Human Residual DNA Fragment Analysis Detection Kit（qPCR）
Human Residual DNA Fragment Analysis Detection Kit（qPCR）
✔Multiplex qPCR quantifies 99/200/307 bp fragments simultaneously.
✔NIST-traceable standards ensure metrological DNA quantification accuracy.
✔Validated 3-10⁵ fg/μL range meets ICH Q6B.
This kit is designed for the quantitative detection of the size distribution of Human residual hostcell DNA fragments in intermediates, semi-finished and finished products of various biologicalproducts.
This kit adopts the principle of PCR fluorescent probe method to quantitatively detect the sizedistribution of human residual host cell DNA fragments in the sample. The kit features threedifferent amplified fragments (99 bp, 200 bp and 307 bp), and the Human DNA quantificationreference is used to make standard curves for different amplified fragments respectively, andthe fragment distribution of Human residual DNA in the sample is analyzed through the ratio ofdifferent sizes of fragments.
This kit is a rapid, specific and reliable device, with the minimum detection limit reaching fg level.
Residual DNA fragment (> 99bp) Detection
Residual DNA fragment(>200bp) Detection
Residual DNAfragment(>307bp) Detection
| No. | Component | Fill Volume | Quantity | Storage Conditions |
| 1 | Human Primer & Probe Mix-99 | 300 μL | 1 | -18°C, protected from light |
| 2 | Human Primer & Probe Mix-200 | 300 μL | 1 | -18°C, protected from light |
| 3 | Human Primer & Probe Mix-307 | 300 μL | 1 | -18°C, protected from light |
| 4 | Human DNA Quantitative Reference | 60 μL | 1 | -18°C |
| 5 | 2× Probe qPCR Master Mix | 1.25 mL | 4 | -18°C |
| 6 | DNA Diluent | 1 mL | 4 | -18°C |
| 7 | IPC Mix | 450 μL | 1 | -18°C, protected from light |
| 8 | ROX High* | 200 μL | 1 | -18°C, protected from light |
| 9 | ROX Low* | 200 μL | 1 | -18°C, protected from light |
specification
| Parameter | Specification |
| Catalog Number | ic-HFool |
| Assay Range | 3.00×10¹ - 3.00×10⁶ fg/μL |
| Limit of Detection | 3.00×10⁰ fg/μL |
| Limit of Quantitation | 8 fg/μL |
| Kit Size | 300 Reactions |
| Storage Temperature | -20°C |
| Precision | CV% ≤ 15% |
Shipping Information
We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.
Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.
Important Information
Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.
Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.
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Shipping Restrictions
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Package Pickup Policy
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Note:As our product falls under the special category, returns and refunds are not accepted.
