Cell Cytotoxicity Assay Kit (Suspended Target Cells)
Cell Cytotoxicity Assay Kit (Suspended Target Cells)
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✔Dual-label cytotoxicity detection (CFSE+7-AAD)
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✔Optimized for suspension tumor cell lines
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✔Validated with CAR-T/NK cell therapies
Overview:
The Suspension Target Cell Cytotoxicity Assay Kit is designed to measure effector cell-mediated cytotoxicity against suspension-cultured target cells. The kit contains:
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CFSE (Carboxyfluorescein Succinimidyl Ester): A membrane-permeant green fluorescent dye for target cell identification in mixed populations
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7-AAD (7-Aminoactinomycin D): A DNA intercalator for labeling dead/damaged cells
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Culture Medium A & Assay Buffer
Applications
✔ Functional assessment of immune effector cells (NK cells, CAR-NK, CAR-T, TCR-T, Tm cells) against suspension tumor cell lines
✔ Process Development: Optimize cell therapy manufacturing workflows
✔ Quality Control: Release testing and stability studies for clinical-stage products
Key Features
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Dual-labeling system: CFSE (live targets) + 7-AAD (dead cells) for precise cytotoxicity quantification
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GMP-compatible: Validated for cell therapy development under FDA/EMA guidelines
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Workflow-ready: Pre-optimized protocols for seamless integration
Technical Highlights
▸ Target cell flexibility: Compatible with common suspension tumor lines (e.g., K562, Raji)
▸ Regulatory alignment: Supports IND-enabling studies with documentation packages
▸ Reproducibility: ≤10% CV in inter-assay precision testing
Datasheet:
Flow Cytometry Analysis of NK Cell-Mediated Cytotoxicity Against CFSE-Labeled K562 Target Cells
NK Cell Cytotoxicity Killing Curve Against CFSE-Labeled K562 Target Cells
| Catalog ID | Component | Quantity | Storage Conditions | Kit Location |
|---|---|---|---|---|
| HG-MD001 | Medium A | 100 mL | 2-8°C | Kit I |
| HG-FW001 | Assay Buffer | 100 mL | 2-8°C | - |
| HG-CFSE | CFSE Stain | 1 vial | -20°C | Kit II |
| HG-7-AAD | 7-AAD Stain | 1 vial | -20°C | - |
Specifition
| Performance Parameter | Acceptance Criterion |
| Accuracy | Recovery 70-130% |
| Robustness | Bias 70-130% |
| Repeatability | CV ≤ 10% |
| Intermediate Precision | CV ≤ 10% |
Shipping Information
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Important Information
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