Advanced E.coli Detection Kit for Accurate RT-PCR Analysis

Advanced E.coli Detection Kit for Accurate RT-PCR Analysis

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In the challenging landscape of molecular diagnostics, the detection of bacterial contaminants, particularly Escherichia coli (E. coli), is of paramount importance for ensuring the safety and quality of various biological samples. BlueKit's E.coli Residual Total RNA Detection Kit emerges as a pioneering solution, offering unparalleled precision and reliability in the field of RT-PCR based assays. Harnessing the power of real-time Polymerase Chain Reaction (RT-PCR) technology, this innovative kit is designed to provide rapid and sensitive detection of E. coli RNA, a crucial indicator of microbiological contamination. The meticulously developed standard curve, integral to the kit, guarantees accurate quantification, enabling researchers and quality control technicians to measure even minimal levels of E. coli with confidence.

 

 

Standard curve

 

 

 

 

Datasheet

 



At the core of BlueKit's E.coli Detection Kit is its user-friendly protocol, tailored to streamline the workflow in both research and industrial settings. From sample preparation to the final detection, each step is optimized for efficiency without sacrificing accuracy. This kit is not just an assay; it's a comprehensive solution for detecting E.coli, ensuring that your samples meet the highest standards of safety and quality. Ideal for a wide range of applications, including pharmaceutical manufacturing, food and beverage safety, and clinical diagnostics, the E.coli Residual Total RNA Detection Kit represents a critical tool in the modern laboratory's arsenal. By combining cutting-edge RT-PCR technology with unmatched ease of use, BlueKit provides a product that is not only a testament to innovation in molecular diagnostics but also a beacon of reliability for labs worldwide aiming to uphold the highest levels of integrity in their work.
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Overview
Protocols
Specifications
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Cat.No. HG-ER001                                                         $1,923.00

 

This kit is designed for the quantitative detection of residual E.coli total RNA in various biological products to improve control quality of nucleic acid.
 
This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR technology and fluorescent probe method, to realize one-step quantitative detection.


Performance

Assay range

  • 2.00 ~2.00×10fg/μL

 

Limit of quantitation

  • 2.00 fg/μL

 

Limit of detection

  • 0.50 fg/μL

 

Precision

  • CV%≤15%

Instructions for Use of E.coli Residual Total RNA Detection Kit (RT-PCR) E.coli Residual Total RNA Detection Kit (RT-PCR)-Datasheet
FAQ
What is the optimal reaction temperature for this assay kit, and what happens if the temperature deviates from this range?
  • The optimal reaction temperature for this assay kit is 25℃. Deviating from this temperature range, either higher or lower, can lead to changes in detection absorbance and sensitivity.
Can the components inside the assay kit be used directly, or are there any temperature-related requirements?
  • All components within the assay kit must be equilibrated to room temperature (20-25℃) before use.
The kit is intended for scientific research only
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