I-Cell Cytotoxicity Assay Kit (amaseli ahlanganisiwe asamisiwe)
I-Cell Cytotoxicity Assay Kit (amaseli ahlanganisiwe asamisiwe)
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✔Dual - ilebula cytotoxicity detection (CFSE + 7 - AAD)
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✔Imishini yokumiswa kwemigqa ye-tumor cel cell
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I-I✔ Vala i-Car - T / NK Cell Therapies
Ukubuka konke:
Le khasi Ukumiswa kwelitshe le-cytotoxicicity assay kit yakhelwe ukukala iseli eflensi - i-cytotoxicity elungisiwe ngokumelene ukumiswa - amaseli ahlosiwe amasiko. Ikhithi iqukethe:
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CKE (I-CarboxyFluensiin SuccucnidIdIdyl Ester): Udayi we-streand
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7 - Isadi (7 - I-Aminoactomycin d): Isixhumanisi se-DNA sokulebula / amaseli alonakalisiwe
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Isiko Medium a -AngathiI-Assay Buffer
Izinja
Ukuhlola okusebenzayo kwamaseli we-immune aunter (Amaseli e-NK, Imoto - NK, Imoto - T, TCR - T, amaseli we-TM) Ukuphikisana nokumiswa kwama-stumel cell
✔ Ukuthuthukiswa Kwenqubo: Lungiselela ukugeleza kokwelashwa kwamaselula
✔ Ikhwalithi yokulawula: Ukukhishwa kwezifundo zokuhlola kanye nokuqina kwemitholampilo - Imikhiqizo yesigaba
Izici ezibalulekile
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Dual - Uhlelo Lokulebula: CFSE (okuhlosiwe okubukhoma) + 7 - I-AAD (amaseli afile) ngokuqanjwa kwe-cytotoxicity okuqondile
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I-GMP - Iyasebenza: Kuqinisekiswe ukuthuthukiswa kokwelashwa kwamaselula ngaphansi kwemihlahlandlela ye-FDA / EMA
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Ukugeleza komsebenzi - usukulungele: Pre - Kwenzelwe amaphrothokholi wokuhlanganiswa okungenamthungo
Amaphuzu Avelele Ezobuchwepheshe
▸ Ukuguquguquka kwe-cell cell: Iyahambisana nemigqa ye-tumor evamile (e.g., K562, Raji)
▸ Ukuqondanisa okulawulayo: Usekela i-IT - enika amandla izifundo ezinamaphakeji wedokhumenti
▸ Ukuvezazela: ≤10% CV nge-Inter - Ukuhlolwa kwe-Assay ngokunemba
Ishidi le-data:
Ukuhlaziywa kwe-cytometry ye-GLOME ye-NK cell - I-Cytotoxicicity elungisiwe ngokumelene ne-CFSE - Ilebula elikhonjisiwe k562 amaseli okuhlosiwe
I-NK cell cytotoxicity ukubulala ijika ngokumelene ne-CFSE - Ilebula elibhalwe phansi k562 amaseli okuhlosiwe
| I-GATALOCATION ID | Ingxenye | Ubuningi | Izimo Zokugcina | Indawo |
|---|---|---|---|---|
| Hg - md001 | Medium a | I-100 ml | 2 - 8 ° C | Kit i |
| Hg - fw001 | I-Assay Buffer | I-100 ml | 2 - 8 ° C | - |
| Hg - cfse | I-CFSE Stain | 1 vial | - 20 ° C | Kit II |
| Hg - 7 - Isadi | 7 - | 1 vial | - 20 ° C | - |
Ukucaciswa
| Ipharamitha yokusebenza | Ukwemukelwa |
| Ukuqonda nqo | Ukuthola kabusha 70 - 130% |
| Ukuphambuka | I-Bias 70 - 130% |
| -Phindaphiwe | I-CV ≤ 10% |
| Ukunemba okuphakathi | I-CV ≤ 10% |
Imininingwane yokuthumela
Sinikeza ukuthuthwa okuqandisiwe kuyo yonke imiyalo. Imvamisa, i-oda lakho lizofika ngaphakathi kwe-5 zebhizinisi lebhizinisi e-United States nakwezinsuku eziyi-10 zebhizinisi lakwamanye amazwe. Kodwa-ke, sicela uqaphele ukuthi ukulethwa ezindaweni zasemakhaya kungathatha isikhathi eside.
