Isingeniso ku-Gene Copy Inombolo yokutholwa
Ku-ESTER ESS - Ukuvela Kwezwe Yocwaningo Lwafuzo, ukuqonda izinombolo zohlobo lwezakhi zofuzo sekuyitshe lezicelo zombili zokuxilonga nezokwelapha. Ukubaluleka kwe-Gene Copy Inombolo yokuhlukahluka (CNVS) akunakweqiwa, njengoba kubalulekile ekufundeni ukuphazamiseka kofuzo, ukuqhubeka komdlavuza kanye nokusebenza kwezindlela zokwelapha zofuzo. Phakathi kofuzo oluningi lwenzalo, i-RCL gene kanye nohlobo lwe-vsvg luvelele izindima zabo ezinqubweni zamaselula kanye nobunjiniyela be-viral veric. Le ndatshana idlulela emhlabeni we-Gene Copy Inombolo Detection, egxile ekubukeni okufana neI-Gene Cop Inombolo Kitkanye nokwelashwa kwamaselula kokwelashwa kwenombolo yenombolo, konke kulethwe kuwe ngokuhola izinombolo zohlobo lwenombolo ye-gene.
Ubuchwepheshe obuthuthukisiwe ocwaningweni lwezakhi zofuzo
● Indima ye-QPCR in dese
I-Polymerase Chain reaction (QPCR) ivele njengethuluzi elisemqoka ekunciphisweni kwezinombolo zohlobo lweGene. Le ndlela ivumela ukukhuliswa okunembile kanye nokuncishiswa kokulandelanisa kwe-DNA, okwenza kube kuhle ukuthola ama-CNV. Ngokwesimo sokwelashwa kwe-gene, lapho ukulethwa okunembile nokubonakaliswa kwezinhlobo zokwelapha kubaluleke kakhulu, Qpcrale
● Izinto ezintsha kubuchwepheshe be-Lentiviral Vector
Ukuqala kwe-Lentiviral Vectors kuguqule ukwelashwa kweGene, ukuhlinzeka ngezindlela ezifanele zofuzo kumaseli okuhlosiwe. Lawa ma-veector, avame ukuhlome ngohlobo lwe-vsvg, anwebelwe ukuthuthukiswa kokuguqulwa kokuguqulwa nokuqina. Ukuhlanganiswa kwenombolo ye-gene yohlobo lwe-gene Copy Copy ku-Lentiviral Vector Production kuqinisekisa ukuthi imithamo yofuzo ilawulwa ngokucophelela, okwenza imiphumela yokwelapha.
Sibutsetelo nge-RCL (VSVG) COME Copy Inombolo yenombolo
● Izici nezinzuzo zeI-BluekitUmphumela
I-RCL (VSVG) Inombolo yenombolo ye-RCL Copy Kit kusuka ku-Bluekit imele ukuthuthuka okukhulu ekuxilongeni kofuzo. Yakhelwe ukunemba, inikeza ukutholwa okusheshayo nokunembile kwezinombolo zohlobo lweGene Copy, kubhekise kuzo zombili izidingo zocwaningo nezidingo zomtholampilo. Iphrothokholi yayo ehleliwe kanye nomsebenzisi - Idizayini enobungane yenza kube yithuluzi elibalulekile lezinto zokucwaninga emhlabeni jikelele.
● Izingxenye ezibalulekile kanye nokusebenza
Enhliziyweni yomkhiqizo we-Bluekit isethi yayo ephelele yama-reagents kanye nezilawuli, ukuqinisekisa ukusebenza ngamandla. I-kit ifaka phakathi ijika elijwayelekile elilinganiselwe, okuvumela abaphenyi ukuthi bafeze ukucaciswa okunembile ngomzamo omncane. Njengomhlinzeki we-gene oholayo wenombolo ye-gene Copy Kit, i-Bluekit igcizelela ikhwalithi nokwethembeka eminikelweni yayo yomkhiqizo.
I-Fluorescent Probes kanye ne-Multiplex PCR ENDEDEOLK
● Ukuchazwa kwamasu we-fluorescent
Amasu we-Fluorescent Plooness asephenduke indinganiso yegolide ekulinganiseni i-Gene. Ngokusebenzisa ama-alews aphenya ama-fit floorescence ekwakheni kwe-hybridization kwi-DNA eqondiwe, lezi zinqubo zinikeza ukuzwela okuphezulu nokucaciswa, okubalulekile ukuthola ama-CNV acashile ezindaweni eziyinkimbinkimbi zofuzo.
