Virur transiction Sohancer A / B / C (Ro / GMP)
Virur transiction Sohancer A / B / C (Ro / GMP)
✔ Zero cytotoxicity (Vualicality> 95% Post - Chithandizo)
✔ Zimaphatikizapo cell - ma protocols enieni (T / nk / hsc oyenera)
Mwachidule:
BuluuniVirul E - Hancer ™ ndi gulu la okwera - kugwira ntchito, yopanda - kutsegula kwa cytotoxic kwa vetoneral vector. Kubwezera a Njira Zapadera Zochita, imagwiritsidwa ntchito kwambiri ku Virul Inction Maselo a T, maselo a NK, ndi HSCS.
Virul E - Hancer ™ Kachita - cell membrane fusision ndi kunyamula katundu (kuchokera ku plasma membrane ku nyukiliya) ndi:
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Kuchepetsa mafayilo a membrane
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Kulimbikitsa lipid kusinthana & transmerane kugwirizanitsa
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Kutulutsa mopitilira muyeso
Mapulogalamu
✔ Kumathandiza pakusintha kwa Ma cell a NK, TLASS, HSCS, ndi zina zolimba - kwa ma cell transduc
✔ ogwirizana Vsvg - ndi baev - pseudotyled Lentiviruses / retroviruses
✔ Amachepetsa mphamvu ya ma virus (Mtengo - Kupulumutsa)Mawonekedwe Ofunika
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Kukula kwa ntchito yothandiza pa 2-10X -
Otsika mtengo pochepetsa kugwiritsa ntchito
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Amalimbikitsa chitetezo: Amawongolera nambala ya viral Copy (VCN) kuti mutsatire FDA / EMA / NMMA kachitidwe kazinthu
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Zosankha Zosintha: Kupezeka Ruo (kafukufuku wogwiritsira ntchito okha) ndi gmp kwa R & D kwa malonda - kupanga
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Kuphatikizidwa kosakira: Ma protocol othamangitsidwa pama cell osiyanasiyana, onetsetsani kuti kulumikizana ndi makope ambiri omwe alipo
Kutsegula kwa Lentivilcycyction ya T maselo a T E - Hancer a + b
Tchulukitsa T - Kutumiza kwa maselo: Viral E - Hancer A + B Slants Misika (Kumanzere) Galimoto - T maselo: Virul E - Hancer a + B chithandizo,(Kumanja) Galimoto - Maselo a T: Wowongolera (Moi = 0,5)
Kupititsa patsogolo kwa Lentivil Transction mankhwala kwa NK ndi Virul E - Hancer C
Kusanthula kofananira kwa maselo a NK Cell Tembenukidwe Kumanzere: Kutulutsa cytometry ya I15 NK maselo omwe amathandizidwa ndi virul E - Hancer c
: Viral E - Hancer c vs. opikisana (moi = 1) kumanja: Kutulutsa cytometry ya i15 nk maselo (osasinthika)
Kupititsa patsogolo kwa retrovil thiration mu ma cell ndi virus e - Hancer c
Kusanthula kwa T - Kutulutsa kwa cell (A) Galimoto - ma cells omwe amapangidwa ndi Virul E - Hancer c,(B) Galimoto - Ty ma cell (owongolera osavomerezeka)
: Viral E - Hancer c vs opikisana (moi = 5)
Onjezani Kutulutsa kwa Hintivilction kwa HSCES pogwiritsa ntchito viral E - Hancer A + D Kuphatikizana
Kusanthula kofananira kwa mphamvu ya HSS kutumizidwa: Virul E - Hancer A + D vs. Misika ina (Moi = 5)
| Nambala | Dzinalo la zolaula | Kuchuluka (mwachitsanzo. - Gighte) |
| 1 | Kachilombo | Pofunika |
| 2 | Chikhalidwe chakati | Pofunika |
| 3 | Zisanu ndi chimodzi - mbale | 1 |
| 4 | T25 Flask | 1 |
| 5 | T75 Flusk | 2 |
| 6 | T175 Flask | 2 |
| 7 | 15 ml centrifuge chubu chubu | Ambiri |
| 8 | 50 ml centrifuge chubu chubu | Ambiri |
| 9 | 10 ml pipette | Ambiri |
| 10 | 25 ml pipette | Ambiri |
| 11 | Malangizo a 1 ml | Ambiri |
| 12 | 200 μl pipette malangizo | Ambiri |
Kutumiza Zambiri
Timapereka kunyamula mayendedwe onse pamalamulo onse. Nthawi zambiri, oda yanu ifika mkati mwa 5 - masiku 7 a bizinesi ku United States komanso mkati mwa masiku 10 antchito a mayiko ena. Komabe, chonde dziwani kuti kupembedza kumidzi ingatenge nthawi yayitali.
