Efficient Nuclease Detection with BlueKit's Benzonase ELISA Kit

Efficient Nuclease Detection with BlueKit's Benzonase ELISA Kit

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In the realm of molecular biology and diagnostics, the precision and efficiency of detecting nucleic acid-degrading enzymes – nucleases – are paramount. BlueKit is proud to introduce the Benzonase Nuclease ELISA Detection Kit, a state-of-the-art solution crafted for scientists who demand the utmost accuracy and reproducibility in their research. Our kit is designed to simplify the nuclease detection process, providing you with fast, reliable results that support the advancement of your scientific inquiries. The importance of nuclease detection in biomedical research cannot be overstated. Nucleases, which include both DNases and RNases, play crucial roles in various biological processes, including DNA replication, repair, and RNA processing. Their activity can be a double-edged sword; while essential for normal cellular functions, unregulated nuclease activity can lead to detrimental effects, including degradation of precious nucleic acid samples. This makes the detection and quantification of nuclease activity a critical step in many experimental protocols, particularly those involving sensitive techniques such as PCR, qPCR, and Next-Generation Sequencing. BlueKit's Benzonase Nuclease ELISA Detection Kit stands out due to its meticulously developed components and user-friendly protocol. The heart of our kit lies in its high-affinity antibodies, which have been rigorously tested to ensure unparalleled sensitivity and specificity for Benzonase nuclease. Unlike traditional nuclease detection methods that can be time-consuming and prone to variability, our ELISA-based approach streamlines the process, delivering quantifiable results with ease. The kit includes a pre-coated plate, standards, detection antibodies, and all necessary buffers and reagents, all optimized to work in unison to detect even the most minute levels of nuclease activity.

 

 

Standard curve

 

 

 

 

 

Datasheet

 



Furthermore, the kit boasts a robust standard curve, enabling precise quantification across a wide dynamic range. This feature is particularly beneficial for researchers looking to determine nuclease concentrations in various sample types, from cell lysates to purified enzyme preparations. Whether you're conducting basic research, developing therapeutic enzymes, or performing quality control assays, the Benzonase Nuclease ELISA Detection Kit from BlueKit provides the reliability and precision you need to succeed. In summary, BlueKit's offering is more than just a product; it's a catalyst for breakthroughs in the laboratory. It embodies our commitment to supporting the scientific community by providing tools that elevate the accuracy, efficiency, and reproducibility of their work. The Benzonase Nuclease ELISA Detection Kit is a testament to our dedication to quality, innovation, and the advancement of research. Choose BlueKit for your nuclease detection needs and experience the difference that precise, reliable results can make in your research endeavors.
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Cat.No. HG-BE001                                                         $1,508.00
 
This kit is designed for the quantitative detection of residual nuclease content in intermediates, semifinished products and finished products of various biological products by using a double-antibody sandwich method.


Performance

Assay range

  • 0.05-5ng/mL

 

Limit of quantitation

  • 0.05ng/mL

 

Limit of detection

  • 0.05ng/mL

 

Precision

  • CV%≤10%, RE%≤±15%

Instructions for Use of Nuclease ELISA Detection Kit Nuclease ELISA Detection Kit-Datasheet
FAQ
What is the optimal reaction temperature for this assay kit, and what happens if the temperature deviates from this range?

The optimal reaction temperature for this assay kit is 25℃. Deviating from this temperature range, either higher or lower, can lead to changes in detection absorbance and sensitivity. 

Can the components inside the assay kit be used directly, or are there any temperature-related requirements?

All components within the assay kit must be equilibrated to room temperature (20-25℃) before use. 

The kit is intended for scientific research only
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