Virral Conveduction Faʻaleleia A / B / C (RU / GMP)
Virral Conveduction Faʻaleleia A / B / C (RU / GMP)
✔ Zero cytotoxictity (lautele> 95% pou - Togafitiga)
✔ Aofia ai telefoni - faʻapitoa faʻapitoa (T / NK / HSC Exptimized)
Vaaiga lautele:
BluekitViral u - hancer ™ faasologa o se vasega o Maualuga - Lelei le lelei, Nono - Cytotoxic Transcurity Emplocers mo le lentiviral ma retroviral vectors. O le tulaga ese faiga o gaioiga, e sili atu ona aoga mo le ogatotonu T sela, NK sela, ma HSCS.
Viral u - hancer ™ faʻaleleia atili Viraus - Cell Mixrane Fusion ma felauaiga intracerucular (mai le plasma membrane i nucleus) e:
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Faaitiitia Mimbrane Viscosity
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Faʻalauiloa LIPID Fesuiaiga & TransmemmBrane fefaʻatauaʻi
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Faʻaopopoina modulating luga poʻo le porotini faʻaaliga
Faatatauga
✔ Faʻaleleia atili le faʻatulagaina o le lelei NK sela, t sela, hcs, ma isi mea faigata - i - transduce sela
✔ fetaui ma VSVG - ma baev - pseudotyped Lentivirusses / Retrovirses
✔ Faʻaititia Vral Age Manaʻoga (Tau - Faasaoina)Manatu Autu
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Faʻateleina le faʻaliliu e fetaui lelei ma le 2-10x -
Lowers gaosiga tau I le faʻaititia o le le aʻoga
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Mautinoa le saogalemu: Puleaina VRAL COMPAD numera (vcn) e tausisi ai FDA / EMA / NMPA taiala
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Fepleble filifiliga: Avanoa i Rudo (suʻesuʻega faʻaaoga na o le) ma gmp togi Mo R & D i le pisinisi - fua gaosiga
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Le aoga le tuʻufaʻatasia: Faʻalelei Prtocols mo eseese ituaiga sela, mautinoa le talafeagai ma galuega o loʻo iai nei
Lentivicial Transduction Faʻaleleia atili i le LLells i Viral E - HAYR A + B
Sili atu t - Cell Conveduction: Viral u - Hancer A + B Outperform Mart Sides (Taumatau) Car - T sela: Viral u - hancer A + B togafitiga,(Tauagavale) Car - T sela: le le fiafia o le pule (Moi = 0.5)
Faʻaleleia o le lentiviral Transduction i NK sela e le viral u - hancer c
Faʻatusatusaga auiliiliga o le NK Cell Conveduction Sporcation Steectiction Shie: O le RID CYTOMETHER O I15 NK sela e togafitia ai le - HOYR C
: Vinals u - hancer c vs. Tauvaga (moi = 1) taumatau: tafe cytometry o I15 nk sela (le togafitia chums (le fiafia o sela)
Faʻaleleia o le toe faʻaleleia o le toe faʻafoʻi i totonu o sela e viral e - hancer c
Faatusatusaga auiliiliga o le t - Cell Conveduction Lelei (A) Taavale - t sela faaliliu ma Viral u - hancer c,(B) Taavale - T sela (le togafitia)
: Vinals u - hancer c vs. Tauvaga (moi = 5)
Faʻalauteleina le faʻauiga o le le mautonu o le HSCS e faʻaaoga ai viral u - hancer a + o le tuʻufaʻatasiga
Faatusatusaga auiliiliga o le HSC Transduction Emplocation: Vierals E - HOYER A + D OR. Active Swevets (Moi = 5)
| Numera | Igoa o le Reagnt | Aofaiga (eg. Ono - lelei ipu) |
| 1 | Vairusi | A manaomia |
| 2 | Aganuu auala | A manaomia |
| 3 | Ono - lelei le ipu | 1 |
| 4 | T25 flask | 1 |
| 5 | T75 flask | 2 |
| 6 | T175 flask | 2 |
| 7 | 15 ml centrifuge tube | Tele |
| 8 | 50 ml centrifuge tube | Tele |
| 9 | 10 ml paipa | Tele |
| 10 | 25 ml paipa | Tele |
| 11 | 1 ml pipette kips | Tele |
| 12 | 200 μl Paipette Fesoasoani | Tele |
Vaa Faamatalaga
Matou te ofoina atu le tausiaina o felauaiga i luga o poloaiga uma. E masani lava, o lau faʻatonuga o le a taunuʻu i totonu o le 5 - 7 aso pisinisi i le Iunaite Setete ma i totonu o le 10 aso pisinisi mo isi atunuʻu. Peitaʻi, faʻamolemole matau o loʻo lafoina i nuu i tua e ono umi se umi.
