Pichia pastoris DNA Residue Detection Kit(qPCR)

Pichia pastoris DNA Residue Detection Kit(qPCR)

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✓ ICH expert-developed, meets global regulatory standards (FDA/EMA/ChP)
✓ Ultra-high sensitivity: detects as low as 3 fg/μL DNA residue
✓ qPCR-based method (USP/Ph.Eur./ChP compliant)
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Overview
Protocols
Specifications
Shipping & Returns
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Overview

This qPCR-based kit delivers ultra-sensitive detection of Pichia pastoris host cell DNA residues (3 fg/μL LOQ), complying with FDA/EMA/ChP standards. Ideal for biopharma QC (vaccines, hormones, enzymes), it features a broad dynamic range (3–3×10⁵ fg/μL) and includes pre-treatment reagents. Validated for R&D and GMP production, it offers cost-effective performance versus imported alternatives. ISO 13485-certified

Usage Process



  1. Preparation                                           Sample Pre-treatment                  PCR Master Mix Preparation                            PCR Run                                            Data Analysis

    Q&A

    No. Issue Possible Causes Solutions
    1 No CT value Incorrect PCR program setup or fluorescence signal detection step Verify fluorescence channel selection and data collection settings in the program
    Degraded primers or probes Check primer/probe integrity via PAGE electrophoresis
    Degraded template or insufficient loading Avoid impurities during sample prep and minimize freeze-thaw cycles
    2 Poor standard curve Errors in DNA standard dilution, mixing, or pipetting (non-linear gradients) Double-check calculations during master mix preparation
    Degraded reference standards Ensure precise pipetting, avoid liquid retention on tips/walls, mix thoroughly (check ROX baseline), use appropriate dilution factors
    PCR inhibitors in template Limit freeze-thaw cycles of reference standards to manufacturer specifications

     





Cat.No. HG-HCP0010
Assay range 3-3E5 fg/μL
Precision R2≥0.990,Effective:85.0%-110.0%
Specification 100 reaction
LOD 3 fg/µL
LOQ 3 fg/µL
Bluekitbio is committed to providing the best products in the experimental research industry.
 

Shipping Information

We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.

 

 Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.

 

 Important Information

Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.

 

Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.

 

Shipping Issues: If you find that your package has not been delivered within the specified time; the tracking information shows that the package has been delivered but you have not received it; or your package includes missing or incorrect items or other logistics issues, please contact our customer service within 7 days of the payment date so that we can address these issues promptly.

 

Order Tracking

Once your order is shipped, you will receive an email with a tracking number and a link to track your shipment.

 
You can also track your order directly on our website by logging into your account and viewing your order history.

 

Shipping Restrictions

Please fill in the street address in detail, not PO BOX or military address (APO). Otherwise, we would have to use EMS for delivery (it's slower than others, taking around 1-2 months or even longer).

 

Customs Duties and Taxes Policy

Please note that any customs duties, taxes, or import fees incurred during shipping are the responsibility of the buyer. These charges vary depending on the destination country and are determined by local customs authorities.

By purchasing from our website, you agree to pay any applicable duties or taxes associated with your order. We are not responsible for delays caused by customs clearance.

 

Package Pickup Policy

Once your order has arrived at the designated pickup point or delivery location, please ensure prompt collection. If the package is not picked up within the designated time, we will send a reminder via email or SMS. However, if the package is not collected within the specified period, and any loss or damage occurs as a result, the buyer will be held responsible. We kindly remind you to collect your package promptly to avoid any potential issues.

Note:As our product falls under the special category, returns and refunds are not accepted.

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