ʻO Viral transducing Enhancer A / B / C (Rou / GMP)
ʻO Viral transducing Enhancer A / B / C (Rou / GMP)
✔ Zerotikani (vierliility> 95% Post -
✔ Hoʻohui i nā cell - nā protocols kūikawā (T / NK / HSC Old)
Kailima:
UliuliViral E - he papa o High - Efficiencycyy, non - cytotoxic transduces no nā mea hoʻolimalima a me nā retroviral. Leveraging a ʻO ka hana likeʻole o ka hana, ua kūpono loa ia no ka hoʻololiʻana i ka maʻi viral i loko TEELS, NK Celells, a me HSCS.
ʻO Vi Caral e -ʻO Doncer ™ Endhaces ʻO ka wahine - kelepona mebrane cusion a me nā lawe kaua intracellular (mai plasma membrane i ka nucleus)
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E hōʻemi ana i nā membrane membrane
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Ke hoʻolauleʻa nei i nā hoʻololi lipoid & transmemmrane trafficing
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ʻO ka hoʻololiʻana i ka hōʻike profile stein
Noi
✔ Hoʻonui i ka hana consduction transduction no ka Nk cells, t cells, hscs,
✔ Hoʻohālikelike me Vsvg - a me ka baev - pseudotyped lentivissury / retrovires
✔ E hōʻemi i nā koina o nā koiUku - Mālama)Nā hiʻohiʻona nui
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Hoʻonui i nā mea hoʻokūkū transduction e 2-10x -
Nā kumukūʻai haʻahaʻa haʻahaʻa Ma ka hōʻemiʻana i ka hoʻohanaʻana i ka hoʻohanaʻana
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E hoʻomau i ka palekana: E hūnā i ka helu kope kope kope (VCN) e hoʻokō me FDA / EMA / NMPA Koisali
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Nā Koho Kākoʻo LLE: Loaʻa i loko Ruo (hoʻohana noiʻi wale nō) a me GMP Grades no ka r & d i ka hale kūʻai - scalect scation
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Hoʻohui i ka hoʻohuiʻana: Optimized Protocols no nāʻano kelepona paʻa, e hōʻoia i ka hoʻohālikelikeʻana me nā hana hana
Hoʻonuiʻo Lentivaral transduction i nā cell e t cell e shral e - Hancer a + b
Ma mua o T -ʻO ka consud consunction (Hema) Kaʻa - t cell: viral e - adcer a + b ka mālama,(Akau) Nā kaʻa - T CERLL
Hoʻonui i ka hoʻololi o ka mea hoʻolimalima i nā cell cell ma viral e - Hancer C
Hoʻohālikelike i ka loiloi o NK Cell Consduble i ka hana i koe: e kahe ana i ka cytometry o i15 nn cell cell me viral e - harcer c
: Viral e - Hancer C Vs.
Hoʻonui i ka transduction retrovil i nā cell e t cell e ka viral e - Hancer C
Hoʻohālikelike hoʻohālikelike o t - call transduces (A) Kaʻa kaʻa - Ua weheʻia nā cell Kokua e - Hancer C,(B) Kaʻa - t mau cell (control control)
: Viral e - Hancer C Vs. Sticators (Moi = 5)
Hoʻopili i ka hoʻololiʻana o ka lentivinural i nā hscs e hoʻohana ana i viral e - Hancer A + D hui pū
Hoʻohālikelike Hoʻohālikelike o HSC Consduces Effudiency: He viral e - Hancer a + D vs.
| Ka Uila | ʻO ka inoa o nā mea hoʻoponopono hou | Ka nui (eg. Eono - maikaʻi maikaʻi) |
| 1 | Kaukini | E like me ka mea e pono ai |
| 2 | ʻAno moʻomeheu | E like me ka mea e pono ai |
| 3 | ʻEono - Plate maikaʻi | 1 |
| 4 | T25 Flask | 1 |
| 5 | T75 Flask | 2 |
| 6 | T175 FlaSK | 2 |
| 7 | 15 ml centitrifuge tube | He mau nui |
| 8 | 50 ml centifuge tube | He mau nui |
| 9 | 10 ml pipette | He mau nui |
| 10 | 25 ml pipette | He mau nui |
| 11 | 1 ML Pipette mau'ōlelo aʻoaʻo | He mau nui |
| 12 | 200 μl kipertte aʻoaʻo | He mau nui |
ʻO kaʻike moku
Hāʻawi mākou i nā mea i hoʻokaʻawaleʻia ma nā kauoha a pau. ʻO ka maʻamau, e hiki mai kāu kauoha i loko o 5 - 7 mau lāʻoihana maʻAmelika Hui PūʻIa a ma loko o 10 mau lāʻoihana. Eia nō naʻe, eʻoluʻolu e hoʻomaopopo i ka laweʻana i nā wahi'āina e lōʻihi lōʻihi paha.
