ISINGENISO KWESIMANGALISO SAMAHHALA IL - 2 Kits Detection Kits
● Ukuchazwa kanye nokubaluleka
I-Interleukin - 2 (Il - 2) iyindlela ePivotal Cytokine kumasosha omzimba womuntu, ngokuyinhloko ebandakanyeka ekuqondisweni kwamangqamuzana amhlophe egazi, okubalulekile ekuphenduleni komzimba komzimba. Ama-kits e-Human Il - 2 Amakhithi okutholwa angamathuluzi asemqoka asetshenziswa kuzo zombili izilungiselelo zocwaningo nezilungiselelo zemitholampilo ukukala okuhlushwa kwe-IL - 2 kumasampula ahlukahlukene okuzalwa. Lawa ma-kits asemqoka ukuthuthukisa ukuqonda kwethu izimpendulo zomzimba kanye nokuthuthukiswa kokwelashwa okusha, ukuthuthukisa ukusebenza kwezicelo zokwelashwa kwamaselula.
Ukubaluleka kwe-IL - 2 Ukutholwa kufinyelela emikhakheni eminingana, kufaka phakathi i-immunology kanye ne-oncology, lapho ukuqonda izimpendulo zokuzivikela komzimba kungaholela ekutheni izimpendulo zokuzivikela komzimba kungaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo ze-Whish zingaholela ekutheni izimpendulo ze-Whim -fion zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zokuzivikela komzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela ekutheni izimpendulo zomzimba zingaholela entuthukweni yokuhlaselwa komhlaba ekwelapheni. Njengoba imboni ye-biotech ivela, isidingo somuntu oqondile, onokwethenjelwa, futhi osebenza kahle
● Izinhlobo zamasampula aphilayo asetshenzisiwe
IlOnian Il - 2 kit detectionAma-S angasetshenziswa kumasampula ahlukahlukene okuzalwa, kufaka phakathi i-serum, i-plasma, ama-superstantants esiko leseli, negazi eliphelele. Uhlobo ngalunye lwesampula luhlinzeka ngokuqonda nezinselelo ezihlukile, okwenza ukuguquguquka kwekhithi lokutholwa kube yinto ebalulekile ekusebenzeni kwayo. Isibonelo, ukuhlaziya i-Cell Culture Speastants Super Naturants kunganikeza imininingwane eningiliziwe ekukhiqizeni i-cytokine eVitro, okubaluleke kakhulu ezifundweni zemvelo zama-mic-amkhorapapies amasha ambalwa.
Indlela ye-Il - Ukutholwa 2
● Indima yama-antibodies nama-enzyme
Umnyombo we-Mil Il - 2 Ama-Kits atholwayo athembele ekusebenziseni ama-antibodies nama-enzyme athola ngokukhethekile futhi akwazi ukukala il - ama-molecule ama-2. Imvamisa, lezi zikhithi zisebenzisa iSandwich ELISA (Enzyme - Ixhumeke I-Immunosorbent Assay) Formay. Kule ndlela, il - 2 Amanje kusampula athunjwa phakathi kwezendlalelo ezimbili zama-antibodies: ukuthwebula i-antibody eboshwe endaweni eqinile kanye ne-antibod eqinile exhumene ne-enzyme. I-enzyme iphendula nge-substrate ukukhiqiza ushintsho lwe-colorimetric olungalinganiswa lungabonakali olubonakalayo.
Lokhu kuzwela kanye nokucaciswa kwendlela kuncike kakhulu kwikhwalithi yama-antibodies asetshenzisiwe, yingakho ukukhetha i-IL yomuntu il - 2 Ukutholwa Kit Umhlinzeki noma umenzi kubalulekile. Abakhiqizi abadumile baqinisekisa ama-antibodies ahlolwa ngokuqinile futhi aqinisekise ukuhlinzeka ngemiphumela ethembekile.
● Ukuqina kombala kanye ne-IL - 2 Ukulinganiswa
Itshe le-IL ye-IL - amazinga ama-2 kumasampula asebenzisa ama-Kits okutholwa ukuqina kombala okukhiqizwe yi-enzyme - ukusabela okungaphansi. Lolu shintsho lweColorimetric lulingana ngqo nokuhlushwa kwe-IL - 2 kusampula. Ngokukala ukumunca esikhungweni esithile, abacwaningi banganquma ngokunembile i-IL - 2 Ukuhlushwa kusetshenziswa ijika elijwayelekile elenziwe ngokugxila olwaziwayo.