Vaaf Time: Poloaiga e masani ona gaosia i totonu 1 - 3 aso pisinisi. O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se faʻamaoniga imeli ma le sailia o faʻamatalaga.
Faamatalaga Taua
Poloaiga Faʻatau: A maeʻa le faʻatonuga, o loʻo manaʻomia e la matou fale teu oloa i se taimi e faʻatautaia ai lau faʻatonuga. O le a e mauaina se faasilasilaga pe a uma ona lafo lau poloaiga.
Faʻalauiloaina taimi: O le tele o taimi, o le afifi e tuʻuina atu i totonu o le Tala Faatatau o Taunuu. Peitaʻi, o le aso faʻaolaina moni e mafai ona aʻafia i le faʻatulagaina o le faigamalaga, o tulaga o le tau, ma isi mea i fafo. O le faʻatulagaina o taimi faʻatulagaina o le a umi atu nai lo le masani ai mo poloaiga e aofia ai le faʻatonuga poʻo mea faʻapitoa. Faʻamolemole tagaʻi i le sailia o faʻamatalaga mo le sili ona saʻo le tiliva.
Lafo mataupu: Afai e te maua o lau afifi e le i tuʻuina atu i totonu o le taimi faʻapitoa; O le sailia o faʻamatalaga e faʻaalia ai na tuʻuina atu le afifi ae e te leʻi mauaina; poʻo lau afifi e aofia ai le leiloa poʻo le saʻo aitema poʻo isi mataupu faʻapitoa, faʻamolemole faʻafesoʻotaʻi a matou tagata faʻatau auaunaga i totonu o le 7 aso o le Aso e tatau ai.
Poloaiga Ova
O le taimi lava e lafoina lau faʻatonuga, o le ae mauaina se imeli ma se numera numera ma se fesoʻotaʻiga e siaki lau uta.
E mafai foi ona e siakiina lau poloaiga i luga o la matou 'upega tafaʻilagi e ala i le ulufale i totonu o lau teugatupe ma vaʻai i lau faʻatonuga talaʻaga.
Tape tapulaa
Faʻamolemole faʻatumu i le auala auala auiliili, ae le o le PO Box poʻo le tuatusi militeli (APA). A leai, o le a tatau ona matou faʻaaogaina EMS mo le momoliina atu (e telegese nai lo isi, e fealuaʻi i le 1 - 2 masina pe sili atu).
Tiute Tiute ma Lafoga mo lafoga
Faamolemole matau o tiute o agaifanua, lafoga, po o totogi faaulufale mai, po o totogi faaulufale i le taimi o le lafoina o le matafaioi a le tagata faatau. O nei tuʻutuʻuga nei e eseese faʻalagolago i le atunuʻu nofoaga ma ua fuafuaina e le au faʻaleaganuʻu.
I le faʻatauina mai o la matou 'upega tafaʻilagi, ua e malie e totogi ni tiute talafeagai poʻo lafoga e fesoʻotaʻi ma lau poloaiga. Matou te le nafa ma le tuai ona mafua mai i le kiliaina o le Tiute.
Afifi faiga
O le taimi lava e taunuʻu ai lau faʻatonuga i le filifilia o le piki poʻo le mea e momoli ai, faʻamolemole ia mautinoa le pasiaina o le aoina. Afai o le afifi e le pikiina i totonu o le taimi atofaina, o le a matou lafoina se faʻamanatu e ala i imeli poʻo SMS. Peitaʻi, pe a le aoina le afifi i totonu o le vaitaimi faʻapitoa, ma soʻo se leiloa poʻo se faʻaletonu e tupu mai i le mea e tupu mai ai, o le tagata faʻatau o le a nafa ma le tagata faʻatau. Matou te faʻamanatu lelei atu ia te oe e aoina vave lau afifi e 'alofia ai ni faʻafitauli o ni mataupu.
Faʻamatalaga: A o le a matou oloa e paʻu i lalo o le faʻapitoa vaega, toe faʻafoʻi ma toe faʻafoʻi e le taliaina.
