Ukuqalisa
● Ukubaluleka kokukhishwa kwe-genomic DNA
Esikhathini se-realm ye-molecular biology, ukukhishwa kwe-genomic DNA kuyinqubo eyisisekelo ebeka isisekelo se-MyRIAD yezinhlelo zokusebenza, kusuka ocwaningweni kuya kokuxilongwa kwemitholampilo nomuthi owenziwe ngezifiso. Inqubo ye-genomic DNA ye-Extraction ifaka ukwehlukanisa i-DNA kusuka kumaseli ukuze ihlaziye futhi ikhokhise izinto zofuzo, ukuhlinzeka ngokuqonda okubalulekile ezifweni zofuzo, i-biology yemvelo kanye ne-biotechnological athuthukayo. Ngesidingo sokujikijela izifundo ze-genomic, indima yethembekileI-genomic DNA Extraction Kitayikaze ibe yinto ebaluleke kakhulu. Lawa ma-kits ahambisa inqubo ye-extraction, aqinisekise isivuno esikhulu kanye nobumsulwa be-DNA, ebalulekile kwizicelo ezaphansi.
Amasu wokuqoqa amasampula
● Izinhlobo zamasampula wokukhishwa kwe-DNA
Ubuqotho be-genomic DNA buqala ngeqoqo eliphakeme - amasampula wekhwalithi. Imithombo ejwayelekile ifaka igazi, izicubu, amathe, kanye nama-buccal swabs. Uhlobo ngalunye lwesampula lunikeza izinzuzo ezihlukile; Isibonelo, amasampula egazi acebile ku-DNA kodwa adinga ukuphathwa ngokucophelela, ngenkathi amathe kanye ne-buccal kanye ne-swabs enikeza okungeyona - izinketho ezihlaselayo. Ukukhetha isampula efanelekile kubalulekile, ikakhulukazi uma usebenzisa i-cell therapy genomic DNA Extraction Kit, okungenza kahle izinhlobo ezithile zeseli, ziqinisekisa isivuno esikhulu kanye nobumsulwa.
● Imikhuba emihle kakhulu yokuphatha isampula
Ukuqinisekisa ukuthembeka kwesampula kubalulekile ekukhishweni kwe-DNA okuphumelelayo. Amasampula kumele aqoqwe ekungcolisweni - Imvelo Yamahhala, egcinwe emazingeni okushisa afanele, futhi aphathwe ngokunakekela ukuvikela ukonakaliswa. Lesi sinyathelo sibalulekile ukuthi ngabe ubhekene nezinhlamvu ezintsha noma lezo ezigcinwe isikhathi eside, njengoba noma yikuphi ukonakaliswa kungahlehlisa isivuno sokugcina se-DNA nekhwalithi, kuthinta imiphumela yokuhlola.
Izindlela ze-Cell Lysis
● Amasu amakhemikhali akwakheshi we-vs
Isinyathelo se-Cell Lysis siyinto ebalulekile ekudedeleni i-genomic DNA kwizakhiwo zeselula. Ukuhlolwa kwamakhemikhali, okubandakanya okokuhlanza kanye nama-enzyme, kwephula kahle ulwelwesi lwamaseli namaprotheni. Izindlela ezibonakalayo - njengokuphazamiseka kwemishini kanye ne-sonication - zinamandla amakhulu futhi zingasetshenziselwa amasampula aqinile. Ukukhetha kwendlela kuvame ukuncika ohlotsheni lwesampula kanye nezidingo ezithile ze-genomic DNA Extraction Kit ekusetshenzisweni. Abakhiqizi Abakhiqizi Kits ukwengeza ukusebenza kahle kwe-lysis, ukulinganisa phakathi kokulula nokusebenza ngempumelelo.
● Ukubaluleka kwe-lysis ekukhishweni kwe-DNA
Ukuhlolwa okusebenzayo kuqinisekisa ukuthi izakhi zeselula zihlukaniswe ngokwanele, zisiza ukukhishwa kwe-DNA ye-Itact kukhambi. I-lysis eyenziwe kahle i-lyrincizes ishelele i-DNA kanye nokungcoliswa kwamaprotheni nama-lipids, okubaluleke kakhulu kwizicelo ezifana nokwelashwa kwamaseli, lapho ubuqotho be-genomic bufana khona.
