Ikhithi yokutholwa enembile ye-VSVG - I-RCL gene
Ikhithi yokutholwa enembile ye-VSVG - I-RCL gene
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Esikhathini se-realm ye-biology ye-molecular kanye nocwaningo lwezakhi zofuzo, ukunemba nokwethenjwa kokuthola izinombolo ezithile zohlobo lwezakhi zofuzo kubaluleke kakhulu. Ukusika kwe-Bluekit - I-Edge VSVG Detection Kit, eyenzelwe ukwakhiwa kwe-Retroviral Coreceplor Ligand (RCL) gene Copy Copy nge-QPCR indlela, imikhakha yokwenziwa okusha kulo mkhakha. Le khithi akuyona nje umkhiqizo; Yithuluzi elijabulisayo labacwaningi nososayensi okuhlose ukucindezela imingcele yokuqonda ngozakhi zofuzo kanye nezicelo.
Izisekelo zekhithi yethu yokutholwa kwe-VSVG zakhiwe phezu kohlaka olunzima lwesayensi, ukuqinisekisa ukuthi ingxenye ngayinye inomthelela ekunembeni nasekusebenzeni kahle kwenqubo ye-QPCR. I-kit iza iphelele ngomumo - ukusebenzisa ama-reagents, ijika elijwayelekile eliklanywe ngokucophelela ngokulingana, kanye nedatha ephelele. Ngayinye yalezi zinto ihambisana nokuluntsha ukuhamba komsebenzi, ukunciphisa amaphutha angenzeka, futhi ulethe imiphumela ephindeka. Ngokugxila ezintweni ezisemqoka ze-QPCR Mediated RCL gel Gene Copy Dection Inombolo, i-Bluekit inikeza isixazululo sobabili umsebenzisi - enobubanzi bezidingo zocwaningo. Ijika elijwayelekile, ingxenye ebucayi yekhithi, itholakala ngezifanekiso ezibonakala ngokucophelela, iqinisekise ukuthi abasebenzisi bakwazi ukunciphisa izinombolo ze-RCL gelco copy kumasampula abo ngokuzethemba. Lokhu, kuhlangene ne-reacents ye-kit eyenziwe kahle, iqondisa inqubo ye-QPCc, okwenza itholakale kalula ezindaweni zokusebenzela amakhono ahlukahlukene. Noma ngabe kungocwaningo, ukuxilonga, noma ukuthuthukiswa kwezokwelapha, i-vsvg ukuthola ukutholwa kwe-vsvg injiniyela ukwenza lula imizamo yakho yesayensi, ukuvumela ukuphumelela kanye nokuthuthuka ekuqondeni imisebenzi yofuzo nemiphumela yazo.
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Ijika elijwayelekile
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Ishidi le-data
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Izisekelo zekhithi yethu yokutholwa kwe-VSVG zakhiwe phezu kohlaka olunzima lwesayensi, ukuqinisekisa ukuthi ingxenye ngayinye inomthelela ekunembeni nasekusebenzeni kahle kwenqubo ye-QPCR. I-kit iza iphelele ngomumo - ukusebenzisa ama-reagents, ijika elijwayelekile eliklanywe ngokucophelela ngokulingana, kanye nedatha ephelele. Ngayinye yalezi zinto ihambisana nokuluntsha ukuhamba komsebenzi, ukunciphisa amaphutha angenzeka, futhi ulethe imiphumela ephindeka. Ngokugxila ezintweni ezisemqoka ze-QPCR Mediated RCL gel Gene Copy Dection Inombolo, i-Bluekit inikeza isixazululo sobabili umsebenzisi - enobubanzi bezidingo zocwaningo. Ijika elijwayelekile, ingxenye ebucayi yekhithi, itholakala ngezifanekiso ezibonakala ngokucophelela, iqinisekise ukuthi abasebenzisi bakwazi ukunciphisa izinombolo ze-RCL gelco copy kumasampula abo ngokuzethemba. Lokhu, kuhlangene ne-reacents ye-kit eyenziwe kahle, iqondisa inqubo ye-QPCc, okwenza itholakale kalula ezindaweni zokusebenzela amakhono ahlukahlukene. Noma ngabe kungocwaningo, ukuxilonga, noma ukuthuthukiswa kwezokwelapha, i-vsvg ukuthola ukutholwa kwe-vsvg injiniyela ukwenza lula imizamo yakho yesayensi, ukuvumela ukuphumelela kanye nokuthuthuka ekuqondeni imisebenzi yofuzo nemiphumela yazo.
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Inombolo
Ukucubungula
Amaphrothokholi
Ukucacisa
Ukuthumela nokubuyisa
Ukuqoshwa Kwevidiyo
Cat.no. Hg - rc001 $ 1,508.00
Le kit yenzelwe ukutholwa kwenani le-RCL gene Copy Copy in the genome of Car - tAmaseli alungiselelwe ngokusebenzisa i-HIV - 1 ubuchwepheshe be-vector le-lentiviral.
Le kit yamukela indlela ye-fluorescent phone kanye nendlela ye-multex PCR yokuthola ukulandelana kwe-DNAokuhlobene nokuhlanganiswa noma umsebenzi wokubonisa kwi-plasmid yokudlulisa, kanye nenombolo ye-vvg gene CopyKusampula kungabalwa. I-KIT iyithuluzi elisheshayo, eliqondile nelithembekile.
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Ibanga le-Assay |
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Umkhawulo we-maciation |
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Umkhawulo wokutholwa |
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Ukwenza kahle |
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