I-Viral Translations I / B / C (CRU / GMP)
I-Viral Translations I / B / C (CRU / GMP)
✔ I-zero cytotocity (Ukusebenza kwe-95% iposti - Unyango)
✔ Ibandakanya iseli - Iiprotokholi ezithile (T / i-NK / HSC ilindelwe)
Amagqabantshintshi:
I-BluekitI-Viral E - I-Hancer ™ Ngaba iklasi ye Ukuphakama - Ukusebenza kakuhle, Ayisiyo - Ukuphuculwa kwe-cytotooty kwi-lentivigraral kunye ne-verroviral veries. Ukuqesha a Indlela eyahlukileyo yesenzo, isebenza ngokubanzi kwi-chillral yogcino ngaphakathi Iiseli ze-T, iiseli ze-NK, kunye nee-HSCs.
I-Viral E - i-hancer ™ iphucula intsholongwane - I-cambrane fusion kunye nothutho lwe-intracellilla (ukusuka kwi-plasma membrane kwi-nucleus) ngo:
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Ukunciphisa i-vismrane vissity
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Ukukhuthaza ukutshintshiselana nge-lipid kunye nokurhweba ngokungekho mthethweni
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Ukubekwa kwengqondo yeProtiin
Izicelo
✔ Lunyhlise ukutshintshela ukusebenza kwe Iiseli ze-NK, iiseli ze-T, HSCs, kunye nezinye ezinzima - Ukususela - Iiseli ezidlulayo
✔ Ihambelana nayo IVSVG - kunye ne-baev - pseudoty I-lentivirus / retrovirus
Ukunciphisa iimfuno zomthwalo wentsholongwane (Ixabiso - Ukugcina)Ezona mpawu
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I-Boods yokutsalwa kwe-2-10X -
Iindleko zemveliso yabasezantsi Ngokunciphisa ukusetyenziswa kwe-lentivinicral
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Ukuqinisekisa ukhuseleko: Ilawula i-Viral Copy (VCN) yokuthobela Fda / i-EMA / NMPA Izikhokelo
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Iinketho eziguqukayo: Iyafumaneka kwi Ruo (uphando usebenzisa kuphela) kunye namabakala e-GMP Nge-R & D kwiRhafu - Ukuveliswa kwesikali
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Ukudibana okungagungqi: Iiprothokholi ezihle zodidi lweeseli ezahlukeneyo, ukuqinisekisa ukungqinelana okukhoyo
Ukuphuculwa kweLentivisral I-LetChoction kwiiseli ze-T nge-vill e - i-hancer a + b
I-TPREUR TUP - Utshintshiselwano lweseli: I-Viral E - I-hancer A + b ikhuphe ezinye iimakethi zentengiso (Khohlo) Imoto - Iiseli ze-T: Intsholongwane E - i-hancer A + i-B(Kunene) Imoto - Iiseli ze-T: Ulawulo olungachazwanga (Moi = 0.5)
Ukuphuculwa kotshintshiselwano lwe-lentiviral kwiiseli zeNK nge-vill e - i-hancer c
Uhlalutyo lokuthelekisa lwe-NK
: I-Viral E - I-hancer c vs. Abakhuphisana
Ukuphuculwa kotshintsho lweRetroviral kwiiseli ze-T nge-vill e - i-hancer c
Uhlalutyo lokuthelekisa le-T - Ukuhanjiswa kweselula (A) Imoto - Iiseli ze-T zidluliselwa Intsholongwane e - i-hancer c,(B) Imoto - Iiseli ze-T (ulawulo olungafundiswanga)
: I-Viral E - I-hancer c vs. Abakhuphisana (Moi = 5)
Ukuqulunqwa kwe-lentivivral ephuculweyo yee-HSCs kusetyenziswa intsholongwane e - i-hancer a + d indibaniselwano
Uhlalutyo oluthelekisayo lwe-HSC
| Inombolo | Igama lezinto ezinokuthengiswa ngokutsha | Isixa (umz. Zintandathu - ipleyiti kakuhle) |
| 1 | Intsholongwane | Njengoko kufuneka |
| 2 | Inkcubeko | Njengoko kufuneka |
| 3 | Isithandathu - ipleyiti kakuhle | 1 |
| 4 | I-TOSK | 1 |
| 5 | I-T75 FLAASK | 2 |
| 6 | I-T175 flask | 2 |
| 7 | I-15 ml centrifuge tube | Iqela |
| 8 | I-50 Ml Centrifuge Tube | Iqela |
| 9 | I-10 ml pipette | Iqela |
| 10 | I-25 ml pipette | Iqela |
| 11 | I-1 ML Pipette Tips | Iqela |
| 12 | Iingcebiso ezingama-200 μl pipette | Iqela |
Ulwazi lokuhambisa
Sinikezela ngothutho oluhlaziyiweyo kuyo yonke imiyalelo. Ngokwesiqhelo, iodolo yakho iya kufika ngaphakathi kwe-5 - Iintsuku ezisi-7 zeshishini eUnited States kwaye kwiintsuku ezili-10 zomsebenzi wamazinye amazwe. Nangona kunjalo, nceda uqaphele ukuba ukuhanjiswa kweendawo ezisemaphandleni kunokuthatha ixesha elide.
