Nā lawelaweʻo CDMO no Plasmids - It Scrift / N non?
Nā lawelawe
| Nā lawelawe CDMO no nā plasmids | ||||
| Nāʻano | Nā lawelawe | |||
| Iit papa | 1 | Ua kūkulu kūʻokoʻa iʻehā - Nā'ōnaehana plasmid |
● KOLOHE LOA'LA BOBLAN BETLANT BACHS - GECOMS System ● Kanamycin - ke kū'ēʻana ●ʻAʻohe laikini Passense |
● Hoʻopili i nā mea seamless e hoʻouna i ka hoʻounaʻana ● GMP - E like me nā papa hana ● GMP - E like me ka'ōnaehana hoʻokele maikaʻi ● Hoʻomohala a me nā palapala hōʻoia |
| 2 | ʻO ka hoʻokumuʻana o nā waihona kālā (GMP - E like me) |
● helu helu a me ka kiko'ī o nā waihona kālā e hanaʻia |
||
| 3 | Okami hana a me ka ho'āʻoʻana (gm - e like me) |
● Hoʻomoe i ka hana hana hana a me ka kiko'ī |
||
Loaʻa
| Loaʻa i kā mākou'ōnaehana PLASSMID:
• kahi paʻa kūʻokoʻaʻehā -ʻO ka'ōnaehana plasmad me Kanamycin -ʻO Cumist Guge • kahi'ōnaehana me ka hiki ke hiki i ka maikaʻi • nā mea iʻikeʻia, nā mea iʻikeʻia, hoʻopiʻi me nā koi, a me ka pono •ʻO kaʻike nui ma nā hoʻouna i hoʻopaʻaʻia • Ke kaʻa - T mau mea e koho ai i ka hoʻohanaʻana i ka hoʻohanaʻana i ka hoʻohanaʻana i ka hoʻohanaʻana a me ka hoʻohanaʻana • 2 - 5 BUT BUT BUCKS kiʻekiʻe ma hope o ka hoʻohanaʻana i ka'ōnaehana plassmid mai ka hoʻohālikelikeʻana i kekahi mau papahana |
Loaʻa i kā mākou hana hana. • ka manuahi o nā antibiotics a pau i ka hana hana • ka hana hoʻolālā a me ka hana kālā ma nā papa hanaʻokoʻa • Hoʻopiha piha ma waena o nā wahiʻole o ka sterile a me nā wahi sterile • e hoʻokaʻawale i nā huahana hope e hoʻohana ana i kahi isollator • E hoʻopau i nā Doss CTD no ka paleʻana i ka plasmid (no ka vector hope loa eʻaeʻia e 3 - 4 mau mahina o kekahi mau huahana |
Hana hana hana
Honua mālamalama
| Mea hōʻike | Kūlana hōʻike | |
| Helehelena | Nānā nānā | |
| Maopopo | KAUKU 1 | ʻO ka paleʻana |
| Maopopo 2 | Sanger Senging | |
| Hōʻailona | pH | Kaʻina 0631 o Chp 2020 |
| ʻO Menu ka hoʻomaʻemaʻe | ʻO ka hana nui o ke chrisotography (HPLC) | |
| Ka nohoʻana e.cooli host protein | Elisa | |
| Nā Kūlana Echalli DNA | Q - PCR | |
| Nā Kahu Echali RNA | Q - PCR | |
|
ʻO nā antibiotics |
Elisa | |
| Endotoxin | Kaʻina 1143 o Chp 2020 | |
| Huakai | Kaʻina 1101 o Chp 2020 | |
| Ka manaʻo noʻonoʻo | Dna lete | Ka Waihona 0401 o CHP 2020 |
Papahana hana
Hoʻolālā pāʻoihana hana
ʻO ka huiʻoihana hoʻokele kelepona,ʻo ia ka hana o nāʻepekema nui, nā limahana hana, e hana pono iā TA A me nā hana GMP.
