Nk ne-til cell ukunwebeka reagents (K562 Eleder cell)
Nk ne-til cell ukunwebeka reagents (K562 Eleder cell)
✔ I-Pre - i-IrAdadeated, i-Multi - I-Cytokine ithuthukisiwe ukwanda okuphezulu kwe-NK / TIL.
✔ Iqinisekiswe zombili izintambo zegazi nemithombo ye-PBMC - Imoto - Ekulungele.
✔ Pre - Ufanelekile Ukulethwa kwe-IND ngedatha ephelele.
Ukubuka konke:
Le khasi Nk / til ukwanda reagent liqukethe Amaseli wezobuciko e-K562 feeder Ukuveza il - 21 namanye ama-cytokines asemqoka. Lapho kuhlanganiswa nemidiya ejwayelekile yamaseli omzimba, lolu hlelo lunika amandla:
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Ukuphakama - ukunwetshwa kokusebenza kahle iwa- Amaseli we-nk nama-tils kusuka kuma-PBMCS noma nge-Cord Blood -
Phezulu - Abantu Abamsulwa (> 90% amaseli okuhlosiwe)
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Ukwenza kusebenze ngokushesha, kusebenze Ngaphansi kokusayina okuhle kwe-cytokine
Izicelo ezibalulekile
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Ukwenza kusebenze & ukwanda iwa- Amaseli we-nk, imoto - NKS, nama-tils
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Ukuthuthukiswa Kwenqubo: Ekuqaleni - ukwenziwa kwesiteji kwe-cell therapy flowsflows
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Ukukhiqiza i-GMP: Usekela Ind - enika amandla izifundo na- I-Post - Izinguquko zenqubo Yokuvunywa
Izinzuzo Zokulawula Nokusebenza
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I-GMP - Ukuthobela ukwakhiwa
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Kuguquguquki, ukuphakama okuphezulu - (kuya ku 1000 - Songa)
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Pre - iqinisekiswe ukusetshenziswa kwemitholampilo
Ishidi le-data
Ukusebenza kahle kweseli ye-NK kanye nokusebenza kwabanikeli abaningi
I-Donor - Ukuhluka kwe-Doon
Umphumela wokuthuthumela we-viral e - Hancc ekuguqukeni kweLentival kumaseli we-NK
I-NK Cell TransDuction: I-Viral E - HanCerc Outperforms Abancintisana eMoi 1 Iphaneli Yesobunxele: Flow Cytometry of Il - Amaseli we-15 nk
Iphaneli yesokudla: Flow Cytometry of Il - Amaseli angu-15 NK ngaphandle kwe-TransDuction Enhancer
I-Viral E - HanCanc vs. Abaqhudelana: t
Kwesokudla: Flow Cytometry of Car - T Amaseli ngaphandle kwe-TransDuction Enhancer
Umphumela wokuthuthumela we-viral e - HanCer a + i-viral e - hancer d inhlanganisela ekudluliseleni kwe-lentiviral kuma-HSC
Ukuqhathanisa Ukuqhathanisa kwe-HSC TransDuction Naiceliancy: I-Viral E - HanCera + Viral E - HanCerd inhlanganisela yokuqhathanisa izimbangi (moi = 5)
| Inombolo yomkhiqizo | Igama Lokudla Kwe-Reagentand | Umthamo (we- ~ 1 - 2e6 PBMC Ukuqala) |
| 1 | Serum - mahhala medium | 2 l |
| 2 | Il - 2 | Izinduku ezi-4 (200,000 IU / STECT) |
| 3 | I-0.9% ijova ye-nacl | ~ 500 ml |
| 4 | I-20% i-albhamuin yomuntu ye-serum | ~ 15 ml |
| 5 | Isixazululo se-FICOLL Lymphocyte Solication Solution | ~ 25 ml |
| 6 | I-NC - Igumbi elibala ama-200 (noma elilinganayo) | Njengoba kudingeka |
| 7 | 6 - Ipuleti kahle | 1 |
| 8 | I-T25 Flask | 1 |
| 9 | I-T75 Flask | 1 |
| 10 | I-T175 Flask | 2 |
| 11 | 640 cm² cell Culture Bag | 2 |
| 12 | I-15 ml centrifuge tube | 1 |
| 13 | 1.5 ml centrifuge tube | Njengoba kudingeka |
| 14 | 10 mL / 25 ml Pipettes | Njengoba kudingeka |
| 15 | 1 ml / 200 μl pipette amathiphu | Njengoba kudingeka |
Ukucacisa
| Ipharamitha yokusebenza | Ukucacisa |
| Nk / Imoto - I-NK Expnsion Fold | > 5,000 × (izinsuku eziyi-14) |
| Ukusebenza kweseli | ≥90% |
| Ukumsulwa kweseli (CD56⁺ / CD3⁻) | > 95% |
Imininingwane yokuthumela
Sinikeza ukuthuthwa okuqandisiwe kuyo yonke imiyalo. Imvamisa, i-oda lakho lizofika ngaphakathi kwe-5 zebhizinisi lebhizinisi e-United States nakwezinsuku eziyi-10 zebhizinisi lakwamanye amazwe. Kodwa-ke, sicela uqaphele ukuthi ukulethwa ezindaweni zasemakhaya kungathatha isikhathi eside.
