Chingwe chokhazikika cha cell protein chotsalira cha 293t
Chingwe chokhazikika cha cell protein chotsalira cha 293t
✔ Pre - Zithunzi zokutira Sungani maola atatu vs. njira
✔ Zimaphatikizapo FDA 21 CFR Gawo 11 ma temlates
Mafotokozedwe Akatundu:
Sangweji ya Elisa yochulukirapo kupezeka kwa cell protein (HCP) zonyansa za bilogikic zopangidwa pogwiritsa ntchito mizere ya 293T. Imazindikira> 95% ya 293T hcp contzas monga momwe zimatsimikiziridwa ndi 2d - blot.
Zolemba:
|
Palamu |
Chifanizo |
|
Kuchuluka kwa kuchuluka |
37-27,000 ng / ml |
|
Choq |
37 ng / ml |
|
Chidule (intra - kuthamanga) |
Cv <10% |
|
Kulunjika |
85-115% Kubwezeretsa |
Zofunikira:
Kupeza kwathunthu: kumazindikira zonse zosungunuka komanso zopanda nzeru 293T HCPS
GMP - Zogwirizana: Kumanani ndi Ich Q6B Zofunikira
Zokhazikika: Zophatikiza zisanachitike - Zilikidwe
Curve Standard:
Tsamba lazambiri:
| Chipangizo | Chifanizo | Malangizo |
| 293T hcp yophika mbale | 8 Wells × 12 imakwera × 1 | Wokonzeka - - Gwiritsani Ntchito |
| Biot Inter - 293T hcp antibody | 150 μL × 1 vial | Kuchepetsa 1:99 ndi buffer |
| Streptavidin - HRP Conjugate | 375 μl × 3 Mbale | Kuchepetsa 1: 9 ndi chopukusira |
| 293T HCP Standard (81 μg / ml) | 600 μL × 1 vial | Kutsatira njira yosilira |
| Kuwuma | 1 g × 1 vial | Lowani mu 100 ml 1 × pbs - T (1G / 100ml) |
| 10 × Pbs - T Kutsuka Buffer | 50 ml × 1 botolo | Kuchepetsa 1: 9 ndi madzi owoneka bwino |
| TMB gawo | 15 ml × 1 botolo | Wokonzeka - - Gwiritsani Ntchito |
| Siyani yankho | 15 ml × 1 botolo | Wokonzeka - - Gwiritsani Ntchito |
| Plate panja | 1 chidutswa | Wokonzeka - - Gwiritsani Ntchito |
| Buku la malangizo | 1 kope | - |
chifanizo
| Mtundu | 37 - 27,000 ng / ml |
| Malire a kuchuluka (Loq) | 37 ng / ml |
| Chidule | CV ≤ 10% |
| Kulunjika | Re ≤ ± 15% |
| KusungaKutentha | 4 ℃ |
| Kulembana | 96 Chabwino |
| Lodu | 30 ng / ml |
Kutumiza Zambiri
Timapereka kunyamula mayendedwe onse pamalamulo onse. Nthawi zambiri, oda yanu ifika mkati mwa 5 - masiku 7 a bizinesi ku United States komanso mkati mwa masiku 10 antchito a mayiko ena. Komabe, chonde dziwani kuti kupembedza kumidzi ingatenge nthawi yayitali.
Nthawi Yotumiza: Maodawo amakonzedwa mkati mwa 1 - masiku atatu antchito. Dongosolo lanu litatumizidwa, mudzalandira imelo yotsimikizira ndi chidziwitso chotsatira.
Chidziwitso chofunikira
Kukonzanso: Kulamula kwaperekedwa, nyumba yathu yosungirako ikusowa nthawi kuti akonze dongosolo lanu. Mudzalandira chidziwitso kamodzi oda yanu yatumizidwa.
Nthawi zoperekera: Nthawi zambiri, phukusi lidzaperekedwa mkati mwa nthawi yomwe ikuchitika. Komabe, tsiku lenileni la zoperekera lingakhudzidwe ndi makonzedwe, nyengo, ndi zinthu zina zakunja. Nthawi yoperekera idzakhala yotalikirapo kuposa masiku onse okhudzana ndi malo ophatikizika kapena zinthu zosinthidwa. Chonde onani chidziwitso chotsatira tsiku lolondola kwambiri.
Nkhani Zotumiza: Ngati mukuwona kuti phukusi lanu silinaperekedwe mkati mwa nthawi yodziwika; Zambiri zotsatila zikuwonetsa kuti phukusi laperekedwa koma simunalandire; kapena phukusi lanu limaphatikizapo zinthu zosowa kapena zolakwika kapena zovuta zina, chonde lemberani kasitomala mkati mwa masiku 7 a tsiku lolipira kuti tithe kuthana ndi mavutowa mwachangu.
Kutsatira kutsatira
Dongosolo lanu litatumizidwa, mudzalandira imelo ndi nambala yolondola komanso ulalo kuti muthe kutumiza.
Mutha kutsatanso dongosolo lanu mwachindunji patsamba lathu podula muakaunti yanu ndikuwona mbiri yanu.
Zoletsa Kutumiza
Chonde lembani adilesi yamsewu mwatsatanetsatane, osati bokosi kapena adilesi yankhondo (apo). Kupanda kutero, tiyenera kugwiritsa ntchito EMS kuti tibweretsenso ena (amayamba pang'onopang'ono kuposa ena, kutenga 1 - miyezi iwiri kapena kupitilira).
Ntchito Zogwirira Ntchito ndi Msonkho
Chonde dziwani kuti ntchito zilizonse zamitundu, misonkho, kapena zolipirira zobwereketsa zomwe zikugulitsidwa ndi udindo wa wogula. Zolipira izi zimasiyana kutengera dzikolo ndipo zimatsimikizika ndi akuluakulu akwanuko am'deralo.
Pogula kuchokera patsamba lathu, mukuvomera kulipira ntchito kapena misonkho yomwe imagwirizana ndi dongosolo lanu. Sitili ndi vuto la kuchedwa chifukwa cha chilolezo cha miyambo.
MALANGIZO OTHANDIZA
Dongosolo lanu litafika pamalo osankhidwa kapena malo operekera, chonde onetsetsani kuti ikutola. Ngati phukusi silinatengedwe mkati mwa nthawi yomwe yasankhidwa, tidzakutumizirani chikumbutso kudzera pa imelo kapena SMS. Komabe, ngati phukusi silikusonkhanitsidwa nthawi yayitali, ndipo kutayika kapena kuwonongeka kulikonse kumachitika chifukwa, wogula azikhala ndi mlandu. Tikukumbutsani mokoma kuti mutole phukusi lanu mwachangu kupewa nkhani zilizonse zomwe zingachitike.
Chidziwitso: Pamene malonda athu amagwera pansi pa gulu lapadera, amabwerera ndi kubweza sikuvomerezedwa.
