Cell Cytotoxicity Assay Kit (Adherent Target Cells)
Cell Cytotoxicity Assay Kit (Adherent Target Cells)
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✔ CFSE-labeled target cells for clear identification
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✔ 7-AAD staining for precise dead cell detection
✔ Designed for adherent tumor cell lines
Overview:
This kit is designed to measure effector cell cytotoxicity against adherent target cells, suitable for NK cells, CAR-NK cells, CAR-T cells, TCR-T cells, and Tm cells in tumor-killing assays.
Kit Components:
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CFSE (Carboxyfluorescein Succinimidyl Ester, a membrane-permeable green fluorescent dye for target cell labeling)
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7-AAD (7-Aminoactinomycin D, a DNA-binding dye for dead cell detection)
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Medium B (Optimized for cytotoxicity assays)
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Assay Buffer (Ensures consistent assay conditions)
Key Applications
✔ Functional assessment of immune cell products (e.g., CAR-T, NK cells) against adherent tumor cell lines
✔ Process development & optimization in cell therapy research
✔ Release testing & stability studies for GMP-compliant manufacturing
Performance Features:
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Dual fluorescent labeling (CFSE for targets, 7-AAD for cytotoxicity)
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Standardized protocol for reproducible results
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Compatible with high-throughput systems (96/384-well formats)
Regulatory & Industry Compliance
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Validated for tumor cell lines (e.g., HeLa, A549, MCF-7)
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Suitable for IND-enabling studies (ICH Q2(R1) compliant)
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21 CFR Part 11-ready data reporting available
NK-mediated killing of CFSE+ A549 cells by flow cytometry
NK-mediated killing of CFSE+ solid tumor cells by flow cytometry
Datasheet:
| Mycoplasma Strain | Positive/Total | Closely Related Bacterial Strains | Positive/Total | Lactobacillus acidophilus | Streptococcus pneumoniae | Clostridium acetobutylicum |
|---|---|---|---|---|---|---|
| Mycoplasma orale | 24/24 | Mycoplasma arthritidis | 24/24 | Negative | Negative | Negative |
| Mycoplasma gallisepticum | 23/24 | Mycoplasma arginini | 24/24 | Negative | Negative | Negative |
| Acholeplasma laidlawii | 24/24 | Mycoplasma hyorhinis | 24/24 | Negative | Negative | Negative |
| Mycoplasma fermentans | 23/24 | Spiroplasma citri | 24/24 | Negative | Negative | Negative |
| Mycoplasma pneumoniae | 24/24 | Mycoplasma salivarium | 24/24 | Negative | Negative | Negative |
| Catalog No. | Component | Specification | Storage Temperature | Kit Position |
| HG-MD002 | Medium B | 100 mL | 2-8°C | Kit I |
| HG-FW001 | Assay Buffer | 100 mL | 2-8°C | - |
| HG-CFSE | CFSE | 1 vial | -20°C | Kit II |
| HG-7-AAD | 7-AAD | 1 vial | -20°C | - |
Specification
| Performance Parameter | Acceptance Criteria |
| Accuracy | 70–130% recovery |
| Robustness | 70–130% bias |
| Repeatability | CV < 10% |
| Intermediate Precision | CV ≤ 10% |
Shipping Information
We offer Refrigerated Transport on all orders. Typically, your order will arrive within 5-7 business days in the United States and within 10 business days for other countries. However, please note that delivery to rural areas may take a bit longer.
Shipping Time: Orders are typically processed within 1-3 business days. Once your order has been shipped, you’ll receive a confirmation email with tracking information.
Important Information
Order Processing: After the order has been paid, our warehouse needs some time to process your order. You will receive a notification once your order has been shipped.
Delivery Times: In most cases, the package will be delivered within the estimated time of arrival. However, the actual delivery date may be affected by flight arrangements, weather conditions, and other external factors. The delivery time frame will be longer than usual for orders that include preorder or customized items. Please refer to the tracking information for the most accurate delivery date.
Shipping Issues: If you find that your package has not been delivered within the specified time; the tracking information shows that the package has been delivered but you have not received it; or your package includes missing or incorrect items or other logistics issues, please contact our customer service within 7 days of the payment date so that we can address these issues promptly.
Shipping Restrictions
Please fill in the street address in detail, not PO BOX or military address (APO). Otherwise, we would have to use EMS for delivery (it's slower than others, taking around 1-2 months or even longer).
Customs Duties and Taxes Policy
Please note that any customs duties, taxes, or import fees incurred during shipping are the responsibility of the buyer. These charges vary depending on the destination country and are determined by local customs authorities.
By purchasing from our website, you agree to pay any applicable duties or taxes associated with your order. We are not responsible for delays caused by customs clearance.
Package Pickup Policy
Once your order has arrived at the designated pickup point or delivery location, please ensure prompt collection. If the package is not picked up within the designated time, we will send a reminder via email or SMS. However, if the package is not collected within the specified period, and any loss or damage occurs as a result, the buyer will be held responsible. We kindly remind you to collect your package promptly to avoid any potential issues.
Note:As our product falls under the special category, returns and refunds are not accepted.