Isikhathi Sokuhambisa: Ama-oda ajwayele ukucutshungulwa kungakapheli 1 - Izinsuku ezi-3 zebhizinisi. Lapho i-oda lakho selithunyelwe, uzothola i-imeyili yokuqinisekisa ngemininingwane yokulandela.
Imininingwane ebalulekile
Ukucutshungulwa kwe-oda: Ngemuva kokuthi i-oda selikhokhelwe, indawo yethu yokugcina izimpahla idinga isikhathi sokucubungula i-oda lakho. Uzothola isaziso uma i-oda lakho lithunyelwe.
Izikhathi zokulethwa: Ezimweni eziningi, iphakethe lizohanjiswa ngesikhathi esilinganiselwe sokufika. Kodwa-ke, usuku lwangempela lokulethwa lungathinteka amalungiselelo endiza, izimo zezulu, nezinye izinto zangaphandle. Isikhathi sokulethwa kwesikhathi sizoba isikhathi eside kunokujwayelekile kuma-oda afaka ama-preorder noma izinto ezenziwe ngezifiso. Sicela ubheke imininingwane yokulandelela ngosuku olunembile kakhulu lokulethwa.
Izinkinga zokuthumela: Uma uthola ukuthi iphakethe lakho alizange lihanjiswe ngesikhathi esibekiwe; Imininingwane yokulandelela ikhombisa ukuthi iphakethe lihanjisiwe kepha awuyitholanga; Noma iphakheji yakho ifaka izinto ezingekho noma ezingalungile noma ezinye izindaba ze-Logistics, sicela uxhumane nensizakalo yethu yamakhasimende kungakapheli izinsuku eziyi-7 ngosuku lokukhokha ukuze sikwazi ukubhekana nalezi zinkinga ngokushesha.
Ukulandela umkhondo
Lapho i-oda lakho lithunyelwe, uzothola i-imeyili enenombolo yokulandela umkhondo nesixhumanisi sokulandela ukuthunyelwa kwakho.
Ungalandela futhi i-oda lakho ngqo kwiwebhusayithi yethu ngokungena ngemvume ku-akhawunti yakho nokubuka umlando we-oda lakho.
Ukuthumela Imikhawulo
Sicela ugcwalise ikheli lomgwaqo ngokuningiliziwe, hhayi ibhokisi le-PO noma ikheli lezempi (APO). Ngaphandle kwalokho, bekuzodingeka sisebenzise i-EMS ukulethwa (kuhamba kancane kunabanye, kuthatha 1 - izinyanga ezimbili noma isikhathi eside).
Imisebenzi Yamasiko Nenqubomgomo Yentela
Uyacelwa ukuthi uqaphele ukuthi noma yimiphi imisebenzi yamasiko, izintela, noma imali yokungenisa etholwe ngesikhathi sokuthumela kungumsebenzi womthengi. Lawa macala ayahluka ngokuya ngezwe elikuyo futhi anqunywa yiziphathimandla zendawo.
Ngokuthenga kusuka kuwebhusayithi yethu, uyavuma ukukhokha noma yimiphi imisebenzi esebenzayo noma izintela ezihambisana ne-oda lakho. Asinacala ngokubambezeleka okubangelwa imvume yenkambiso.
Inqubomgomo ye-Pickup Package
Lapho i-oda lakho selifikile endaweni ekhethiwe ye-pickup noma indawo yokulethwa, sicela uqinisekise ukuqoqwa okusheshayo. Uma iphakethe lingathathwanga ngaphakathi kwesikhathi esibekiwe, sizothumela isikhumbuzi nge-imeyili noma nge-SMS. Kodwa-ke, uma iphakethe lingaqoqwanga esikhathini esibekiwe, futhi noma yikuphi ukulahleka noma ukulimala kwenzeka ngenxa yalokho, umthengi uzoba nesibopho. Sikukhumbuza ngomusa ukuthi uqoqe iphakethe lakho ngokushesha ukugwema noma yiziphi izingqinamba ezingaba khona.
Qaphela: Njengoba umkhiqizo wethu uwela ngaphansi kwesigaba esikhethekile, ukubuyiselwa kwemali kanye nokubuyiselwa kwemali akwamukelwa.