Nthawi Yotumiza: Maodawo amakonzedwa mkati mwa 1 - masiku atatu antchito. Dongosolo lanu litatumizidwa, mudzalandira imelo yotsimikizira ndi chidziwitso chotsatira.
Chidziwitso chofunikira
Kukonzanso: Kulamula kwaperekedwa, nyumba yathu yosungirako ikusowa nthawi kuti akonze dongosolo lanu. Mudzalandira chidziwitso kamodzi oda yanu yatumizidwa.
Nthawi zoperekera: Nthawi zambiri, phukusi lidzaperekedwa mkati mwa nthawi yomwe ikuchitika. Komabe, tsiku lenileni la zoperekera lingakhudzidwe ndi makonzedwe, nyengo, ndi zinthu zina zakunja. Nthawi yoperekera idzakhala yotalikirapo kuposa masiku onse okhudzana ndi malo ophatikizika kapena zinthu zosinthidwa. Chonde onani chidziwitso chotsatira tsiku lolondola kwambiri.
Nkhani Zotumiza: Ngati mukuwona kuti phukusi lanu silinaperekedwe mkati mwa nthawi yodziwika; Zambiri zotsatila zikuwonetsa kuti phukusi laperekedwa koma simunalandire; kapena phukusi lanu limaphatikizapo zinthu zosowa kapena zolakwika kapena zovuta zina, chonde lemberani kasitomala mkati mwa masiku 7 a tsiku lolipira kuti tithe kuthana ndi mavutowa mwachangu.
Kutsatira kutsatira
Dongosolo lanu litatumizidwa, mudzalandira imelo ndi nambala yolondola komanso ulalo kuti muthe kutumiza.
Mutha kutsatanso dongosolo lanu mwachindunji patsamba lathu podula muakaunti yanu ndikuwona mbiri yanu.
Zoletsa Kutumiza
Chonde lembani adilesi yamsewu mwatsatanetsatane, osati bokosi kapena adilesi yankhondo (apo). Kupanda kutero, tiyenera kugwiritsa ntchito EMS kuti tibweretsenso ena (amayamba pang'onopang'ono kuposa ena, kutenga 1 - miyezi iwiri kapena kupitilira).
Ntchito Zogwirira Ntchito ndi Msonkho
Chonde dziwani kuti ntchito zilizonse zamitundu, misonkho, kapena zolipirira zobwereketsa zomwe zikugulitsidwa ndi udindo wa wogula. Zolipira izi zimasiyana kutengera dzikolo ndipo zimatsimikizika ndi akuluakulu akwanuko am'deralo.
Pogula kuchokera patsamba lathu, mukuvomera kulipira ntchito kapena misonkho yomwe imagwirizana ndi dongosolo lanu. Sitili ndi vuto la kuchedwa chifukwa cha chilolezo cha miyambo.
MALANGIZO OTHANDIZA
Dongosolo lanu litafika pamalo osankhidwa kapena malo operekera, chonde onetsetsani kuti ikutola. Ngati phukusi silinatengedwe mkati mwa nthawi yomwe yasankhidwa, tidzakutumizirani chikumbutso kudzera pa imelo kapena SMS. Komabe, ngati phukusi silikusonkhanitsidwa nthawi yayitali, ndipo kutayika kapena kuwonongeka kulikonse kumachitika chifukwa, wogula azikhala ndi mlandu. Tikukumbutsani mokoma kuti mutole phukusi lanu mwachangu kupewa nkhani zilizonse zomwe zingachitike.
Chidziwitso: Pamene malonda athu amagwera pansi pa gulu lapadera, amabwerera ndi kubweza sikuvomerezedwa.
