I-Pichia Pastoris HCP (I-Post cell protein) kit desidation desida

Pichia pastoris HCP (Host Cell Protein) Residual Detection Kit

I-Pichia Pastoris HCP (I-Post cell protein) kit desidation desida

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[: 0 - Eyodwa - Isinyathelo Elisa Protocol - konga isikhathi (<2h isiyonke isikhathi sokufa)
[: 0 - I-Pichia Pastoris - Okucacisiwe - Akunasiphambano - Ukuvuselelwa nge-Cho / HCPS
[: 0 - Silungele - Ukusebenzisa - Akukho okuncishisiwe kwesampula okudingekayo

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Ukucubungula

Amaphrothokholi

Ukucacisa

Ukuthumela nokubuyisa

Ukuqoshwa Kwevidiyo

Ukusetshenziswa okuhlosiwe
Le kit yenzelwe ukutholwa kwenani lezinsalela ze-Host Cell Protein (HCP) emikhiqizweni ye-biopharmace asuselwa ku- I-Pichia Pastoris I-STRAING (E.G., GS115, X33), isebenzisa eyodwa - Isinyathelo se-Enzyme - I-Immunosorbent Assay (ELISA) Ukusetshenziswa kocwaningo kuphela. Hhayi ngezinjongo zokuxilonga.

Isimiso sokutholwa
Ikhithi isebenzisa a sandwich elisa Indlela:

Umgwengwezi: Imithombo microplate i-pre - ihlanganiswe nama-antibodies wezimvu ezimele I-Pichia Pastoris I-HCPS.
Ukufakwa: Amasampula / amaCalibrators angeziwe, alandelwa yi-HRP - I-Polyclonal Yezimvu Ezihlanganisiwe I-ANTI -I-Pichia Pastoris Ama-HCP antibodies.
Ukubona: Ngemuva kokugeza, i-TMB substrate ingezwe ukuthuthukiswa kombala. Ukusabela kuyema, futhi ukumunca kukalwa at I-450 nm.
Ukunqunyelwa: Ukuhlushwa kwe-HCP kubalwa kusuka ku-Curve ejwayelekile (Anclusion α HCP okuhlushwa).

Izici ezibalulekile

Akukho okuncishisiwe kwesampula okudingekayo (Kudingeka kuphela ukuqinisekiswa kwe-dilution).
Iphrothokholi eqondisiwe: Izinyathelo ezimbalwa, imiphumela esheshayo (
Ukucaciswa okuphezulu nokuthembela: Kwenzelwe I-Pichia Pastoris I-HCPS.

Amanothi wezobuchwepheshe

Amasampula ahambisanayo: Ama-superstants wesiko le-cell cell, ahlanzwa biologics.
Ibanga elinamandla: 1-100 ng / ml (ejwayelekile).
Ukuqondanisa okulawulayo: Ilungele ukuthuthukiswa kwenqubo kanye nokuhlolwa kwe-GMP Lot ukukhishwa.

Inqubo yokusebenzisa

1. Ukulingana
- Letha zonke izingxenye ekushiseni kwegumbi (≥20 min).
2. Isampula / I-Antibody Angeze
- Engeza amasampula / ama-calibrators: 100 μl / kahle
- Faka i-1x enzyme - I-antibogugated antibody: 100 μl / kahle (bheka inganekwane ①)
3. Ukufakwa
- Icwila ku-RT, ivikelwe ekukhanyeni, 600 RPM ngo-3 h (bheka inganekwane ③)
4. Ukugeza
- Geza 1: 300 μl / kahle 1x buffer (isikhundla mpo sepuleti)
- Geza 2 - 5: 300 μl / 1x buffer, ama-5 ukugeza okuphelele
- Pat owomile: Ngemuva kokugeza kokugcina, hlukana neplate ephepheni elimunca
I-5
- Faka i-TMB: 100 μl / kahle
- Thuthukisa: 10 imiz e-RT (ivikelwe ukukhanya)
6. Misa ukusabela nokufunda
- Faka Isixazululo Sokuma: 50 μl / kahle
- Misa: 5 imiz
- Funda: Od ngo-450 nm (ireferefere 620 NM)

Yini ebhokisini?

Ingxenye	Umkhiqizo Cha.	Ukucacisa	Ukufanisa
Pre - Caint anti - I-Pichia HCP microplate	I-PNA015	8 Wells × 12 imichilo	Pre - ehlanganiswe ne-polyclonal ye-polyclonal anti -I-Pichia Pastoris Ama-HCP antibodies. Kuvalwe ku-foil Peouches nge-desiccant. Isitolo esivikelwe ukukhanya.
I-Pichia HCP Calibrator	I-PNB015	2 vials	I-Lyophilesed Powder. Phindisela nge-500 μl calibrator diluent (PNC002). Vumela ama-5- 10 imizuzu yokuchithwa (isixazululo kufanele kube kucace). Isitolo esivikelwe ukukhanya.
Calibrator dilount	Pnc002	1.5 ml × 1 tube	Sula Isixazululo Sokuphindaphinda I-Pichia HCP Calibrator.
Assay dilount	Pne006	25 ml × 2 amabhodlela	Ukunciphisa amaCalibrators, amasampula kanye ne-enzyme - umphintu ohlanganisiwe. Qinisekisa into efanelekayo ye-dilution yamasampula amasha.
Geza Buffer (10 × gcwele)	Pnf001	25 ml × 2 amabhodlela	Ukugeza ngepuleti. Amakristalu angakha emazingeni okushisa aphansi - ancibilika ku-37 ° C ngaphambi kokusetshenziswa. Dlulisa i-10 × ngamanzi e-ultrapure.
I-HRP - I-Anti ye-Connjugated - I-Pichia HCP antibody (100 ×)	I-PNN009	I-120 μl × 1 tube	I-POLYClonal antilody. Dlulisa i-100 × nge-assay dilount ngaphambi kokusebenzisa. Isitolo esivikelwe ukukhanya.
I-TMB substrate	I-PND004	12 mL × 1 ibhodlela	Linganisa izinga lokushisa lasekamelweni (≥20 min) ngaphambi kokusetshenziswa. Isitolo esivikelwe ukukhanya.
Misa ikhambi	I-PNI002	6 ml × 1 ibhodlela	Iqukethe i-HCL. Gqoka ama-goggles futhi uvikele ukuxhumana kwesikhumba.
Amafilimu wokuvala uphawu	I-PNK001	3 amashidi	Ngokumboza imichilo emincane ye-microplate ukuvikela ukungcoliswa nokuhwamuka.