Ukususwa kwamaprotheni nokungcola
● Indima ye-solvents ekuhlanjululweni
Ukuthunyelwe - Ukuguqulwa, ukususwa kwamaprotheni nokunye ukungcola kubalulekile. I-Sol sol enjenge-phenol - i-chloroform ngokwesiko isetshenziswa ngokwesiko futhi ihlukanise amaprotheni avela kuma-nucleic acid. I-genomic DNA ye-DNA DNA ama-KNA ajwayele ukusebenzisa i-silica - ama-memperos asuselwa ku-mentbranes noma ubuhlalu bakazibuthe, obubopha i-DNA ukukhetha ngenkathi uvumela ukungcola kuhlanzwe. Lesi sinyathelo sibalulekile ekutholeni i-DNA ehlanzekile efanelekile ukuthola izinhlelo zokusebenza eziqondile nezibucayi.
● I-centrifugation ichaziwe
I-CentriFugation isinyathelo esivamile sokuhlukanisa i-DNA kusuka ekungcoleni okuthunyelwe - lysis. Ngokusebenzisa amandla amakhulu e-centrifugal, imfucumfucu yamaselula namaprotheni amakhalekhukhwini, ngenkathi i-DNA ihlala isensana. Lesi sinyathelo sivame ukuphindwa nge-tandem ngezinyathelo zokuhlanza ukuze uthuthukise ubumsulwa kanye nokuhlushwa kwe-DNA ekhishwe.
Inqubo ye-DNA Preacitation
● Ukusetshenziswa kwe-isopropanol ne-ethanol
I-Precipitation yindlela ye-classic yokubuyisa i-DNA kusuka kusixazululo, kuxhashazwa cishe kuwo wonke amakhithi we-genomic DNA akhipha. Izimo ezifana ne-sopropanol ne-ethanol zinika amandla i-DNA ukunciphisa ngokunciphisa i-solubity yayo. Lapho sengibekile, amafomu e-DNA abonakalayo ama-clumps noma ama-filaments, ahlinzeka nge-cue ebonakalayo yokukhishwa ngempumelelo.
● Ukubona ngeso lengqondo amafayela we-DNA
Ukuba khona kwama-filaments we-DNA kuqinisekisa ukuhanjiswa kwezulu okusebenzayo futhi kusebenza njengenkomba yokuqala yempumelelo yokukhishwa. Lesi sinyathelo, yize sibonakala sisisekelo, sibalulekile ukuzethemba kwinqubo yokukhipha, uqinisekise ukuthi i-DNA eyanele ikhona ekuhlaziyeni okulandelayo.
Ukuwasha kwe-DNA
● Ukubaluleka kwe-ethanol ukugeza
Ukuwasha kwe-DNA pellets nge-ethanol kusebenza ukususa usawoti osele nokungcola. Lesi sinyathelo, yize sabonakala sisejwayelekile, kubalulekile ekuvikeleni ukungcoliswa okungathinta izinqubo ezaphansi. Ukuwasha kwe-Ethanol kuchazwe ngokufanele kuyo yonke incwajana yabasebenzisi nge-genomic DNA Extraction Kit Accurents Abakhiqizi be-Genomic DNA Kit ukuqinisekisa ukuthi abasebenzisi bafeza imiphumela efanele.
● Ukuqinisekisa ukuhlanzeka kwe-DNA
Ukuhlanzeka kubalulekile njengesivuno ekukhishweni kwe-DNA. Ukungcola okusele kungavimba ukusabela kwe-enzymatic ngezicelo ezaphansi ze-Downstream ezifana ne-PCR nokulandelana. Abahlinzeki be-genomic DNA Kit Devil bagcizelela ubumsulwa, ukuhlinzeka ngezimiso nezinto zokwakha eziqinisekisa ukuthi zingcolisiwe zigezwe kahle.
Ukuncibilika nokulungiselela i-DNA
● Ukukhetha i-buffer efanele
Lapho i-DNA ihlanzwe, ichithwa ku-buffer efanelekile, imvamisa i-buffer noma amanzi afakwe emanzini. Ukukhethwa kwe-buffer kungathinta ukuqina nobuqotho be-DNA, kuthonya isikhathi eside sesikhathi eside - I-Genomic DNA Extraction Kit Factory Factorict ivame ukuhlinzeka ngama-buffers ahlelwe ngezicelo ezithile ezaphansi.
● Ukulungiselela i-DNA ukuthola izivivinyo
Ukulungiswa okufanele kwe-DNA kubalulekile empumelelweni yokuhlola, ukuqinisekisa ukuhambisana namasu wokuhlaziya afana ne-QPCR, okulandelayo - Izindlela zokulungiselela kahle zandisa ubuqotho be-DNA nokusebenza kwalezi zicelo.
