Ukuqalisa
I-genomic DNA Extraction iyinqubo esesisekelo e-molecular Biology, edlala indima ebalulekile ezinhlobonhlobo zocwaningo kanye nezicelo zezokwelapha. Ukuthuthukiswa kweI-genomic DNA Extraction KitI-S iguqule le nqubo, okwenza ukuthi ifinyeleleke, isebenze kahle futhi ithembeke. Le ndatshana idlulela emandleni okuhlanza kwe-genomic DNA, ukuhlola izinhlobo ezahlukene zesampula, izinqubo zokuhlanza, izinselelo, izicelo, ukuhlolwa kwekhwalithi, ezenzakalelayo, amakhithi wezentengiselwano. Ukugcizelelwa okukhethekile kubekwa ngokubaluleka kokwelashwa kwe-genomic DNA Kits. Noma ngabe ungumcwaningi, udokotela, noma uchwepheshe welebhu, lo mhlahlandlela ophelele uzonikeza ukuqonda okubalulekile ezintweni ezibonakalayo nasekuthuthukisweni kokukhishwa kwe-genomic DNA.
Isingeniso kuma-DNA ahlanza kits
● Ukubuka konke kwamakhithi wokuhlanza ama-DNA
Amakhithi we-genomic DNA akhipha amakhithi amathuluzi abalulekile kuma-molecular biology gameratories. Lawa ma-Kits enza lula inqubo yokukhishwa, eqinisekisa ukuphakama - I-DNA Yekhwalithi itholakala ezinhlotsheni ezahlukahlukene zesampula. Bahlinzeka nge-protocol evamile, benciphisa ukuhlukahluka kanye nokwenza ngcono ukuvela kabusha. Kutholakala kumafomethi amaningi, lawa ma-kits anakekela osayizi abahlukahlukene besampula nezidingo zokufaka.
● Ukubaluleka ezicelo ezahlukahlukene
Amakhithi wokuhlanza ama-DNA asemqokani ocwaningweni lwezakhi zofuzo, ukuhlonza kwemitholampilo, ama-forensics, kanye nezifundo zemvelo. Ocwaningweni, banika amandla isifundo sezinto zofuzo, ukuvezwa kofuzo, nokuguqulwa kwe-epigenetic. Ezimweni zemitholampilo, zisetshenziselwa ukuxilonga ukuphazamiseka kofuzo, ukuqapha ukusebenza ngempumelelo kokwelashwa, kanye nokwakha umuthi owenziwe ngezifiso. Ukwethenjwa kanye nokusebenza kahle kwalezi zikhithi kubaluleke kakhulu ngokukhiqiza imiphumela enembile nezezimali.
Izinhlobo zamasampula we-DNA Ukuhlukaniswa
● Amangqamuzana amhlophe egazi
I-genomic DNA ingakhishwa kahle kumangqamuzana amhlophe egazi asebenzisa amakhithi akhethekile. Lokhu kuwusizo ikakhulukazi ekuhlolweni kwemitholampilo kanye nokuhlolwa kofuzo, lapho amasampula egazi aqoqwa khona njalo.
● Amaseli esiko amathiphu
Amangqamuzana amasiko wezicubu anikeza indawo elawulwayo yokutadisha izinto zofuzo. Ukukhishwa kwe-DNA kulawa maseli kubalulekile ukucwaninga nge-biology yamaselula ne-molecular. Ama-Kits aklanyelwe amaseli wesiko lezicubu aqinisekisa isivuno esikhulu nobumsulwa.
● Izicubu zezilwane nezitshalo
Ukukhipha i-DNA kusuka ezithweni zezilwane nezitshalo zibeka izinselelo ezihlukile ngenxa yokuba khona kwama-inhibitors nezinhlaka eziyinkimbinkimbi zamaselula. Amakhithi we-DNA akhethekile we-DNA aklanyelwe ukuphatha lezi zinkimbinkimbi, eqinisekisa i-lysis efanelekile nokuhlanzwa.
