Lentivirus Titer p24 Rapid ELISA Detection Kit
Lentivirus Titer p24 Rapid ELISA Detection Kit
✔ Correlates with TU/mL (1 ng p24 ≈ 1×10<sup>4</sup>–10<sup>5</sup> transducing units)
✔ 21 CFR Part 11-ready electronic reporting templates included
✔ Spike recovery 92-108% in purified LV samples
Overview:
The kit employs a sandwich ELISA methodology for HIV-1 p24 quantification:
Microplate coated with anti-p24 monoclonal antibody
Forms antibody-antigen-detection antibody complex during RT incubation
TMB substrate produces colorimetric signal proportional to p24 concentration
Performance Specifications:
Parameter |
Specification |
Dynamic Range |
1.37–1000 ng/mL |
Sensitivity (LoD) |
0.35 ng/mL |
Precision |
Intra-assay CV <10% |
Accuracy |
85–115% Recovery |
Key Features:
Rapid 45-min protocol (RT incubation)
Ready-to-use reagents
Correlates with viral titer (1 ng p24 ≈ 1×10<sup>4</sup>–10<sup>5</sup> TU)
Standard curve:
Datasheet:
what is on the box?
Component | Specification | Storage Condition |
Coated Plate | 1 × 96 wells | Room temperature |
Anti-HIV-1 p24-HRP Conjugate | 6 mL | 2-8°C |
HIV-1 p24 Standard Set | S0-S7 (7 standards + blank) | 2-8°C |
Virus Lysis Buffer | 6 mL | 2-8°C |
Sample Diluent Buffer | 50 mL | Room temperature |
10× PBST Wash Buffer | 50 mL | Room temperature |
TMB Substrate | 12 mL | 2-8°C |
Stop Solution | 12 mL | Room temperature |
Plate Sealers | 5 | Room temperature |
Instruction Manual | 1 | N/A |
specification
Assay range |
1.37-1000 ng/mL |
Detection sensitivity |
0.35 ng/mL |
Precision |
CV% ≤ 10%, RE% ≤ ±15% |
Shipping Information
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Important Information
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