Izicelo ze-IL - 2 ukutholwa
● Umthethonqubo wokuthuthukisa amaseli
IL - 2 ineqhaza eliyisisekelo ekuthuthukisweni nasekwandeni kwama-T cell, abalulekile ekuvikeleni okungaguquki. Ama-kits we-human Il - 2 Amakhithi wokutholwa abalulekile ekuphepheni izimpendulo ze-T cell, ukuhlinzeka ngokuqonda ekusebenzeni kwamasosha omzimba. Lokhu kunemiphumela hhayi yocwaningo oluyisisekelo kuphela kodwa futhi nokuthuthuka kwezindlela zokwelapha okuqondiswe emzimbeni - Ukuphazamiseka okuhlobene.
● Umdlavuza we-FuncywoSafapy kanye nempendulo yokuzivikela komzimba
Iqhaza le-Il - 2 ekuthuthukiseni izimpendulo zokuzivikela komzimba liye lahlanganiswa umdlavuza, ikakhulukazi ekwelashweni okuhlose ukukhulisa amandla okuvikezela ama-tumors. Ama-kits we-Human Il - 2 Amakhithi wokuthola ama-2
Sibutsetelo nge-IL - 2. Izinhlobo zeKit zokuthola
● I-ELISPOT KITS: Ukuzwela nendlela
I-ELISPOT (Enzyme - Okuxhunyiwe i-Immunopot) AS ASTS adume ngokuzwela kwawo, ekwazi ukuthola ama-cytokines ngakunye - I-Human Il - Ama-2 amakhithi we-ELISPOT awusizo ikakhulukazi ekuboneni i-IL - 2 Amaseli Okumfihlo ngaphakathi kwesibalo sabantu, enikeza ubungako bezimpendulo zokuzivikela komzimba. Lokhu kuzwela okuphezulu kubaluleke kakhulu ekucwaningweni kwe-immemmerological, ikakhulukazi lapho uthola izimpendulo eziphansi - izinga le-cytokine kuzilungiselelo ezahlukahlukene.
● Elisa kits: Amasu we-Assay
U-Elisa Kits kukhona umsebenzi wokutholwa kwe-cytokine, enikeza indlela eqinile futhi eqondile yokuthola i-IL eyengeziwe - 2 ngezinhlobo eziningi zesampula. I-Human Il - Ama-2 Elisa Kits ancanyelwa ukuthembeka kwawo kanye nokusebenzisa kalula, okwenza ukuba kube yindawo yokuhlala ephephe umsebenzi wokuzivikela, amandla okugomela, kanye ne-pathogenesies.
Izici ze-Elipot Kit nezinzuzo
● ama-microplate - I-ASAYS esekwe
Ifomethi ye-microplate yemininingwane ye-eLispot ivumela ukuphakama - Ukuhlaziywa kokusebenzisa, okuzuzisa ezifundweni ezinkulu. Umthamo wokuhlola amasampula amaningi ngasikhathi sinye uthuthukisa ukuqina kwezibalo futhi wehlise ukuhlukahluka, ukuhlinzeka ngemininingwane ethembekileko kuzo zonke izimo zokuhlola eziningi.
● Ukuzwela okuphezulu kwezimpendulo zemvamisa ephansi
Enye yezinto zokusukuma zamakhithi we-ELISPOT yikhono labo lokuthola izimpendulo ze-cell ezingavamile, ezibalulekile ekucwaningweni kwe-immunology lapho okuphansi - Lokhu kuzwela kwenza i-Elespot ibe yithuluzi elihle kakhulu locwaningo lokugomela kanye nokuthuthukiswa kwenoveli ye-AmmunoSapapies.
Kuchazwe indlela ye-Elisa Kit
● Icebo leSandwich ELISA
ISandwich ELISA yindlela evelele esetshenziswa kumuntu IL - 2 Ama-Kits atholwayo ngenxa yokucaciswa kwawo nokuzwela. Le ndlela ifaka ukubopha kokuqala kwe-IL - 2 kuya ku-antilody yokuthwebula, kulandelwa ukutholwa nge-enzyme yesibili - Ukuvuselela antibod
● Ukulinganisa il - 2 ku-plasma kanye nabaningi
I-Human Il - Ama-2 Elisa Kits asetshenziselwa kakhulu ukukala amazinga we-cytokine ku-plasma kanye nabaningili besiko le-cell. Amandla okuhlaziya iL - Ukuhlushwa okungu-2 Kulawa manzi kubalulekile ukuthola izimpendulo ezihlelekile kanye nezimpendulo zokuzivikela emzimbeni, ikakhulukazi ocwaningweni olugxile ezifweni lokuvuvukala kanye nokungenelela kokwelapha.
