Trancration Tranction e ntlafatsa a / b / c (rou / gmp)
Trancration Tranction e ntlafatsa a / b / c (rou / gmp)
✔ Zero STOToxity (Ho hlokahala> PUSELETSO EA 95% - Phekolo)
✔ E kenyelletsa sele - Protocol tse tobileng (T / NKT / HSC e hlophisitsoe)
Kakaretso:
BluekitViral E - Seriti sa Hacer ™ ke sehlopha sa phahameng - katleho, e seng - ntlafatso ea "cytotox" bakeng sa lijana tsa lepono tsa sesole le tsa khutla. Ho soma a Mechine e Ikhethileng ea Ketso, e sebetsa ka bongata bakeng sa phetoho ea vaerase ho Lisele tsa Tk, lisele tsa NK le HSC.
Viral E - Hacer ™ e ntlafatsa Vaerus - selefounu ea selefounule lipalangoang tsa intracwar (ho tloha plasma membrane ho Nucleus) ka:
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Ho fokotsa pono ea membrane
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Ho khothaletsa Lipid Lipid & Transmembrane Transkiking
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Polelo ea protheine e fetolang ka botlalo
Likopo
✔ Fihlisa katleho ea Transation bakeng sa Lisele tsa NK, lisele tsa TS, HSCS, le tse ling tse thata - ho sebelisa lisele
✔ tšoanellehang le Vsvg - le baeva - pseudottityped Lenntivuruse / Retrovirussus
✔ Fokotsa litlhoko tsa vaerase tse boima (Liaparo - Boloka)LINTHO TSE HLAHANG
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E etsa hore ts'ebetso ea ho tlisoa ke 2-10x -
Litsenyehelo tsa tlhahiso ea Liporete Ka ho fokotsa ts'ebeliso ea letintšana
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Netefatsa polokeho: Taolo ea Giral Copy Copy (VCN) ho latela FDDA / EMA / NMA Tataiso
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Likhetho tse feto-fetohang: E fumaneha ho Ruo (ho sebelisa lipatlisiso feela) le gmp limaraka Bakeng sa R & D ho Commercial - tlhahiso ea tlhahiso
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Ho hokahana ha leoatle: Li-protocol tse ntlafalitsoeng bakeng sa mefuta ea selefouno, ho netefatsa ka mokhoa o hlakileng le lihlahla tse teng
Matlafatso ea ho Tlisoa ho lisele tsa T lisele tsa T li-viral e - Hancer a + b
Pleior T - Transduction Transduction: Viral E - Hancer A + B andayrmals Maraka (Ka ho le letšehali) Car - Lisele tsa T lisele: Viraral ee - Hancer a + B kalafo,(Ka ho le letona) Koloi - Lisele tsa T: Taolo e sa rutoang (Moi = 0.5)
Ntlafatso ea ho tshwantsha ho lisele tsa NK ka viral e - Hacer C
Tlhahlobo e bapisang ea ts'ebetso ea Trancon ea NK Celder ea Trancence e setseng: Ho phalla Cytometry ea I15 NK ByTometry ea I15 NK e phekoloa ka Viraral E - Hancer C
: Viral E - Hancer C Alcer C
Ntlafatso ea Trancolotion ea ho emisa ho lisele tsa T lisele tsa TRI ka viral e - Hacer C
Tlhahlobo e patehileng ea T - Ho qala ka Cef Transduducticiniency (A) Koloi - lisele tse fetotsoeng Viral E - Hacer C,(B) Koloi - Lisele tsa T-T (taolo e se nang thuso)
: Viraral E - Hancer C Vs. Bahlolisisi (Moi = 5)
Trancent e ntlafalitsoeng e matlafatsang ea HSCS e sebelisang viral e - hancer a + d
Tlhahlobo e bapisang ea katleho ea HSC e tšoantšetso: Viral e - hancer a + ds "ea mmaraka (moi = 5)
| Palo | Lebitso la likhohlano tsa khale | Palo (mohlala. Thoteng ea rona) |
| 1 | Vaerase | Joalokaha ho Hlokahala |
| 2 | Setso sa Sebetsa | Joalokaha ho Hlokahala |
| 3 | Tse tšeletseng - poleiti e hantle | 1 |
| 4 | T25 Folask | 1 |
| 5 | T75 Folask | 2 |
| 6 | T175 Flask | 2 |
| 7 | 15 ml centrisuge tu tube | Tse 'maloa |
| 8 | 50 ml centrisuge tu tube | Tse 'maloa |
| 9 | 10 ml Pipette | Tse 'maloa |
| 10 | 25 ml Pipette | Tse 'maloa |
| 11 | 1 ml Pipette Malebela | Tse 'maloa |
| 12 | Malebela a 200 μl Pipette | Tse 'maloa |
Tlhahisoleseling ea ho tsamaisa
Re fana ka lipalangoang tse sephara ho litaelo tsohle. Ka tloaelo, odara ea hau e tla fihla leqepheng la 5 - matsatsi a khoebo a 7 United States le matsatsing a 10 a khoebo bakeng sa linaha tse ling. Leha ho le joalo, ka kopo hlokomela hore ho isoa ho libakeng tsa mahaeng ho ka nka nako e telele.
