E.coli HCP ELISA Detection Kit (2G)

E.coli HCP ELISA Detection Kit (2G)

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  1. Ultra-Sensitive E.coli HCP ELISA (0.3 ng/mL LOD)

✔Complete Antigen Coverage (100% E.coli HCPs Detected)
✔GMP-Ready Performance (CV<10%, 1-100 ng/mL Range)

 

 

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Overview
Protocols
Specifications
Shipping & Returns
Video Recording

Overview:

This kit is designed for the quantitative detection of HCP(host cell protein) content in biopharmaceuticals expressed on E.coli by using a double-antibody sandwich method. 

 

This kit can be used to detect all components of HCP(host cell protein) in E.coli.

Standard Curve


Datasheet


 

 


what is on the box?

Component Strength Preparation Storage temperature
Standards 1 mL × 8 tubes (ST1-ST7, NTC) Ready-to-use -20°C
100× Enzyme-labeled antibody 200 μL × 1 tube Dilute 1:99 with sample diluent -20°C
Sample Diluent 15 mL × 2 bottles Ready-to-use Unopened: -20°C; Opened: 2-8°C
20× Wash Buffer 25 mL × 2 bottles Dilute 1:19 with sterile water for injection Room temperature
Stop Solution 10 mL × 1 bottle Ready-to-use Room temperature
TMB Substrate A 6 mL × 1 bottle Ready-to-use Unopened: -20°C; Opened: 2-8°C
TMB Substrate B 6 mL × 1 bottle Ready-to-use Room temperature
Coated Plate 8 wells × 12 strips Ready-to-use Room temperature (with desiccant)
Sealer Film 5 pieces Ready-to-use Room temperature

Specification

Parameter Specification
Catalog No. IHG-HCP002-2G
Assay Range 1.5625-100 ng/mL
Sensitivity 0.3 ng/mL
Precision (Intra-assay) CV% < 10%
Accuracy RE% ≤ ±15%
Format 96-well plate
Storage Temperature -20°C
Bluekitbio is committed to providing the best products in the experimental research industry.
 

Shipping Information

We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.

 

 Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.

 

 Important Information

Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.

 

Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.

 

Shipping Issues: If you find that your package has not been delivered within the specified time; the tracking information shows that the package has been delivered but you have not received it; or your package includes missing or incorrect items or other logistics issues, please contact our customer service within 7 days of the payment date so that we can address these issues promptly.

 

Order Tracking

Once your order is shipped, you will receive an email with a tracking number and a link to track your shipment.

 
You can also track your order directly on our website by logging into your account and viewing your order history.

 

Shipping Restrictions

Please fill in the street address in detail, not PO BOX or military address (APO). Otherwise, we would have to use EMS for delivery (it's slower than others, taking around 1-2 months or even longer).

 

Customs Duties and Taxes Policy

Please note that any customs duties, taxes, or import fees incurred during shipping are the responsibility of the buyer. These charges vary depending on the destination country and are determined by local customs authorities.

By purchasing from our website, you agree to pay any applicable duties or taxes associated with your order. We are not responsible for delays caused by customs clearance.

 

Package Pickup Policy

Once your order has arrived at the designated pickup point or delivery location, please ensure prompt collection. If the package is not picked up within the designated time, we will send a reminder via email or SMS. However, if the package is not collected within the specified period, and any loss or damage occurs as a result, the buyer will be held responsible. We kindly remind you to collect your package promptly to avoid any potential issues.

Note:As our product falls under the special category, returns and refunds are not accepted.

Instructions for Use of E.coli HCP ELISA Detection Kit E.coli HCP ELISA Detection Kit-Datasheet
FAQ
What precautions should be taken during the addition of reagents to the microplate?

When adding reagents to the microplate, avoid touching the bottom of the wells to prevent damage to the coated layer. It is also important to change sample wells and tips between different samples and steps to prevent cross-contamination.

What should be kept in mind when washing the microplate strips, and can the sealing membrane be reused?

When tapping dry the strips after washing, care should be taken to prevent the strips from falling off. The sealing membrane should not be reused.

The kit is intended for scientific research only
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