● imvubelo namagciwane
I-Yeast nama-bacterial amaseli anokuphanga amaseli aqinile adinga izindlela ezithile ze-lysis zokukhishwa kwe-DNA okusebenzayo. Ama-kits ahambisana nalawa ma-microorganisms asebenzisa amasu we-enzymatic noma ama-mehendi okukhipha okuphezulu - ikhwalithi ye-genomic DNA.
Isinyathelo - Ngo - Inqubo Yokuhlanza Isinyathelo se-DNA
● Ukulungiswa kwesampula kokuqala
Isinyathelo sokuqala ekuhlanzeni kwe-DNA sihilela ukuqoqa nokulungiselela isampula. Lokhu kungafaka phakathi kwe-centrifugation, ukugeza kanye ne-homogenization, kuya ngohlobo lwesampula. Ukulungiswa kwesampula okufanele kubalulekile ukuze kukhulise isivuno se-DNA nekhwalithi.
● ysis kanye nezigaba ezibophayo
Isiteji se-lysis sibandakanya ukwehlisa ulwelwesi lwamaseli nokukhipha i-genomic DNA kwikhambi. Lokhu kufinyelelwa kusetshenziswa ama-detegents, enzyme, noma izindlela zemishini. Isiteji esibophayo siyalandela, lapho i-DNA iboshelwe khona esigabeni esiqinile, njenge-Silica, ngaphansi kwezimo zikasawoti eziphakeme.
● ukugeza kanye nokuqedwa izinyathelo
Izinyathelo zokuwasha zisusa ezingonakaliso kanye nokungcola, ukuqinisekisa ubumsulwa be-DNA. Ama-Buffers amaningi okugeza asetshenziselwa ukuqeda amaprotheni, ama-lipid, nezinye izichilo zeselula. Ekugcineni, isinyathelo sokuqedwa sikhipha i-DNA ehlanziwe esigabeni esiqinile sisebenzisa i-buffer noma amanzi aphansi.
Isixazululo - Izindlela ezenzelwe ukwehlukaniswa kwe-DNA
● Qhathanisa nezinye izindlela
Isixazululo - Izindlela ezenzelwe esekwe, njenge-phenol - ukukhishwa kwe-chloroform kanye nosawoti, yizindlela zesiko zokuhlanzwa kwe-DNA. Ngenkathi esebenza ngempumelelo, basebenza kanzima - ban kakhulu futhi badinga amakhemikhali ayingozi. Ngokuphambene
● Izinzuzo zesisombululo - indlela esekwe
Isixazululo - Amakhithi we-DNA asuselwa ku-DNA ahlinzeka ngezinzuzo eziningana, kufaka phakathi isivuno esikhulu se-DNA, ubumsulwa, kanye nokuvela kabusha. Zenzelwe ukunciphisa ukungcoliswa futhi kungalinganiswa kalula ukuthola okuphezulu - Ngaphezu kwalokho, azidingi amakhemikhali ayingozi, abenze baphephe abasebenzi belebhu.
Izinselelo Ekuhlanjweni Kwe-DNA
● Izinkinga ezijwayelekile ezihlangabezana nazo
Ukuhlanzwa kwe-DNA kungabhakelwa ngezinselelo, njengokuvunwa okuphansi, ukungcoliswa, kanye nokuwohloka. I-inhibitors ekhona kusampula ingaphazamisa izinhlelo zokusebenza ezansi, futhi ukuphathwa okungafanele kungaholela ekunikezelweni kwe-DNA.
● Izeluleko Zokuxazulula Izinkinga
Ukubhekana nalezi zinselelo, kubalulekile ukulandela i-KIT Protocol ngokucophelela. Kusetshenziswa ama-reagents amasha, kugcina imvelo yokusebenza ehlanzekile, nokuqinisekisa ukulungiswa kwesampula okufanele kunganciphisa izingqinamba eziningi. Ngaphezu kwalokho, okufaka izinyathelo zokulawula ezisezingeni, njengokulinganisa i-DNA ukugxilisa kanye nobumsulwa, kungasiza ekuboneni nasekuxazululeni izinkinga kusenesikhathi.