Nako ea ho tsamaisa: Litaelo li sebetsoa nakong ea 1 - matsatsi a 3 a khoebo. Hang ha odara ea hau e se e rometsoe, u tla fumana lengolo-tsoibila le netefatsang ka tlhaiso-leseling.
Tlhahisoleseling ea Bohlokoa
Ho sebetsa ha taolo: Kamora hore taelo e se e lefiloe, ntlo ea rona ea bophelo e hloka nako ea ho sebetsa odara ea hau. U tla fumana tsebiso hang ha odara ea hau e rometsoe.
Mefuta ea ho tsamaisa: Maemong a mangata, sephutheloana se tla tsamaisoa ka nako e lekantsoeng ea ho fihla. Leha ho le joalo, letsatsi la pele la tlhahiso le ka angoa ke litokisetso tsa lifofane, maemo a leholimo le lintho tse ling tsa kantle. Nako ea ho tsamaisa thepa e tla be e le telele ho feta e tloaelehileng bakeng sa litaelo tse kenyelletsang mokhoa oa pele kapa lintho tse hlophisitsoeng. Ka kopo sheba tlhaiso-leseling ea ho batla bakeng sa letsatsi le nepahetseng ka ho fetisisa.
Litaba tsa thomello: Haeba u fumana hore sephutheloana sa hau ha se so lopolloe ka nako e boletsoeng; Tlhahisoleseling ea ho lelekisa e bontša hore sephutheloana se pholositsoe empa ha u e amohetse; Kapa sephutheloana sa hau se kenyelletsa lintho tse sieo kapa tse fosahetseng kapa mathata a mangata, ka kopo ikopanye le ts'ebeletso ea bareki nakong ea matsatsi a 7 e le hore re ka sebetsana le litaba tsena hang-hang.
Ho latela tumello
Hang ha odara ea hau e romelloa, u tla fumana lengolo-tsoibila le nang le nomoro ea ho batla le sehokelo sa ho latela thomello ea hau.
U ka latela odara ea hau ka kotloloho webosaeteng ea rona ka ho kena akhaonteng ea hau mme u shebe nalane ea hau ea odara.
Lithibelo tsa ho tsamaisa
Ka kopo tlatsa aterese ea seterateng ka botlalo, eseng lebokose la Pox kapa aterese ea sesole (APP). Ho seng joalo, re tla tlameha ho sebelisa ems bakeng sa ho tsamaisa (e ea lieha ho feta tse ling, ho nka 1 - ho nka likhoeli tse peli kapa nako e telele.
Mesebetsi ea Litloaelo le Tafole ea Lekhetho
Ka kopo elelloa hore mesebetsi efe kapa efe ea lekhetho, lekhetho kapa litefo tsa ho tlisa thepa tse hlahisitsoeng nakong ea thomello ke boikarabello ba moreki. Litefiso tsena li fapana ho latela naha e eang 'me li khethoa ke ba boholong sebakeng sa lehae.
Ka ho re reka webosaeteng ea rona, o lumela ho lefa mesebetsi efe kapa efe e sebetsang kapa lekhetho le amanang le odara ea hau. Ha re ikarabelle bakeng sa ho lieha ho bakoang ke tumello ea litloaelo.
Phokotso ea "PUSELETSO EA PUSO
Hang ha odara ea hau e fihlile sebakeng se khethiloeng sa Pionep Point kapa sebaka sa ho tsamaisa, ka kopo netefatsa pokello ea potlako. Haeba sephutheloana se sa nkuoe ka nako e behiloeng, re tla romella khopotso ka lengolo-tsoibila kapa sms. Leha ho le joalo, haeba sephutheloana se sa bokelloa nakong e boletsoeng, 'me tahlehelo e teng kapa tšenyo e etsahala ka lebaka leo, moreki o tla jara boikarabelo. Re u hopotse ka mosa hore u bokelle pakete ea hau hang-hang ho qoba litaba life tse ka bang teng.
Tlhokomeliso: Ha sehlahisoa sa rona se oela tlasa sehlopha se khethehileng, ho khutla le theko ha se amoheloe.