Imininingwane ye-Colorimetric Elisa Thola imininingwane
● Isinyathelo - Ngo - Inqubo Yesinyathelo
Inqubo ejwayelekile ye-Elisa eColorimetric ifaka uchungechunge lwezinyathelo eziqala ngokulungiswa kwesampula, kulandelwe ukufakwa ngamasoshabodiesibodies okuthwebula. Ngemuva kokugeza okuningi ukususa izinto ezingenampilo, i-antibody yokutholwa yengezwa. Isinyathelo sokugcina sibandakanya ukwengezwa kwe-substrate ephendula nge-enzyme ukukhiqiza ushintsho lombala olungalinganiswa, ukuhlinzeka ngedatha yenani ku-IL - amazinga ama-2.
● Ukuncishiswa kwesiko lesiko eliphakathi nendawo
I-IL ekhulisa i-IL - 2 Kuma-Cell Culture Magamers anikezela ngemininingwane ekukhiqizweni kwe-cytokine ngaphansi kwezimo ezahlukahlukene zokuhlola. Lokhu kubalulekile ezifundweni ezihlola izimpendulo zokuzijabulisa kwamaselula, lapho ukunquma amazinga e-cytokine kungasiza ekuqondeni kwezindlela zokusayina amaseli kanye nemiphumela yezidakamizwa.
Ukubaluleka kwe-IL enembile IL - 2 Ukulinganisa
● Ucwaningo kanye nemiphumela yezokwelapha
I-IL eqondile ye-IL - Ukulinganisa okungu-2 kuyisisekelo kuzo zombili izicelo zocwaningo nezicelo zemitholampilo. Ecwaningweni, kunika amandla okuqonda okunembile kwezimpendulo zokuzivikela emzimbeni, kwazisa ukuthuthukiswa kwama-amcynotherapies nemijovo. Ngokwenyama, il - amazinga ama-2 angasebenza njenge-biomaker yezifo noma impendulo yokwelapha, esiza ekuphathweni kweziguli kanye nokwenza kahle kokwelashwa.
● Umthelela ekufundweni kwe-cytokine nokwelashwa
Amandla okulinganisa iL - 2 inemthethweni kakhulu inemithelela enkulu yokutadisha nokwelashwa kwe-cytokine. Kusiza ukuqondisisa okujulile kwamanethiwekhi e-cytokine kanye nezindima zawo kwezempilo nezifo, ekugcineni kuqondiswa ukwelashwa okuhlosiwe kwe-cytokine okungaguqulwa ukusebenza kahle.
Izinselelo ku-IL - 2 Ukutholwa
● Ukuzwela kanye nezinkinga zokucacisa
Enye yezinselelo eziyinhloko ku-Il - Ukutholwa okungu-2 kuqinisekisa ukuzwela okwanele nokucaciswa. Ukuhlukahluka ekusebenzeni kwe-antibody kungaholela ekungathobeki, kugcizelele ukubaluleka kokukhetha abantu abathembekile be-IL - 2 Abakhiqizi bakwaKit bathola kanye nabahlinzeki abaqinisekisa ikhwalithi yemikhiqizo yabo.
● Ukuhlukahluka ekuphatheni isampula
Enye inselelo ebalulekile ukuhluka okwethulwe ngesikhathi sokuphathwa kwesampula nokulungiselela, okungathinta ukunemba kanye nokuvela kwemiphumela. Amaphrothokholi kanye nokuqeqeshwa okujwayelekile kubalulekile ukunciphisa lezi zinkinga, uqinisekise ukuthi idatha etholwe kumuntu il - 2 Amakhithi wokuthola aqinile futhi athembekile.
Amathemba esikhathi esizayo ku-IL - 2 Amakhithi wokuthola
● Intuthuko yezobuchwepheshe
Ikusasa le-Human Il - Ama-kits okutholwa 2 athembisa, ngentuthuko yezobuchwepheshe ethuthukisa ukuzwela kwawo, ukucaciswa, nokusebenziseka kalula. Izinto ezintsha ezinjengezindawo eziningi ze-Multiplex ne-Point - zohlolo lokunakekelwa kungenzeka ukukhulisa ukusetshenziswa kwalezi zikhithi, ukuzenza zifinyeleleke kalula futhi zisebenze ngezilungiselelo ezahlukahlukene.
● Izicelo ezibanzi kanye nezinto ezintsha
Njengoba ukuqonda kwethu i-IL - 2 Biology kuyanda, ngakho-ke kuzokusebenzisa izinhlelo zokusebenza ze-IL - 2 Amakhithi wokuthola. Amasu wokwelapha amanoveli aqondiswe yi-IL - 2 Izindlela ezisezingeni eliphakeme, ziguqula izinketho zokwelashwa zezifo ze-autoimmune, umdlavuza, nangale kwalokho. Ukuqanjwa okuqhubekayo okwenziwa yi-Human Il - 2 Abakhiqizi bekhithi yokuthola amakhithi nabathengisi bazobe bebalulekile ekuhlangabezaneni nalezi zidingo ezivelayo.
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Isikhathi Sokuposa: 2024 - 12 - 09 15:26:03
