Viral transduction enhancer a / b / c (rou / gmp)
Viral transduction enhancer a / b / c (rou / gmp)
✔ Zero cytototoxity (ViaBility> 95% Post - Kurapa)
✔ Inosanganisira cell - chaiyo protocol (T / NK / HSC yakagadziriswa)
Overview:
BluekitViral E - Hancer ™ Series kirasi ye Yakakwira - Kubudirira, Kwete - Cytotoxic Transduction Inhaucers for lentivaram uye kudzoreredza vhezheni. Kusunungura a Mhando yakasarudzika yekuita, zvinowanzoshanda zvakanyanya kune viratiction vhezheni mukati T masero, nk masero, uye hscs.
Vira e - Hancer ™ inosimbisa hutachiona - Cell MeMbrane Fusion uye intracellular kutakura (kubva plasma membrane kune nucleus) na:
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Kuderedza membrane viscosity
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Kusimudzira LIPID Exchange & Transmemrane Kutengesa
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Chinyararwi chinosimbisa nzvimbo protein kutaura
Zvishandiso
✔ Inosimudzira Shanduko Kubudirira kwe Nk masero, t masero, hscs, uye zvimwe zvakaoma - kusvika - transduce masero
✔ kuenderana VSVG - and baev - pseudotyped Lentivirus / Retroviruses
✔ inoderedza zvivakwa zvekutakura zvinodiwa (Mutengo - Kuchengetedza)ZVINOKOSHA ZVINOKOSHA
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Inosimudzira kushandurwa kwekubudirira nekubudirira na 2-10x -
Inobvisa mari yekugadzira Nekuderedza kushandisa lentivaram
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Inosimbisa kuchengetedzwa: Kudzora Viral Copy nhamba (VCN) kutevedzera FDA / EMA / Nmpa Nhungamiro
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Sarudzo dzinochinja: Inowanikwa mukati Ruo (Kutsvaga Shandisa chete) uye GMP Giredhi for r & d kune yekutengesa - kujaira kugadzirwa
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Kusanzwisisika kusanzwisisika.
Lentivaram Transduction kuvandudza mu t masero naVaira e - Hancer A + B
Superor T - Cell Transduction: VIRIRA E - HANCER A + B Outperforms Market Alternatives (Ruboshwe) CAR - T Masero: Viral E - Hancer A + B Kurapwa,(Rudyi) Car - T Masero: Asina kutapwa Kudzora (Moi = 0.5)
Kusimudzira kwekushandurwa kweLentivirish muNK masero naVIRIRA E - Hancer C
Kuongorora kweNK Shandura Kubudirira Kubudirira Kureba: Kuyerera Cytometry yeI15 NK Masero Akabatwa neVIRIRA E
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Kuwedzera kwekudzoreredza kushandurwa mu t masero naVIRA E - Hancer C
Kufananidza kuongorora kweT - cell transduction inoshanda (A) Car - T Masero anoshandurwa na Viral e - Hancer C,(B) CAR - T masero (zvisingaitwi kutonga)
: Vira e - Hancer C vs. kukwikwidza (moi = 5)
Yakavandudzwa LEntivirish Shanduro yeHSCs uchishandisa virral e - Hancer A + D kusanganiswa
Kufananidza kuongororwa kweHSC transduction Kubudirira: Viral e - Hancer A + D VS VS MALECATIVES (MoI = 5)
| Nhamba | Zita reReget Conslealts | Huwandu (semuenzaniso. Natanhatu - Zvakanaka Plate) |
| 1 | Utachiona | Sezvazvinodiwa |
| 2 | Tsika Pakati | Sezvazvinodiwa |
| 3 | Matanhatu - PEDYO NEMAHARA | 1 |
| 4 | T25 FLASK | 1 |
| 5 | T75 FLASK | 2 |
| 6 | T75 FLASK | 2 |
| 7 | 15 ml Centrifigage Tube | Dzinoverengeka |
| 8 | 50 ml centrifuge tube | Dzinoverengeka |
| 9 | 10 ml pipiette | Dzinoverengeka |
| 10 | 25 ml pipiette | Dzinoverengeka |
| 11 | 1 ml pipiette matipi | Dzinoverengeka |
| 12 | 200 μl Pipiet Matipi | Dzinoverengeka |
Kuendesa ruzivo
Isu tinopa firiji kutakura pane ese mairairo. Kazhinji, yako oda ichasvika mukati me5 - 7 mazuva ebhizinesi muUnited States uye mukati memazuva gumi ebhizinesi kune dzimwe nyika. Nekudaro, ndapota ona kuti kuendesa kunzvimbo dzekumaruwa kunogona kutora nguva yakareba.