Isikhathi Sokuhambisa: Ama-oda ajwayele ukucutshungulwa kungakapheli 1 - Izinsuku ezi-3 zebhizinisi. Lapho i-oda lakho selithunyelwe, uzothola i-imeyili yokuqinisekisa ngemininingwane yokulandela.
Imininingwane ebalulekile
Ukucutshungulwa kwe-oda: Ngemuva kokuthi i-oda selikhokhelwe, indawo yethu yokugcina izimpahla idinga isikhathi sokucubungula i-oda lakho. Uzothola isaziso uma i-oda lakho lithunyelwe.
Izikhathi zokulethwa: Ezimweni eziningi, iphakethe lizohanjiswa ngesikhathi esilinganiselwe sokufika. Kodwa-ke, usuku lwangempela lokulethwa lungathinteka amalungiselelo endiza, izimo zezulu, nezinye izinto zangaphandle. Isikhathi sokulethwa kwesikhathi sizoba isikhathi eside kunokujwayelekile kuma-oda afaka ama-preorder noma izinto ezenziwe ngezifiso. Sicela ubheke imininingwane yokulandelela ngosuku olunembile kakhulu lokulethwa.
Izinkinga zokuthumela: Uma uthola ukuthi iphakethe lakho alizange lihanjiswe ngesikhathi esibekiwe; Imininingwane yokulandelela ikhombisa ukuthi iphakethe lihanjisiwe kepha awuyitholanga; Noma iphakheji yakho ifaka izinto ezingekho noma ezingalungile noma ezinye izindaba ze-Logistics, sicela uxhumane nensizakalo yethu yamakhasimende kungakapheli izinsuku eziyi-7 ngosuku lokukhokha ukuze sikwazi ukubhekana nalezi zinkinga ngokushesha.
Ukulandela umkhondo
Lapho i-oda lakho lithunyelwe, uzothola i-imeyili enenombolo yokulandela umkhondo nesixhumanisi sokulandela ukuthunyelwa kwakho.
Ungalandela futhi i-oda lakho ngqo kwiwebhusayithi yethu ngokungena ngemvume ku-akhawunti yakho nokubuka umlando we-oda lakho.
Ukuthumela Imikhawulo
Sicela ugcwalise ikheli lomgwaqo ngokuningiliziwe, hhayi ibhokisi le-PO noma ikheli lezempi (APO). Ngaphandle kwalokho, bekuzodingeka sisebenzise i-EMS ukulethwa (kuhamba kancane kunabanye, kuthatha 1 - izinyanga ezimbili noma isikhathi eside).
Imisebenzi Yamasiko Nenqubomgomo Yentela
Uyacelwa ukuthi uqaphele ukuthi noma yimiphi imisebenzi yamasiko, izintela, noma imali yokungenisa etholwe ngesikhathi sokuthumela kungumsebenzi womthengi. Lawa macala ayahluka ngokuya ngezwe elikuyo futhi anqunywa yiziphathimandla zendawo.
Ngokuthenga kusuka kuwebhusayithi yethu, uyavuma ukukhokha noma yimiphi imisebenzi esebenzayo noma izintela ezihambisana ne-oda lakho. Asinacala ngokubambezeleka okubangelwa imvume yenkambiso.
Inqubomgomo ye-Pickup Package
Lapho i-oda lakho selifikile endaweni ekhethiwe ye-pickup noma indawo yokulethwa, sicela uqinisekise ukuqoqwa okusheshayo. Uma iphakethe lingathathwanga ngaphakathi kwesikhathi esibekiwe, sizothumela isikhumbuzi nge-imeyili noma nge-SMS. Kodwa-ke, uma iphakethe lingaqoqwanga esikhathini esibekiwe, futhi noma yikuphi ukulahleka noma ukulimala kwenzeka ngenxa yalokho, umthengi uzoba nesibopho. Sikukhumbuza ngomusa ukuthi uqoqe iphakethe lakho ngokushesha ukugwema noma yiziphi izingqinamba ezingaba khona.
Qaphela: Njengoba umkhiqizo wethu uwela ngaphansi kwesigaba esikhethekile, ukubuyiselwa kwemali kanye nokubuyiselwa kwemali akwamukelwa.
