Ntchito za CDMO kwa Plasmids - IIT Gawo / No - Gawo Lachipatala

Kupanga ma plasmids, gawo lofunikira kwambiri pakupanga galimoto - Tsekani mankhwala othandizira, zimaphatikizapo njira zingapo zovuta zopanga, kuyeretsedwa, ndi kusanthula. Monga zida zofunikira za uneneti urnetic, ma gssteria ma plasmuds sangathe kugwiritsidwa ntchito ngati zinthu zomaliza za gene ndi ma cell othandizira, komanso zopangidwa ndi ma cell. Ndi kutuluka kwa mapangidwe a cellular, misika yamsika ikuwonjezeranso chaka chilichonse. Mapiri ali ndi mwayi pakukonza njira yophatikizira ya CDMO ya ma cell othandizira ma cell, akhazikitsa nsanja ya acid a acid zopangidwa ndi zigawo za zigawo za ma plasmids kwa makasitomala omwe amafunidwa.

Ntchito

Ntchito za CDMO kwa ma plasmids
Mitundu			Ntchito
Iit kalasi	1	Palokha adapanga anayi - Pulogalamu ya Plasmid	● Mbadwo wachitatu 0 - Plasmid Dongosolo ● Kanamycin - kukana Gene ● Palibe chiphaso cha patent chofunikira	● Kulumikizana mwachisawawa kupita ku kugonjera ● GMP - monga zokambirana ● GMP - Monga dongosolo laudindo labwino ● Zolemba zowona ndi zowoneka bwino
	2	Kulengedwa kwa Bacteria Bank (GMP - monga)	● Nambala yokhazikika ndi kutanthauzira kwa mabakiteriya kuti apangidwe
	3	Kupanga Plasmid ndikuyesa (gmp - monga)	● Zotsatira zopanga ndi zonena

* Dziwani: Timapereka kusintha kosinthika ndi kusinthasintha kwa ntchito, kuphatikiza koma osangokhala pa ntchito.

Ubwino

Ubwino Wathu Wa Plasmid:

• Makina anayi odziyimira pawokha - Plasmid dongosolo ndi Kanamycin - kukana Mphete

• Dongosolo lokhala ndi kuthekera kwa kukhathamiritsa

• Makina ogwirira ntchito achita bwino, amagwirizana ndi zofunikira, komanso zoyenera

• Zokumana nazo zowonjezera zomwe zikuyenda bwino

• Galimoto - TVE ZOPHUNZITSIRA KUGWIRA NTCHITO PAMENE PANO NDIPONSO KUGWIRA NTCHITO

• 2 - 5 amalumikiza okwera okwera atatha kugwiritsa ntchito makina athu a plasmid kuchokera pakuyerekeza ma projekiti angapo

Ubwino wa Kupanga Plasmid yathu:

• Opanda maantibayotiki pamakonzedwe onse opanga

• Kupanga ma presmid ndi banki yopanga zokambirana zingapo

• Kudzipatula kwathunthu pakati pa osabala komanso osabala

• Kuyika zinthu zomaliza pogwiritsa ntchito iyolator

• Malizitsani ma CTD Dossiers kuti anyamule plasmid (ya lentivil verctor), kuchepetsa nthawi yobwereza 3 - Miyezi 4, yokhala ndi zinthu zochepa zomwe zimaperekedwa kale

Kupanga

Kuwongolera kwapadera

Chiyeso		Njira Yoyesera
Kaonekedwe		Kuyang'ana Zowoneka
Kudiwika	Chizindikiritso 1	Mapu oletsa
Kudiwika	Chizindikiritso 2	Chingwe Change
Mayeso	pH	Njira 0631 ya Chip 2020
	Kukhala Uliri	Kukwera Kwambiri kwamadzimadzi a chromatography (HPLC)
	Otsalira E.Coli amakhala ndi protein protein	Elia
	Otsalira E.Coli DNA	Q - PCR
	Otsalira e.coli rna	Q - PCR
	Zotsalira za maantibayotiki	Elia
	Endottoxin	Njira 1143 ya Chip 2020
	Kusamutsa	Njira 1101 ya Chip 2020
Kukhazikika kwamphamvu	DNA kuweta	Njira 0401 ya Chip 2020

* Dziwani: Mapiri okhazikitsidwa qc ofananira ndi nsanja zosiyana zaukadaulo, ndi njira za QC kuphatikiza koma osangokhala pamwamba pa zinthu.

Project Projekine

Dongosolo Loyang'anira Ntchito

Gulu la Phill Hillgene

Kutsitsi

Kuphatikiza njira za CDMO yagalimoto - T

Kafukufuku wanu sangathe kudikirira - Ngakhale anu sayenera kupereka!

Blash BlueKit Kit amapulumutsa:

✓ Lab - Kulondola

Kutumiza Kutumiza Padziko Lonse

✓ 24/7 Chithandizo