Nguva yekutumira: Mahoridhe anowanzoitwa mukati me1 - 3 mazuva ebhizinesi. Kamwe kuraira kwako kwatumirwa, iwe uchagamuchira email yekusimbisa neruzivo rwekutsvaga.
Ruzivo rwakakosha
Kurongeka Kugadzirisa: Mushure mekunge kurongeka kwakabhadharwa, yedu yekuchengetedza inoda imwe nguva yekugadzirisa yako oda. Iwe uchagamuchira chiziviso kana yako odha yatumirwa.
Nguva dzekutumira: Muzhinji, iyo package ichaunzwa mukati menguva inofungidzirwa kusvika. Nekudaro, zuva chairo rekutumira rinogona kukanganiswa nehurongwa hwekubhururuka, mamiriro ekunze, uye zvimwe zvinhu zvekunze. Iyo nguva yekutumira yenguva ichave yakareba kupfuura yenguva dzose yemirairo inosanganisira preorder kana yakagadzirirwa zvinhu. Ndokumbira utarise kune iyo yekutsvaga ruzivo rweiyo chaiyo yekuzvitakura zuva.
Nyaya dzekutumira: Kana iwe ukaona kuti yako package haina kuendeswa mukati menguva yakatarwa; Ruzivo rwekutsvaga runoratidza kuti pasuru yakanunurwa asi hauna kuigamuchira; Kana kuti package yako inosanganisira yakarasika kana isiriyo zvinhu kana zvimwe zvinhu zveongororo, ndapota bvunza yedu yevatengi basa mukati memazuva manomwe ezuva rekubhadhara kuitira kuti titaure nyaya idzi nekukurumidza.
Kurongeka kwekutevera
Kamwe kuraira kwako kwatumirwa, iwe uchagamuchira email ine nhamba yekutevera uye chinongedzo kuti utarise kutumira kwako.
Iwe unogona zvakare kutarisa yako odha zvakananga pane yedu webhusaiti nekupinda muakaunti yako uye kutarisa yako yekuraira nhoroondo.
Kutumira Zvirambidzo
Ndokumbirawo zadza kero yemugwagwa mune ruzivo, kwete po bhokisi kana kero yemauto (apo). Zvikasadaro, isu taizofanira kushandisa EMS yekuzvitakura (inononoka kupfuura vamwe, kutora nguva 1 - 2 mwedzi kana zvakare kureba).
Mabasa ezvekutengesa uye mutero mutemo
Ndokumbira utarise kuti chero mabasa ezvetsika, mutero, kana mari yekunongedza yakavezwa panguva yekutumira ibasa remutengi. Aya anopomerwa zvinosiyana zvichienderana nenyika yekuenda uye inotemerwa nenzvimbo dzemunharaunda yemunharaunda.
Nekutenga kubva pawebhusaiti yedu, unobvuma kubhadhara chero mabasa anoshanda kana mutero unobatanidzwa neyako oda. Isu hatisi mhaka yekunonoka kunokonzerwa netsika clearance.
Package pickup mutemo
Kamwe kuraira kwako kwasvika pane yakasarudzwa pickup point kana nzvimbo yekuzvitakura, ndapota ita chokwadi chekureva kuunganidzwa. Kana iyo package ikasatorwa mukati menguva yakatarwa, tichazotumira chiyeuchidzo kuburikidza neemail kana SMS. Nekudaro, kana iyo package ikasaunganidzwa mukati menguva yakatarwa, uye kurasikirwa kana kukuvara kunoitika semhedzisiro, mutengi achazotarisirwa. Isu tinokuyeuchidza nemufaro kuti utore yako package nekukurumidza kudzivirira chero nyaya dzinogona kuitika.
Ongorora: Sezvo chigadzirwa chedu chinowira pasi pechikamu chakakosha, kudzoka uye kudzoserwa hazvigamuchirwe.
