RCL Detection: VSVG Gene Copy Number Kit | BlueKit

RCL Detection: VSVG Gene Copy Number Kit | BlueKit

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In the rapidly evolving landscape of genetic research and molecular diagnostics, the quest for precision, efficiency, and reliability in detecting recombinant lentivirus (RCL) has led to the development of state-of-the-art tools and methodologies. BlueKit is at the forefront of this innovation, presenting the RCL(VSVG) Gene Copy Number Detection Kit designed for quantitative Polymerase Chain Reaction (qPCR) applications. This kit represents a pivotal advancement in the field of gene therapy, viral vector manufacturing, and biosafety testing, offering researchers an unparalleled tool for the accurate quantification of RCL.

 

 

Standard curve

 

 

 

 

Datasheet

 



The RCL(VSVG) Gene Copy Number Detection Kit harnesses the power of qPCR technology, a revolutionary method known for its sensitivity, specificity, and speed in gene expression analysis. The kit is meticulously designed to target the VSV-G gene, a common envelope protein gene used in pseudotyping lentiviral vectors, which facilitates a broad range of cell type transduction. By focusing on this gene, the kit provides a direct, quantifiable measure of RCL presence, essential for ensuring the safety and efficacy of lentiviral vector-based gene therapy products. Embarking on the journey of RCL detection with the BlueKit’s RCL(VSVG) Gene Copy Number Detection Kit not only elevates the standard of research but also ensures compliance with regulatory standards for gene therapy products. The kit includes a comprehensive set of reagents and a clearly outlined standard curve for quantification, simplifying the process of RCL detection. Through its precision, the kit aids researchers in distinguishing between background noise and actual RCL presence, a critical aspect in the quality control of lentiviral preparations. With its robust design and ease of use, the kit is an indispensable tool for researchers dedicated to advancing the field of gene therapy, ensuring that groundbreaking treatments can be developed safely and effectively.
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Overview
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Cat.No. HG-RC001                                                       $1,508.00

This kit is designed for the quantitative detection of RCL gene copy number in the genome of CAR-T
cells prepared by using HIV-1 lentiviral vector technology.
 
This kit adopts the fluorescent probe method and multiplex PCR method to detect the DNA sequence related to integration or expression function on the transfer plasmid, and the VSVG gene copy number in the sample can be calculated. The kit is a rapid, specific and reliable device.


Performance

Assay range

  • 1.00×101~1.00×105copies/μL

 

Limit of quantitation

  • 8 copies/μL

 

Limit of detection

  • 1 copies/μL

 

Precision

  • CV%≤15%


Instructions for Use of RCL (VSVG) Gene Copy Number Detection Kit (qPCR) RCL(VSVG) Gene Copy Number Detection Kit-Datasheet
Inquire About This Product
FAQ
What are the precautions for using the product?
What are the precautions for using the product?
The kit is intended for scientific research only
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