 2020
Ukuziqhelanisa nokuzihlaziya	I-DNA uxinzelelo	Indlela 0401 ye-CHP 2020

* Qaphela: I-Hilgene eseka iindlela ze-QC ezihambelana namaqonga etekhnoloji eyahlukileyo, kunye neendlela ze-QC kubandakanya kodwa zingapheleli kwizinto zezinto ezingaphezulu.

Ixesha leprojekthi

Isicwangciso soLawulo lweProjekthi

Iqela leProjekthi yase-Rillgene, equka izazinzulu zeprojekthi, abaphathi beeprojekthi, iingcali zeProjekthi zePREP, ziya kwenza imizamo yokuqinisekisa ukusebenza kakuhle nokuvakalayo kweprojekthi nganye ye-GMP.

Khuphela

Izisombululo ze-CDM ezihlanganisiweyo zeMoto - Iiseli ze-T

Uphando lwakho alunakulinda - Kananjalo ayifanele isebenze!

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