ʻO ka manawa hoʻouna: Ua hana pinepineʻia nā kauoha i loko o 1 - 3 mau lāʻoihana. I ka manawa i hoʻounaʻia ai kāu kauoha, e loaʻa iāʻoe kahi leka uila hōʻoia me kaʻikeʻike.
ʻO kaʻike nui
E kauoha i ke kauoha: Ma hope o ka ukuʻia o ke kauoha, pono kā mākou hale kūʻai aku i kekahi manawa e hana i kāu kauoha. E loaʻa iāʻoe kahi hoʻolaha i ka wā i hoʻounaʻia ai kāu kauoha.
Nā manawa hāʻawi: Ma ka hapa nui o nā hihia, e hāʻawiʻia ka pākeke i loko o ka manawa i kohoʻia. Eia nō naʻe,ʻo ka lā lawe maoli e pili ana i ka holoʻana e nā hoʻonohonoho o ka holo, nā kūlana o ka wā, a me nā mea'ē aʻe. E lōʻihi ka lōʻihi o ka hana o ka hāʻawiʻana ma mua o nā kauoha no nā kauoha e komo i nā mea ma mua a iʻole nā mea maʻamau. Eʻoluʻolu e kiʻi i kaʻikeʻike no ka lā hoʻokele nui loa.
Nā pilikia hoʻouna: Ināʻikeʻoeʻaʻole i hāʻawiʻia kāu pākeke i loko o ka manawa i'ōleloʻia; Hōʻike kaʻikeʻikepili i hōʻikeʻia ua hāʻawiʻia ka pākeke akāʻaʻoleʻoe i loaʻa; a iʻole kāu pākeke e pili ana i nā mea i hala a iʻole nā mea kūponoʻole a iʻole nā pilikia logistics, eʻoluʻolu e hoʻopili i kā mākou lawelawe kūʻai aku i loko o 7 mau lā e hiki ai iā mākou ke helu koke i kēia mau pilikia.
Kauoha i ka huliʻana
Ke hoʻounaʻia nei kāu kauoha, e loaʻa iāʻoe kahi leka uila me kahi helu helu a me kahi loulou e nānā i kāu hoʻouna.
Hiki iāʻoe ke nānā pololei i kāu kauoha ma kā mākou pūnaewele ma o ka hoʻopaʻaʻana i kāu moʻokāki a nānā i kāu mōʻaukala kauoha.
Nā palena hoʻouna
Eʻoluʻolu e hoʻopiha i ka leka uila i ka kiko'ī Ināʻaʻole, makemake mākou e hoʻohana iā EMS no ka hāʻawiʻana (ʻoi aku ka lohi ma mua o nā mea'ē aʻe, e lawe ana i kahi 1 - 2 mau mahina a lōʻihi paha).
Nā hana maʻamau a me nā kulekeleʻauhau
E hoʻomaopopo eʻaeʻia kekahi mau hana maʻamau,ʻauhau, a iʻole nā uku hoʻolimalima a i hoʻopuka i ka wā o ka hoʻounaʻana i ka mea kūʻai. ʻO kēia mau uku e hāʻawiʻia ma muli o ka'āina kahi i hāʻawiʻia a hoʻoholoʻia e nā luna maʻamau o nā kūloko.
Ma ke kūʻaiʻana mai i kā mākou pūnaewele, eʻaeʻoe e uku i nā hana a iʻole nāʻauhau e pili ana i kāu kauoha. ʻAʻole mākou e kuleana no nā delays i hoʻokumuʻia e nā hoʻomaʻemaʻe maʻamau.
Nā kulekele koho koho
Ke hōʻea nei kāu kauoha i ka wahi koho i kohoʻia a iʻole ka hāʻawiʻana i kahi wahi, eʻoluʻolu e hōʻoia i ka hōʻiliʻili wikiwiki. Ināʻaʻole i kiʻiʻia ka uku i loko o ka manawa i kohoʻia, e hoʻouna mākou i kahi hoʻomanaʻo ma o ka leka uila a iʻole SMS. Eia naʻe, ināʻaʻole i hōʻiliʻiliʻia ka pākaukau i loko o ka manawa i kohoʻia, a loaʻa paha kekahi mau maʻi a iʻole ka maʻi e like me ka hopena, e mālamaʻia ka mea kūʻai aku. Mahalo nui mākou iāʻoe e hōʻiliʻili i kāu uku wikiwiki e pale i nā pilikia kūpono.
MEA KAHAKAU: E like me kā mākou huahana i lalo o kaʻano kūikawā
