Txhim kho koj cov kev tshawb fawb nrog cov khau khiab DNA

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Txhim kho koj cov kev tshawb fawb nrog cov khau khiab DNA

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Hauv kev tshawb fawb nrawm nrawm ntawm kev tshawb fawb caj ces thiab kuaj sim, qhov kev xav tau txuag DNA cov hau kev extraction thiab txhim khu kev qha ntau dua. Cov kab mob xiav tau qhia peb cov kev txiav - ntaub / xov tooj Genomic hlaws qauv los tsim cov qauv tshiab hauv kev lag luam. Cov khoom no tau tsim tshwj xeeb los ua kom tau raws li cov kws tshawb fawb thiab cov kev ua si zoo tshaj plaws yog qhov ua tau zoo li DNO cov khoom siv thiab cov khoom lag luam tseem ceeb tshaj plaws thiab cov khoom sib tw. Firstly, lub pob kws ntawm peb cov khoom txoj kev vam meej yog nws cov txiaj ntsig ntau dua. Hauv txhua txoj kev extraction, peb cov khoom siv ua ke tau muab cov khoom siv DNA ntau dua, yooj yim ntawm cov kev tsom xam ntawm caj ces thiab kev sim. Txoj kev txhim kho no hauv cov txiaj ntsig tsis haum qhov tsis zoo; Ntawm qhov tsis sib xws, peb cov khoom siv tau ua kom muaj qib siab ntawm kev coj dawb huv. Cov khoom paug thiab cov neeg tsis muaj phom sij, tawm hauv qab DNA uas yog pristine thiab haum rau cov ntawv sau ua kom raug xws li pr, ua ntu zus, thiab tshuaj ntsuam xyuas SNP.

Cov Ntawv Thov

Qhia cov txiaj ntsig ntau dua thiab muaj kev coj dawb huv ntau dua rau cov khoom sib tw.

Electrophoresis hauv 1% agarose gels

Sawb No.1 & 2: Ntshav / ntaub / xov tooj Genomic DNA Extraction Cov Khoom Siv (Sib Nqus Hle Cov Qauv)

Sawb No.3 & 4: Cov Khoom Siv Hlau

Cov txiaj ntsig tau qhia tias cov khoom tawg ua yeeb yam tau muab cov khoom siv BlueKit® ua tiav raws li cov uas siv cov khoom siv hlau.

Extract genomic DNA los ntawm ob cov ntshav cov khoom siv thiab cov khoom siv BlueKit®, thiab tom qab ntawd pom cov kev xav nrog nanodrop.

Cov txiaj ntsig qhia tau hais tias Sebarkit® Cov khoom siv muaj 5 - 10% tawm los ntau dua li cov khoom siv txawv teb.

Peb cov tswv cuab cov qauv sib nqus hlaws qauv yog nyob ntawm lub siab ntawm cov txiaj ntsig zoo kawg. Cov txheej txheem no siv nyiaj minuscuetcuel beads uas yog coated nrog cov ntaub ntawv tshwj xeeb, uas khi rau DNA nyob rau qee yam. Thaum cov DNA yog khi, cov hlaws dai tau sib cais los ntawm cov qauv, ntxuav kom tshem tawm impurities, thiab tom qab ntawd DNA raug eluted. Tus txheej txheem no tsis yog ua kom nrawm dua cov kev pheej hmoo DNA tab sis kuj txo qis kev pheej hmoo ntawm tus qauv hla - kev txiav txim siab tseem ceeb hauv lub chaw kuaj. Tsis tas li ntawd, cov ntaub ntawv bluekit DNA ntau yam yog ntau yam, haum ntau yam qauv hom suav nrog cov kab piv txwv, ntaub so ntswg, thiab hlwb. Qhov kev hloov kho no ua rau nws muaj cov cuab yeej siv tsis thoob plaws thoob plaws kev tshawb fawb thiab kev kuaj mob, cov ntaub ntawv blueKit DNA ntau dua yog tsim nrog tus neeg siv hauv siab. Peb nkag siab tias lub sijhawm thiab yooj yim ntawm kev siv yog qhov tseem ceeb hauv kev ceev - kev tshawb fawb nyob ib puag ncig ntawm kev tshawb fawb. Yog li ntawd, peb cov khoom siv tau ua rau tus neeg siv - Tus phooj ywg, nrog nraim raws cov cai uas txuag tau lub sijhawm muaj txiaj ntsig yam tsis muaj kev txi. Seb koj puas tau tshawb fawb txog kev tshawb fawb siab dua lossis cov khoom siv DNA extures kom koj muaj kev nkag siab zoo, npaj rau cov kauj ruam tom ntej hauv koj qhov tseem ceeb. Tshawb nrhiav li cas cov blukit DNA extraction cov khoom siv tau tuaj yeem tsim lub peev xwm ntawm koj tus kws tshaj lij

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Cov lus qhia

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Cov khoom siv no yog tsim rau cov lus yooj yim thiab muaj txiaj ntsig zoo ntawm genome. Cov khoom siv no tuaj yeem thovTxhawm rau rho tawm cov khoom me me ntawm cov qauv thiab ua hauv qhov siab - Kev nthuav dav teevcia li.

Genomic DNA muab rho tawm los ntawm cov khoom siv no tuaj yeem siv tau los tshawb pom cov tswv yim ntawm tes ntawm tes DNA hauv qee qhov kev sim.