Uyini umehluko phakathi kweTrypsin nezinye izithonjana?

Isingeniso sokuvikela ezinhlelweni zebhayoloji

Ama-proterises, iqembu elihlukahlukene lama-enzyme, anobuhlakani ezinqubweni eziningi zebhayoloji, kusuka ekugayweni kuya kwisibonakaliso seseli. Basebenza nge-Catalyzing the Cleavage of Peptide Bonds kumaprotheni, ngaleyo ndlela badlala indima ebalulekile ekugcineni i-Homeostasis yamaselula. Ama-proteries ahlukaniswa ngokususelwa kumakhethini awo we-catalytic kanye nokucaciswa kwe-substrate, ngamaphrofetha e-serine afana ne-trypsin efundwa kakhulu. Lo mbhalo uhlola izimfanelo ezihlukile ze-trypsin ngokungafani namanye ama-proterises, cabanga ngezinto ezifana nesakhiwo, ukusebenza kanye nezicelo.

I-Trypsin: Izinga legolide kuma-proteomics

Indima ye-trypsin ku-Mass Spectrometry

I-Trypsin iholela enkampanini yama-proteomics ngenxa yokucaciswa kwayo okuphezulu kanye nokusebenza kahle ekubambeni amaketanga we-peppide e-carboxyl ohlangothini lwe-lysine nezinsalela ze-arginine. Lokhu kucaciswa kuphumela kwezingcezu ezinecala elifanele ku-C labo Kuma-ama-protemics, amandla ka-trypsin ukukhiqiza ama-peptides wobude obulungile bakhulisa ukukhonjwa kwamaprotheni, isici esibalulekile socwaningo lwe-cell therapy, lapho kunesimo esinembile samaphrotheni.

Izinzuzo ekuhlonzeni kwamaprotheni

Amaprotheni athembele kakhulu ezingxenyeni ezihlukile zeTrypsin zokuhlonza amaprotheni. Ucwaningo lukhombisa ukuthi i-trypsin ingakhomba amaprotheni angaphezulu kwama-80% aqhathaniswa nokuvikela ngokucaciswa okubanzi, okwenza kubaluleke kakhulu kubacwaningi nabaphakeli ensimini. Indima yayo ifinyelela ekuqondeni izindlela zokuqonda izindlela zokwelapha, njengokulashwa kwamaseli.

Izici zesakhiwo zeTrypsin

Isayithi elisebenzayo kanye nendlela e-catalytic

Ukusebenza kahle kwe-catalytic kwe-trypsin kusuka emthonjeni wayo - kuchazwe indawo esebenzayo, enezimpawu ze-catalytic ze-serine, i-histidine, kanye ne-amplartate. Lokhu kucushwa kusiza ukuqinisekiswa kwebhondi ye-peptide bond cleavage, isidingo esibalulekile sabahlinzeki abahlinzeka ngama-enzyme ahlanziwe ocwaningweni kanye nezicelo zezimboni. Ukucaciswa kwe-enzyme kubangelwa ubukhona bezinsalela zezinsalela ezikhokhiswe kabi, eziheha ukukhokhiswa kahle kwe-Lysine ne-Argine.

Ama-isoforms we-trypsin

I-Trypsin ikhona kuma-isoforms amaningana, kufaka phakathi i-cationic, anionic, ne-mesotrypsinogen. I-isoform ngayinye isebenza izindima ezihlukile, nge-cotionic trypsin ibe yiyona ende kakhulu kumanyikwe komuntu. I-Mesotryplin iyaphawuleka ngokumelana kwayo ku-inhibitors, ukudlala indima ekhethekile ekucekelweni kwe-inhibitor - amaprotheni okudla acebile. Ukuqonda lezi zesoform zibalulekile ukuze amafektri ahilelekile ekukhiqizweni kwe-enzyme ukuze asetshenziswe ngokwelashwa.

Amaphrothenisi ahlukile kanye nokucaciswa kwawo

Qhathanisa ne-aspn ne-gluc

Izinsalela ze-ASPN ne-Gluc ezihlosiwe ze-acidic acidic Amino, zinikeza idatha ehambisanayo kulokho okunikezwe yi-Trypsin. Lawa ma-proteries akhiqiza izingxube eziyinkimbinkimbi ze-peptide ezilungele izidingo ezithile zokuhlaziya kuma-proteromics. Kodwa-ke, ukucaciswa kwabo okubanzi kuvame ukuholela ekuhlolweni okuncishisiwe kwamaprotheni kuqhathaniswa ne-trypsin.

I-Chymotryplin kanye nokucaciswa okubanzi

I-Chymotryplin yehluka kusuka ku-trypsin ngokuqondisa izinsalela ezinkulu ze-hydrophobic, njenge-tryptophan ne-tyrosine. Ngenkathi iphatha ukusebenza kahle okukhulu kwe-catalytic, ukusetshenziswa kwayo kuvame ukukhawulelwa kwizicelo ezithile. I-Bround - Proefity Proteases efana neProteinase K yakhiqiza izingxube eziyinkimbinkimbi kakhulu, zethula izinselelo zezicelo zama-proteimics, ngaphandle kokuthi kusetshenziswe ngokuhlanganiswa ne-trypsin.