Izicelo ocwaningweni nomuthi
● Sebenzisa ucwaningo lwezakhi zofuzo
Ukukhishwa kwe-genomic DNA kuyinto ebalulekile ocwaningweni lwezakhi zofuzo, okunika amandla izifundo ngomsebenzi wohlobo, umthetho kanye nokuxhumana. Ixhasa amasu anjenge-PCR, ukulandelana, kanye nokuhlelwa kwe-genotyping, okuyisisekelo ekuqondeni izindlela zofuzo kanye nokuthuthukisa amasu amasha okulapha.
● Izicelo ekuxilongayo emtholampilo
Ekuhlolweni kwemitholampilo, ukuphakama - I-Chose genomic DNA ibalulekile ekuhlolweni kwezifo ezinembile, i-pronyos, kanye nokuqapha ukwelashwa. Izicelo zifaka phakathi ukuhlolwa kofuzo zezinkinga ezitholakala njengefa, i-Cancer Genomics, kanye nokutholwa kwezifo ezithathelwanayo. Ukwethenjwa kwamakhithi we-DNA akhipha amakhithi kubalulekile ukuhlinzeka ngolwazi olunembile futhi olufika ngesikhathi.
Ikhwalithi nesivuno se-DNA ehlanziwe
● Izici ezithinta ikhwalithi ye-DNA
Kunezici eziningana zithonya ikhwalithi kanye nesivuno se-DNA ehlanziwe, kufaka phakathi uhlobo lwesampula, indlela yokukhishwa, kanye nezinqubo zokuphatha. Ukuba khona kwama-inhibitors, njengamaprotheni nama-polysaccharides, kungathinta izinhlelo zokusebenza ezaphansi.
● Izindlela zokukala ukuhlanzeka kwe-DNA kanye nokuhlushwa
Ukuhlola ubumsulwa kanye nokuhlushwa kwe-DNA kubalulekile ekuqinisekiseni ukufaneleka kwayo kwezicelo eziphansi. Izindlela ezijwayelekile zifaka ukuhlaziywa kwe-spectrophotometric, ezihlinzeka ngezilinganiso zokungenisa (A260 / A280) ukuhlola ubumsulwa, kanye ne-fluorometric amehlo, enikeza izilinganiso zokuhlushwa ezibucayi nezinembile.
I-automation kanye nokuhlanzwa okuphezulu kwe-DNA
● Technologies evumela izinqubo ezenzakalelayo ezenzakalelayo
I-automation ekuhlanzeni kwe-DNA ibaluleke kakhulu ekusingatheni amavolumu amakhulu empumulo kanye nokwenza ngcono ukusebenza kahle komsebenzi. Izinhlelo ezenzakalelayo, ezifana nabaphathi bama-robotic ketshezi namapulatifomu ezenzakalelayo azenzakalelayo, asuse inqubo, anciphise iphutha lomuntu, futhi andise futhi.
● Izinzuzo zezisombululo eziphakeme zokudlula
Izisombululo eziphezulu zokuhlanza i-DNA zibalulekile ukuze zikhulu - ukukala izifundo zofuzo, ukuhlonza kwemitholampilo, ne-biobanking. Banika amandla ukucubungula kanyekanye kwamasampula amaningi, ukonga isikhathi nezinsizakusebenza. Izinhlelo ezenzakalelayo futhi zinikeza imiphumela engaguquki, ukuthuthukisa ukuvela kabusha kanye nokwethenjwa.