Ukulawulwa kwekhwalithi ye-DNA ekhishwe
● Amasu wokuhlola ikhwalithi ye-DNA
Ngemuva kokukhishwa, ukuhlola ikhwalithi ye-DNA kubalulekile. I-Spectrophototometry iyindlela edumile, ukukala umuzwa ngo-260 NM futhi unikeze imininingwane mayelana nokuhlushwa kwe-DNA kanye nobumsulwa. Ngokwengeziwe, i-Electrophoresis ye-GEL ivumela ukubona ngeso ubuqotho be-DNA, ukuthola noma yikuphi ukonakaliswa.
● Ukuqonda Ukufundwa Kwe-Spectrophotometer
Ukufundwa kwama-spectrophotomemeter kunikeza ukuqonda ocingweni nasekuhlanzeni kwe-DNA. Isilinganiso esingu-260/880 esiseduze ne-1.8 sibonisa i-DNA Ehlanzekile, ngenkathi ukuphambuka kuphakamisa ukungcoliswa. Lokhu kufundwa kubaluleke kakhulu ekuqinisekiseni ukuthi i-DNA ilungele izinhlelo zokusebenza ezibucayi phansi.
Izinselelo Ekukhishweni kwe-DNA
● Izinkinga ezijwayelekile kanye nokuxazulula inkinga
Naphezu kwentuthuko, izinselelo ekukhishweni kwe-DNA ziphikelela, kufaka phakathi isivuno esiphansi, ukungcoliswa, kanye nokonakaliswa. Ukuqonda lezi zinkinga nokulandela imihlahlandlela yokuxazulula izinkinga - evame ukuhlinzekwa ngabakhiqizi - kubalulekile ekukhipheni okuphumelelayo.
● Ukuhluka ngezinhlobo ezihlukile zesampula
Izinhlobo zesampula ezahlukahlukene zibonisa izinselelo ezihlukile ekukhishweni kwe-DNA, njengokuqukethwe kwe-DNA okuguqukayo nokuba khona kwama-inhibitors. I-Genomic DNA Extraction Kit Suppliers Design Kits ukubhekana nalokhu kwehlukahluka, okunikeza izisombululo ezenziwe ngezezimali zesampula ezemvelo.
Intuthuko kubuchwepheshe bokukhishwa kwe-DNA
● Izinto ezintsha kumakhithi wokukhishwa
Intuthuko eqhubekayo kuma-genomic DNA Extraction Kits enziwe lula inqubo, ukuthuthukisa ukusebenza kahle, isivinini, kanye nokwethembeka. Lezi ezintsha zifaka ezenzakalelayo, ubuchwepheshe be-magnetic bead, kanye nokuhlanganiswa namarobhothi ezicelo zokusebenzisa okuphezulu, okubonisa izidingo ezivelayo zocwaningo lwe-genomic.
● Izitayela zesikhathi esizayo kwisayensi ye-genomic
Njengoba i-genomic Sciences Advance, isidingo sokuphakama - Isizinda se-DNA sekhwalithi sizokhula. Izitayela zesikhathi esizayo zifaka ezinye izindlela ze-Eco - Izindlela zokukhishwa ezinobungane, ezenzakalelayo, kanye nokuhlanganiswa kokukhishwa kobuchwepheshe obulandelayo bokuhlaziya, kuveze indlela yokuhlaziya okusebenzayo nokuphelele kwe-genomic.
I-Bluekit: Iphayona ngezixazululo zokwelapha ze-genomic nasemafeyili
Jiangsu Hillgene, ngaphansi komkhiqizoI-Bluekit, ibeka phambili phambili kwe-genomic kanye ne-cellular therapy emisha. I-Headquarter eSuzhou ngezindawo ezingeziwe eShenzhen naseShanghai, kanye nesiza esizayo eNorth Carolina, eHillinglene inweba ukufinyelela emhlabeni jikelele. I-Bluekit®Imikhiqizo ihlinzeka ngezixazululo eziphelele zokulawulwa kwekhwalithi ekwelashweni kweseli, ukusekela ukuthuthukiswa kwezindlela zokwelapha ezithuthukile ezifana nemoto - t ne-TCR - T. Ngokusebenzisa amapulatifomu azinikezele ezokukhiqiza i-nucleic acid kanye ne-QC, i-Bluekit iguqula isimo sokwelashwa kwamaselula, silethe imikhiqizo eguqukayo ukuze zimake ngokushesha nangendlela efanele.
Isikhathi Sokuthumela: 2024 - 12 - 05 15:07:02
