I-T7 RNA Ethuthukisiwe I-Mycoplasma DNA Ukutholwa Kit - Bluekit ZY002
I-T7 RNA Ethuthukisiwe I-Mycoplasma DNA Ukutholwa Kit - Bluekit ZY002
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Emhlabeni we-biology molefanelar, ukunemba nokwethenjwa ekutholeni ukungcola kubaluleke kakhulu. I-Bluekit's Mycoplasma DNA ukutholwa kwekhithi (QPCR) - I-ZY002 imele ngaphambili kwalesi sidingo esibalulekile, ifaka ubuchwepheshe obusha be-T7 RNA ukunikela ngokunemba okungenakuqhathaniswa nokushesha ekutholeni okune-Mycoplasma. Lokhu kusika - Ikhithi Ephephelwe yenzelwe ngqo abacwaningi abafuna amazinga aphezulu emsebenzini wabo, baqinisekise ukuthi amasampula abo akhululekile ekungcoleni kwami, okujwayelekile kodwa okuvamile kunoma iyiphi imvelo yelebhu.
Ukwakheka kwethu okuhlukile kufaka i-T7 RNA polymerase ukukhulisa inqubo yokutholwa kwe-DNA, okwenza kube nobucayi obukhulu kunezindlela zendabuko. Lokhu kuthuthukiswa kusho ukuthi ngisho nenani lemizuzu ye-Mycoplasma DNA ayinakwa, ukuqinisekisa ukuthembeka kwezivivinyo zakho kanye nemiphumela yocwaningo. Ilungele uhla olubanzi lwezinhlobo zesampula, i-Bluekit ZY002 ihlinzeka ngesisombululo esiguquguqukayo sendalo ebanzi yokungcola kwe-Mycoplasma, ukubhekisisa amasiko ahlukahlukene okusabela okubandakanya amaseli okusabela okungu-50, ngalinye ngokucophelela elenzelwe imiphumela ethembekile. Ukulula kwe-protocol, kuhlangene nokusika - Umphetho we-T7 RNA ubuchwepheshe, kunciphisa kakhulu isikhathi kusuka kusampula kuyaphumela, ukuvumela abacwaningi ukuba baqhubeke nokubambezeleka kwabo ngokuzethemba nangokubambezeleka okungadingekile. Ukufakwa kwe-T7 RNA akugcini nje ngokuthi yinqubo kuphela inqubo kodwa kuthuthukisa ukuzwela nokucaciswa kokutholwa kwe-Mycoplasma, ukukunikeza ithuluzi elinamandla ekuphishekeleni okuhle kwesayensi.
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Ukucacisa
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Ukuphendula okungu-50.
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Ijika elijwayelekile
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Ishidi le-data
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Ukwakheka kwethu okuhlukile kufaka i-T7 RNA polymerase ukukhulisa inqubo yokutholwa kwe-DNA, okwenza kube nobucayi obukhulu kunezindlela zendabuko. Lokhu kuthuthukiswa kusho ukuthi ngisho nenani lemizuzu ye-Mycoplasma DNA ayinakwa, ukuqinisekisa ukuthembeka kwezivivinyo zakho kanye nemiphumela yocwaningo. Ilungele uhla olubanzi lwezinhlobo zesampula, i-Bluekit ZY002 ihlinzeka ngesisombululo esiguquguqukayo sendalo ebanzi yokungcola kwe-Mycoplasma, ukubhekisisa amasiko ahlukahlukene okusabela okubandakanya amaseli okusabela okungu-50, ngalinye ngokucophelela elenzelwe imiphumela ethembekile. Ukulula kwe-protocol, kuhlangene nokusika - Umphetho we-T7 RNA ubuchwepheshe, kunciphisa kakhulu isikhathi kusuka kusampula kuyaphumela, ukuvumela abacwaningi ukuba baqhubeke nokubambezeleka kwabo ngokuzethemba nangokubambezeleka okungadingekile. Ukufakwa kwe-T7 RNA akugcini nje ngokuthi yinqubo kuphela inqubo kodwa kuthuthukisa ukuzwela nokucaciswa kokutholwa kwe-Mycoplasma, ukukunikeza ithuluzi elinamandla ekuphishekeleni okuhle kwesayensi.
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Ukucubungula
Amaphrothokholi
Ukucacisa
Ukuthumela nokubuyisa
Ukuqoshwa Kwevidiyo
Cat.no. Hg - zy002 $ 1,508.00
Ikhithi isetshenziselwa ukuthola kahle ubukhona bokungcola kwe-Mycoplasma kumabhange ama-Master Cell, ukusebenza kwama-cell cell, ama-virus sembewu, amaseli okulawula, namaseli wokwelashwa ngokwelashwa komtholampilo.
I-kit isebenzisa ubuchwepheshe be-qpcr - ubuchwepheshe be-fluorescent ukuqinisekisa ngokubhekiswa kwizidingo ze-MycoPlasma defencedRelated Direction ku-EP2.6.7 neJPXVII. Ingasemboza ama-Mycoplass angaphezu kwe-100 futhi ayinakusabela okuphambene nezinkinga ezihlobene kakhulu. Ukutholwa kushesha okungaqedwa kungakapheli amahora ama-2, ngokucaciswa okuqinile.
