Ikhithi ephezulu yokutholwa kwe-RCL I-Bluekit
Ikhithi ephezulu yokutholwa kwe-RCL I-Bluekit
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Endaweni yocwaningo lwezakhi zofuzo kanye nokuxilonga ama-molecular, ukunemba nokwethenjwa kubaluleke kakhulu. I-Bluekit ngokuziqhenya ingenisa i-RCL (VSVG) yohlobo lwenombolo yohlobo lokutholwa kwenombolo (QPCR), umbuso - we - The Art Solution eyenzelwe ososayensi nabaphenyi abafuna amazinga aphezulu okunemba nokusebenza kahle emsebenzini wabo. Lo mkhiqizo omusha umi njengoTestament ekuzibophezele kwe-BlueKit ukuthuthukisa ukutholakala kwesayensi ngokuthuthukiswa kokusika - amathuluzi onqenqemeni.
Ikhithi yokutholwa kwe-RCL ithuthukiswe ngokucophelela ukuhambisa inqubo yokunciphisa inani lekhophi le-RCL gene, lifaka ubuchwepheshe kanye nokunemba kobuchwepheshe be-PCR (QPCR). Leli khithi alikho lula kuphela inqubo eyinkimbinkimbi yokuhlaziywa kwezakhi zofuzo kodwa futhi ihlinzeka abasebenzisi ngendlela ethembekile yokuthola imiphumela ephelele futhi enembile. Noma ngabe wenza ucwaningo oluyisisekelo, wenza ukuxilonga kwemitholampilo, noma ubandakanyeke ekuthuthukisweni kwemikhiqizo yokwelapha, le kit yakhelwe ukufeza izidingo zakho ngokusebenza kahle kwe-KIT design yayo yomsebenzisi wayo - I-Centric indlela. Ukuqonda izinselelo ezibhekene nabacwaningi ekuhlaziyweni kwenombolo zofuzo, sifake izici ezithuthukise ukusebenziseka futhi ziqinisekise ukuthembeka kwemiphumela. I-KIT ifaka i-datasheet ephelele ne-Curve Protocols evamile, abasebenzisi abaqondisayo ngesinyathelo ngasinye ngokucaca nokunemba. Ngaphezu kwalokho, ama-reacegents alungiselelwe kahle kanye ne-protocol ekhethiwe ngokucophelela enciphisa amandla amaphutha, aqinisekise ukuthi ngisho nalawa okusha ku-QPCR angafinyelela uchwepheshe - imiphumela yezinga. Ngekhithi yokutholwa kwe-RCL, ama-Bluekit anika amandla abacwaningi ukucindezela imingcele yokuhlaziya izakhi zofuzo, ukuvula iminyango ekutholweni okusha kanye nezinto ezintsha emkhakheni wezakhi zofuzo zamangqamuzana.
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Ijika elijwayelekile
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Ishidi le-data
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Ikhithi yokutholwa kwe-RCL ithuthukiswe ngokucophelela ukuhambisa inqubo yokunciphisa inani lekhophi le-RCL gene, lifaka ubuchwepheshe kanye nokunemba kobuchwepheshe be-PCR (QPCR). Leli khithi alikho lula kuphela inqubo eyinkimbinkimbi yokuhlaziywa kwezakhi zofuzo kodwa futhi ihlinzeka abasebenzisi ngendlela ethembekile yokuthola imiphumela ephelele futhi enembile. Noma ngabe wenza ucwaningo oluyisisekelo, wenza ukuxilonga kwemitholampilo, noma ubandakanyeke ekuthuthukisweni kwemikhiqizo yokwelapha, le kit yakhelwe ukufeza izidingo zakho ngokusebenza kahle kwe-KIT design yayo yomsebenzisi wayo - I-Centric indlela. Ukuqonda izinselelo ezibhekene nabacwaningi ekuhlaziyweni kwenombolo zofuzo, sifake izici ezithuthukise ukusebenziseka futhi ziqinisekise ukuthembeka kwemiphumela. I-KIT ifaka i-datasheet ephelele ne-Curve Protocols evamile, abasebenzisi abaqondisayo ngesinyathelo ngasinye ngokucaca nokunemba. Ngaphezu kwalokho, ama-reacegents alungiselelwe kahle kanye ne-protocol ekhethiwe ngokucophelela enciphisa amandla amaphutha, aqinisekise ukuthi ngisho nalawa okusha ku-QPCR angafinyelela uchwepheshe - imiphumela yezinga. Ngekhithi yokutholwa kwe-RCL, ama-Bluekit anika amandla abacwaningi ukucindezela imingcele yokuhlaziya izakhi zofuzo, ukuvula iminyango ekutholweni okusha kanye nezinto ezintsha emkhakheni wezakhi zofuzo zamangqamuzana.
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Inombolo
Ukucubungula
Amaphrothokholi
Ukucacisa
Ukuthumela nokubuyisa
Ukuqoshwa Kwevidiyo
Cat.no. Hg - rc001 $ 1,508.00
Le kit yenzelwe ukutholwa kwenani le-RCL gene Copy Copy in the genome of Car - tAmaseli alungiselelwe ngokusebenzisa i-HIV - 1 ubuchwepheshe be-vector le-lentiviral.
Le kit yamukela indlela ye-fluorescent phone kanye nendlela ye-multex PCR yokuthola ukulandelana kwe-DNAokuhlobene nokuhlanganiswa noma umsebenzi wokubonisa kwi-plasmid yokudlulisa, kanye nenombolo ye-vvg gene CopyKusampula kungabalwa. I-KIT iyithuluzi elisheshayo, eliqondile nelithembekile.
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Ibanga le-Assay |
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Umkhawulo we-maciation |
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Umkhawulo wokutholwa |
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Ukwenza kahle |
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