Ikirenga ADN ya DNA - Uburyo bwa Magnetic

Superior Genomic DNA Extraction Kit - Magnetic Bead Method

Ikirenga ADN ya DNA - Uburyo bwa Magnetic

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Mu bihe byose - Guhura Umwanya wa Biologiya ya Molecular, Gukuramo Hejuru - Ubwiza bwa Amoko ya Genomic butanga inzira kubisabwa byinshi byatangaga, harimo ariko ntibigarukira kuri PCR, gukurikiranwa, no gutandukana. Kumenya imiterere yubugome bwiyi nzira, Bluekit yishimiye kwerekana gukata - Amaraso / tissue / tissue / selile dna ya dna yo gukuramo dna, gukoresha uburyo bwiza bwa magnetic cyane. Ibicuruzwa bihagaze nkisezerano ryo kwitanga kwacu gutera imbere ubushakashatsi bwa siyansi dufasha gukuramo ADN hamwe nisuku itagaragara.

Porogaramu

Yerekana umusaruro mwinshi kandi usukuye hejuru ugereranije nibicuruzwa.

Electrophores muri 1% agarose kals

Kwambura No.1 & 2: Amaraso / tissue / selile dna yo gukuramo at (uburyo bwa magnetic

Umurongo No 3 & 4: ibikoresho bitumizwa mu mahanga

Ibisubizo byerekana ko ibice bibiri byakuweho ukoresheje ibikoresho by Bluekit® byuzuye nkibikoresho byatumijwe mu mahanga.

Kuramo ADN ya Newnation kuva mubisobanuro bibiri byamaraso hamwe nibikoresho byatumijwe mu mahanga hamwe n'ibikoresho bya Bluekit®, hanyuma ubyerekane hamwe na Nanodrop.

Ibisubizo byerekana ko ibikoresho by Bluekit® bifite 5 - 10% bitanga ibirenze ibikoresho byatumijwe mu mahanga.

Gushakisha ADN ya Genomic ya kalibre ndende isaba uburyo butagenda neza gusa ahubwo ko yizewe kandi umukoresha. Kit ya dna ya dna ya dna yateguwe neza kugirango yuzuze ibisabwa, kureba niba abashakashatsi bashobora kubona ADN bava mumaraso, tissue, na selile, na selile zoroshye ntagereranywa. Uburyo bwa magnetique bwerekana, imikorere yibanze mu bikorwa bya Kit, ikoresha ibice bya magneti kuri bind ADN ihitamo. Ibi byemerera kwihuta kandi byiyongereye byihuta bya ADN ya ginyabubi, bitangwa nabanduye nubururu bakunze kuvuga ubundi buryo bwo gukuramo. Nkigisubizo, ADN yakuyeho isuku, itunzwe neza na porogaramu nini y'ibinyabuzima. Byongeye kandi, ibikoresho byacu byerekana imikorere isumbabyo iyo ugereranije n'ibicuruzwa ku isoko. Binyuze mu guhitamo neza inzira yo gukuramo, ntabwo ari umusaruro wa ADN gusa ahubwo unareba ko ubunyangamugayo bwayo, bune cyane kandi kwizerwa kwubushakashatsi bwakurikiyeho. Waba urimo ukora genomen urukurikirane, kwishora mubikorwa bya pcr, cyangwa batange imitekerereze ya genetike, ibikoresho bya dna gendetic bitanga urufatiro rukomeye mubikorwa byawe bya siyansi bishobora gutera imbere. Hamwe nubururu, uzamure ubushakashatsi bwawe muburebure bushya, umutekano mubumenyi ushyigikiwe nibikoresho byiza siyanse igomba gutanga.

Umubare

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Shaka amagambo

Ongera ku Ikarita

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Incamake

Protocole

Ibisobanuro

Kohereza & kugaruka

Gufata amashusho

Injangwe.Ntabwo HG - Na100 $ 231.00

Ibi bikoresho byateguwe kubikurura byoroshye kandi neza bya genome. Ibi bikoresho birashobora gukoreshwagukuramo ingano ntoya yicyitegererezo nintoki kandi ikora murwego rwo hejuru - igipimo cyimitereremu buryo bwikora.

ADN ya Genomic yakuweho niki kikoresho irashobora gukoreshwa mugutabara ADN ya mbere ya ALC mubushakashatsi bumwe.