Amasu okugaya alandelanayo nge-trypsin

Ukukhonjwa kwamaprotheni okuthuthukisiwe

Ukugaya okulandelanayo, okubandakanya ukusetshenziswa kwe-trypsin kulandelwa enye proteise, kuthuthukisa kakhulu ukukhonjwa kwamaprotheni. Isibonelo, ukubikezela nge-Trypsin kungakhuphula ukuhlonza amaprotheni we-proteinase K ngo-731%. Okwezicelo zokwelashwa kwamaselula, lo msebenzi ovumelanayo uvumela amaprotheni aphelele ama-proteomic afaka amaprotheni, acucu ngokwengeziwe wokuqonda izindlela zamaselula.

Ukuvikelwa kwama-peptides ngokulandelana kokuhlelwa okulandelanayo

Ama-peptides amancane akhiqizwe yi-trypsin avikelekile ekugayweni okwengeziwe ezinqubweni ezilandelanayo, okuholela ekubumbeni okuncane kunokubikezelwa ekuhlaziyweni kwe-silico. Le ndlela inenzuzo kubahlinzeki abahlinzeka ngezinsizakalo zamaprotheni, njengoba iqinisekisa ukunemba okuphezulu ekuhlonzeni kwamaprotheni kanye nokuhlongona.

Ukucaciswa kokuqhathanisa ukucacisa okubanzi

Proteres ngokucaciswa okuphezulu, njengeTrypsin, athandwa ngenxa yekhono lawo lokukhiqiza izingcezwana ezibonakalayo nezilawuleke. Ngokuphambene nalokho, ukucaciswa okubanzi kuholela ekuxubeni okuyinkimbinkimbi, okuxakayo kwedatha. Amafektri akhiqiza ama-enzyme ocwaningweni kufanele acubungule lo mehluko ukuze ahlangabezane nezidingo zemisebenzi ethile yamaprotheni ahlukahlukene.

Indima yama-zymogens ekusebenzeni kwe-protache

Protate eresersor activation

Ama-proteries avame ukuhlanganiswa njengama-zymogen angasebenzi ukuvikela ukubhujiswa okungafuneki kwamaprotheni amaselula. I-Trypsinogen, eyenziwe yasebenza emathunjini amancane, ibonisa lo mqondo kahle. Ukuqonda kusebenze i-zymogen kubalulekile ukuthi amafektri akhiqiza amaphrofeyithi ukusetshenziswa kokwelapha, ukuqinisekisa ukuthi ama-enzyme asebenzayo ahlinzekwa ngezimo ezilawulwayo.

Umthethonqubo ngu-Entokinase

I-EnterKinase idlala indima ebalulekile ekusebenzeni kwe-trypsinogen, igcizelela ukubaluleka kokulawulwa okuqondile ekusebenzeni kwe-proteaseache. Ibhalansi phakathi kwe-zymogen synthesis kanye nokwenza kusebenze kuqinisekisa ukuthi umsebenzi weProteolytic kwenzeka kahle ngaphakathi komzimba, okungcono kakhulu kwabahlinzeki abahlinzeka ngezinhlelo zokulethwa kwe-enzyme.

Ama-protute inhibitors kanye nemisebenzi yabo yemvelo

Ukulawulwa komsebenzi weprotheni

Ama-protease inhibitors abalulekile ekulandiseni umsebenzi weprotheni ngaphakathi kwezinhlelo zebhayoloji. Bavimba ukonakaliswa okungalawulwa kwamaprotheni, ukulinganisela okubalulekile ezintweni eziphakeme. Ekwelashweni kwamaseli, ukuqonda lokhu kubhala kubalulekile ekwakheni ukwelashwa okuhlanza umsebenzi wokuvikela ukusebenza ngempumelelo.

Ama-protutease inhibitors kuma-Therapeutiums

Ama-protease inhibitors awagcini nje ngokuba nezindima kuphela emthethweni we-physiologioliologiolioliog kodwa futhi nasezisetshenzisweni zokwelapha. Banikela ngezinzuzo ezingaba khona, njengokuvimbela ukulimala kwezinso kusuka kuma-antibiotic noma ekwehliseni ubungozi bezitho zabala, okukhombisa ukubaluleka kwabo ocwaningweni lwezokwelapha kanye nentuthuko.

Izicelo zezimboni kanye ne-biotechnological of trypsin

Indima eBiotechnology

I-Trypsin isetshenziswa kabanzi ezinqubweni ze-biotechnological, njengokugaya amaprotheni kanye nokuhlukaniswa kwamaseli. Ukucaciswa kwayo nokusebenza kwayo kwenza kube yinto ekhethwayo yezicelo eziningi, kufaka phakathi amafektri akhiqiza imikhiqizo yokwelashwa kwamaseli, lapho ukucaciswa komsebenzi we-enzymatic kubalulekile.

I-Bluekit ihlinzeka ngezixazululo

I-Bluekit inikeza izixazululo ezibanzi ezihambelana nokuhlangabezana nezidingo ze-protease zocwaningo kanye nezicelo zezimboni. Ngokuhlinzeka okuphezulu - I-Trypsin yekhwalithi nokunye okuvikelayo, i-BlueKit iqinisekisa ukuthi ama-laboratories kanye namafektri anamathuluzi adingekayo ukuze athuthukise umsebenzi wawo kuma-proteomics, iselula yezokwelapha, kanye ne-biotechnology. Ukuzibophezela kwethu kwikhwalithi kanye nezinto ezintsha kusibeka njengomhlinzeki oholayo, kukusiza ukuba uthole imiphumela ethembekile futhi eqondile emikhakheni yakho yesayensi.