Ixesha lokuhambisa: Ii-odolo zihlala zisetyenzwa ngaphakathi 1 - iintsuku ezintathu zomsebenzi. Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile yokuqinisekisa ngolwazi lokulandela umkhondo.
Ulwazi olubalulekileyo
Ukuqhubekeka kocwangco: Emva kokuba i-odolo ihlawulwe, i-Warehouse yethu idinga ixesha lokuqhuba iodolo yakho. Uya kufumana isaziso emva kokuba i-odolo yakho ithunyelwe.
Amaxesha okuhambisa: Kwiimeko ezininzi, iphakheji iya kusiwa ngexesha eliqikelelweyo lokufika. Nangona kunjalo, umhla wokwenyani unokuchaphazeleka ngamalungiselelo eenqwelomoya, iimeko zemozulu, kunye nezinye izinto zangaphandle. Ixesha lokuhanjiswa kwexesha liya kuba ngaphezulu kwesiqhelo semiyalelo ebandakanya ukhuseleko okanye izinto ezenziwe ngokwezifiso. Nceda ujonge ulwazi lokulandelela ngowona mhla uchanekileyo wokuhanjiswa.
Imicimbi yokuhambisa: Ukuba ufumanisa ukuba iphakheji yakho ayihanjiswanga ngexesha elimiselweyo; Ulwazi lokulandela umkhondo lubonisa ukuba iphakheji ihanjisiwe kodwa awukayifumani; Okanye iphakheji yakho ibandakanya izinto ezilahlekileyo okanye ezingachanekanga okanye eminye imiba yempahla, nceda uqhagamshelane nenkonzo yethu yabathengi kwiintsuku ezisi-7 zomhla wokuhlawula ukuze sikwazi ukujongana nale micimbi ngokukhawuleza.
Ukulandelela umkhondo
Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile ngenombolo yokulandela umkhondo kunye nekhonkco lokulandelela ukuthunyelwa kwakho.
Unokulandelela i-odolo yakho ngqo kwiwebhusayithi yethu ngokungena kwiakhawunti yakho kwaye ujonge imbali yakho ye-oda.
Izithintelo zokuhambisa
Nceda ugcwalise idilesi yesitrato ngokweenkcukacha, hayi ibhokisi ye-PO okanye idilesi yomkhosi (AP). Ngaphandle koko, kuya kufuneka sisebenzise i-EMS yokuhanjiswa (ihamba kancinci kunabanye, ukuthatha malunga 1 - iiyure ezi-2 okanye ixesha elide).
Imisebenzi yesiko kunye nomgaqo-nkqubo werhafu
Nceda uqaphele ukuba nayiphi na imisebenzi yesiko, irhafu, okanye intlawulo yokungenisa okwenziweyo ngexesha lokuhambisa luxanduva lomthengi. Ezi ntlawulo ziyahluka ngokuxhomekeke kwilizwe eliya kulo kwaye zimiselwa ngabasemagunyeni basekhaya.
Ngokuthenga kwiwebhusayithi yethu, uyavuma ukuhlawula nayiphi na imisebenzi esebenzayo okanye irhafu enxulumene ne-oda yakho. Asinaxanduva lokulibaziseka okubangelwa kukucocwa kwesiko.
Umgaqo-nkqubo wePakeji yephakheji
Nje ukuba i-odolo yakho ifikile kwindawo echongiweyo yePickup okanye indawo yokuHanjiswa, nceda uqinisekise ukuqokelelwa ngokukhawuleza. Ukuba iphakheji ayithanga ngexesha elichongiweyo, siya kuthumela isikhumbuzo nge-imeyile okanye nge-SMS. Nangona kunjalo, ukuba iphakheji ayiqokelelwa ngaphakathi kwexesha elichaziweyo, kwaye nayiphi na ilahleko okanye umonakalo ovela ngenxa yoko, umthengi uya kuba noxanduva. Ngobubele kukhumbuza ukuba uqokelele iphakheji yakho ngokukhawuleza ukuphepha nayiphi na imiba enokubakho.
Qaphela: Njengoko imveliso yethu iwela phantsi kodidi olukhethekileyo, ibuyise kunye nokubuyiselwa kwemali ayamkelwa.