Ukuhlaziywa kokuqhathanisa kwamakhithi okuhweba
● Ukuqhathanisa ukusebenza kwamakhithi athandwayo
Kunamakhithi we-genomic DNA akhipha amakhithi atholakala kubakhiqizi abahlukahlukene, ngalinye linezici ezihlukile kanye nezimpawu zokusebenza. Izici okufanele zicatshangelwe lapho ukhetha ikhithi faka isivuno, ubumsulwa, ukusebenziseka kalula, nezindleko. Izifundo zokuqhathanisa nokubuyekezwa komsebenzisi zinganikeza ukuqonda okubalulekile ekusebenzeni kwamakhithi ahlukene.
● Ukubuyekezwa kwabathengi kanye nemibono yesazi
Impendulo Yomsebenzisi kanye nokubuyekezwa kwesazi kudlala indima ebalulekile ekuhlolweni kokusebenza kwe-Genomic DNA Kits. Ukubuyekezwa okuhle nokuvumelanisa kusuka emithonjeni ehlonishwayo kungaqondisa abacwaningi ekukhetheni ikhithi engcono kakhulu yezidingo zabo. Ngokwengeziwe, impendulo evela kubasebenzisi isiza abakhiqizi ukuthi bathuthukise imikhiqizo yabo futhi babhekane nanoma yiziphi izingqinamba.
Izitayela zesikhathi esizayo kubuchwepheshe bokuhlanza kwe-DNA
● Ubuchwepheshe obusafufusa ekukhishweni kwe-DNA
Inkambu yokuhlanza kwe-DNA iyavela ngokuqhubekayo, ngobuchwepheshe obusha obuqhamukayo ukubhekana nemikhawulo yamanje. Ukusungulwa okufana nama-microfluidics, i-nanotechnology, kanye ne-magnetic bead - Ukukhishwa okusekelwe kudalula indlela yokuthola izindlela ezisebenza kahle nezinembile ze-DNA.
● Izibikezelo zentuthuko yesikhathi esizayo enkundleni
Izitayela zesikhathi esizayo ekuhlanzeni kwe-DNA kungenzeka ukuthi zigxile ezenzakalelayo, ezincane, kanye nokuhlanganiswa namanye amasu we-molecular biology. Ukuthuthukiswa kwamakhithi wendawo yonke okungaphatha izinhlobo ezahlukahlukene zezinhlobo zemibandela kanye nezimo kulindeleke ukuthi zithuthukise ukuguquguquka kanye nokuguquguquka kokusebenza kwezindawo zokuhamba phambili kwe-DNA.
I-Bluekit: Ukuphayona kwe-DNA Ukukhishwa kwezixazululo
Jiangsu Hillgene, ngaphansi kwegama lomkhiqizoI-Bluekit, uzisungule njengomdlali oholayo emkhakheni wokukhishwa kwe-genomic DNA. Itholakala eSuzhou, ngeziza zokukhiqiza eShenzhen naseShanghai, kanye nesiza esizayo eNorth Carolina, eHillene inikezela ngesimo - of Art DNA Eltoction Solutions. Imikhiqizo ye-Bluekit yenzelwe ukufeza izidingo eziqinile zokwelashwa kwamaselula, isekela ukuthuthukiswa kwemoto - t, tcr - t, iseli elisekelwe. Ngokuzibophezela kokusungula izinto ezintsha kanye nekhwalithi, iHillgene kunikezelwe ukuthuthukisa inkambu yokwelashwa kwamaselula kanye nokwenza ngcono imiphumela yeziguli.
Ngamafuphi, ukuhlanzwa kwe-genomic DNA kuyinqubo ebalulekile kwi-biology ye-biology, ebanzi - ukusukela ngezicelo ocwaningweni nomuthi. Intuthuko kumakhithi wokukhishwa kwe-DNA, okubandakanya naleyo bluekit, yenze le nqubo isebenze kahle, ithembekile, futhi ifinyeleleke. Njengoba ubuchwepheshe buyaqhubeka nokuvela, ikusasa lokuhlanza kwe-DNA liphethe amathuba athokozisayo okuthuthukisa ukutholakala kwesayensi kanye nokwenziwa kwemitholampilo.
Isikhathi Seposi: 2024 - 09 - 27 14:40:06
