NK and TIL Cell Expansion Reagents（K562 Feeder Cell)
NK and TIL Cell Expansion Reagents（K562 Feeder Cell)
✔ Pre-irradiated, multi-cytokine enhanced for superior NK/TIL expansion.
✔ Validated for both cord blood and PBMC sources - CAR-ready.
✔ Pre-qualified for IND submissions with full characterization data.
Overview:
The NK/TIL Expansion Reagent consists of engineered, irradiated K562 feeder cells expressing IL-21 and other key cytokines. When combined with standard immune cell media, this system enables:
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High-efficiency expansion of NK cells and TILs from PBMCs or cord blood -
High-purity populations (>90% target cells)
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Rapid, scalable activation under optimized cytokine signaling
Key Applications
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Activation & expansion of NK cells, CAR-NKs, and TILs
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Process development: Early-stage optimization of cell therapy workflows
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GMP manufacturing: Supports IND-enabling studies and post-approval process changes
Regulatory & Performance Benefits
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GMP-compliant formulation
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Consistent, high-yield expansion (up to 1000-fold)
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Pre-validated for clinical use
Datasheet
NK Cell Expansion Efficiency and Viability Across Multiple Donors
Donor-to-Donor Variability in NK Cell Purity
Enhancement Effect of Viral E-hancerC on Lentiviral Transduction in NK Cells
NK Cell Transduction: Viral E-hancerC Outperforms Competitors at MOI 1 Left panel: Flow cytometry of IL-15 NK cells with Viral E-hancerC
Right panel: Flow cytometry of IL-15 NK cells without transduction enhancer
Viral E-hancerC vs. competitors: T-cell transduction at MOI 5 Left: Flow cytometry of CAR-T cells with Viral E-hancerC
Right: Flow cytometry of CAR-T cells without transduction enhancer
Enhancement effect of Viral E-hancer A + Viral E-hancer D combination on lentiviral transduction in HSCs
Comparative analysis of HSC transduction efficiency: Viral E-hancerA + Viral E-hancerD combination versus competitors (MOI=5)
| Serial number | Name of reagentand consumables | Dosage (for ~1-2E6 PBMC initiation) |
| 1 | Serum-free medium | 2 L |
| 2 | IL-2 | 4 sticks (200,000 IU/stick) |
| 3 | 0.9% NaCl injection | ~500 mL |
| 4 | 20% human serum albumin | ~15 mL |
| 5 | Ficoll lymphocyte isolation solution | ~25 mL |
| 6 | NC-200 counting chamber (or equivalent) | As needed |
| 7 | 6-well plate | 1 |
| 8 | T25 flask | 1 |
| 9 | T75 flask | 1 |
| 10 | T175 flask | 2 |
| 11 | 640 cm² cell culture bag | 2 |
| 12 | 15 mL centrifuge tube | 1 |
| 13 | 1.5 mL centrifuge tube | As needed |
| 14 | 10 mL / 25 mL pipettes | As needed |
| 15 | 1 mL / 200 μL pipette tips | As needed |
Specification
| Performance Parameter | Specification |
| NK/CAR-NK Expansion Fold | >5,000× (14 days) |
| Cell Viability | ≥90% |
| Cell Purity (CD56⁺/CD3⁻) | >95% |
Shipping Information
We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.
Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.
Important Information
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Shipping Restrictions
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Note:As our product falls under the special category, returns and refunds are not accepted.