I-Bluekitbio izimisele ukuhlinzeka ngemikhiqizo engcono kakhulu embonini yocwaningo lokuhlola.

Imininingwane yokuthumela

Sinikeza ukuthuthwa okuqandisiwe kuyo yonke imiyalo. Imvamisa, i-oda lakho lizofika ngaphakathi kwe-5 zebhizinisi lebhizinisi e-United States nakwezinsuku eziyi-10 zebhizinisi lakwamanye amazwe. Kodwa-ke, sicela uqaphele ukuthi ukulethwa ezindaweni zasemakhaya kungathatha isikhathi eside.

Isikhathi Sokuhambisa: Ama-oda ajwayele ukucutshungulwa kungakapheli 1 - Izinsuku ezi-3 zebhizinisi. Lapho i-oda lakho selithunyelwe, uzothola i-imeyili yokuqinisekisa ngemininingwane yokulandela.

Imininingwane ebalulekile

Ukucutshungulwa kwe-oda: Ngemuva kokuthi i-oda selikhokhelwe, indawo yethu yokugcina izimpahla idinga isikhathi sokucubungula i-oda lakho. Uzothola isaziso uma i-oda lakho lithunyelwe.

Izikhathi zokulethwa: Ezimweni eziningi, iphakethe lizohanjiswa ngesikhathi esilinganiselwe sokufika. Kodwa-ke, usuku lwangempela lokulethwa lungathinteka amalungiselelo endiza, izimo zezulu, nezinye izinto zangaphandle. Isikhathi sokulethwa kwesikhathi sizoba isikhathi eside kunokujwayelekile kuma-oda afaka ama-preorder noma izinto ezenziwe ngezifiso. Sicela ubheke imininingwane yokulandelela ngosuku olunembile kakhulu lokulethwa.

Izinkinga zokuthumela: Uma uthola ukuthi iphakethe lakho alizange lihanjiswe ngesikhathi esibekiwe; Imininingwane yokulandelela ikhombisa ukuthi iphakethe lihanjisiwe kepha awuyitholanga; Noma iphakheji yakho ifaka izinto ezingekho noma ezingalungile noma ezinye izindaba ze-Logistics, sicela uxhumane nensizakalo yethu yamakhasimende kungakapheli izinsuku eziyi-7 ngosuku lokukhokha ukuze sikwazi ukubhekana nalezi zinkinga ngokushesha.

Ukulandela umkhondo

Lapho i-oda lakho lithunyelwe, uzothola i-imeyili enenombolo yokulandela umkhondo nesixhumanisi sokulandela ukuthunyelwa kwakho.

Ungalandela futhi i-oda lakho ngqo kwiwebhusayithi yethu ngokungena ngemvume ku-akhawunti yakho nokubuka umlando we-oda lakho.

Ukuthumela Imikhawulo

Sicela ugcwalise ikheli lomgwaqo ngokuningiliziwe, hhayi ibhokisi le-PO noma ikheli lezempi (APO). Ngaphandle kwalokho, bekuzodingeka sisebenzise i-EMS ukulethwa (kuhamba kancane kunabanye, kuthatha 1 - izinyanga ezimbili noma isikhathi eside).

Imisebenzi Yamasiko Nenqubomgomo Yentela

Uyacelwa ukuthi uqaphele ukuthi noma yimiphi imisebenzi yamasiko, izintela, noma imali yokungenisa etholwe ngesikhathi sokuthumela kungumsebenzi womthengi. Lawa macala ayahluka ngokuya ngezwe elikuyo futhi anqunywa yiziphathimandla zendawo.

Ngokuthenga kusuka kuwebhusayithi yethu, uyavuma ukukhokha noma yimiphi imisebenzi esebenzayo noma izintela ezihambisana ne-oda lakho. Asinacala ngokubambezeleka okubangelwa imvume yenkambiso.

Inqubomgomo ye-Pickup Package

Lapho i-oda lakho selifikile endaweni ekhethiwe ye-pickup noma indawo yokulethwa, sicela uqinisekise ukuqoqwa okusheshayo. Uma iphakethe lingathathwanga ngaphakathi kwesikhathi esibekiwe, sizothumela isikhumbuzi nge-imeyili noma nge-SMS. Kodwa-ke, uma iphakethe lingaqoqwanga esikhathini esibekiwe, futhi noma yikuphi ukulahleka noma ukulimala kwenzeka ngenxa yalokho, umthengi uzoba nesibopho. Sikukhumbuza ngomusa ukuthi uqoqe iphakethe lakho ngokushesha ukugwema noma yiziphi izingqinamba ezingaba khona.

Qaphela: Njengoba umkhiqizo wethu uwela ngaphansi kwesigaba esikhethekile, ukubuyiselwa kwemali kanye nokubuyiselwa kwemali akwamukelwa.