● Izinzuzo ze-Multiplex PCR ekutholweni kofuzo
I-Multiplex PCR ithuthukisa ukusebenza kahle kokuhlolwa kwezakhi zofuzo ngokuvumela ukukhuliswa okukodwa kokuhlosiwe okuningi ngaphakathi kokuphendula okukodwa. Lokhu akugcini nje ngokulondolozwa kwesampula ebalulekile kodwa futhi kunciphisa isikhathi nezindleko ezihambisana nokuhlaziywa kofuzo, okwenza kube ukukhetha okuthandwa kakhulu phakathi kwabakhiqizi be-gene Copini.
Izicelo ku-Biomedical Research
● Sebenzisa umdlavuza kanye nezifundo zefuzo
I-Gene Cop Inombolo yokuhlukahluka idlala indima ebaluleke kakhulu kwi-pathogenesis yemidlavuza ehlukahlukene kanye nokuphazamiseka kofuzo. Ama-Kits afana ne-cell therapy gene Copy Inombolo yenombolo iyithuluzi ekuboneni lokhu kuhlukahluka, okunika amandla izindlela zokwelashwa ezenziwe kanye nokwenza ngcono imiphumela yeziguli.
● Imiphumela yokukhiqizwa kwe-vector viral
Ekukhiqizeni i-Veral Vector, uqinisekisa inombolo efanele ye-gene Copy ibalulekile ekusebenzeni kahle kokwelapha nokuphepha. Ukusetshenziswa kwe-gene Copy Inombolo Kits kule nqubo kuqinisekisa ukuthi ama-vevectors akhiqizwa ngokulayisha kofuzo okufunwayo, athuthukise amandla abo okulashwa.
Ukunemba nokusebenza kahle ekuxilongeni kofuzo
● Ukubaluleka kokunemba kokukopishwa kofuzo
Ukunemba ekukopekeni kwezakhi zofuzo kubalulekile kuzo zombili izicelo zocwaningo nasemitholampilo. Amaphutha ezithandweni zofuzo angaholela ekuhlolweni okungalungile noma ukwelashwa okungaphumeleli. Ukunemba okunikezwa yi-Advanced Gene Copy Inombolo Kits, njengaleyo evela kwa-Bluekit, iqinisekisa ukuthi ukuhlaziya izakhi zofuzo zombili zithembekile futhi zizakhiwe kabusha.
● Le khithi iqinisekisa imiphumela ephezulu yokunemba
Ngokuqamba okucacile nokuhlolwa okuqinile, Inombolo ye-BlueKit Copy Copy Popy iletha imiphumela ephezulu yokunemba. I-kit ngayinye yakhelwe ukunciphisa ukwehlukahluka futhi yandise ukunemba, ukusetha okujwayelekile kokukhophi kwenombolo kokhetho Kit Factory emhlabeni jit.
Umsebenzisi - ukwakheka okunobungane nezivumelwano ezihleliwe
● Kwenziwe lula izinqubo zokuhlola izakhi zofuzo
Ubunzima bokuhlolwa kwezakhi zofuzo buvame ukuba nezinselelo zabaphenyi. I-Bluekit ibhekana nalokhu nemikhiqizo engagcini ngokusika kuphela - onqenqemeni kodwa nangokwaziswa. Amaphrothokholi abo ahleliwe abe lula inqubo yokuhlola, evumela abaphenyi ukuthi bagxile emiphumeleni yokuhumusha kunokuba kuhlolwe izivivinyo zokuxazulula inkinga.
● Isikhathi - Ukusindisa Izici Zomkhiqizo We-BlueKit
Ukusebenza kahle kwesikhathi kuwuphawu lwe-Bluekit gene Copy Copy Kits. Ngokunciphisa inani lezinyathelo nokufaka ukulungele - uku- - Sebenzisa ama-reagents, lawa makhithi asindisa isikhathi esibalulekile nezinsizakusebenza, ukuzenza intandokazi phakathi kwama-gene Copini Inombolo yeKit Kit.
Isixazululo esiphelele ngejika elijwayelekile
● Izinzuzo zokuba nokwakhiwa - Ijika elijwayelekile
Ijika elakhiwe - Ijika elijwayelekile liyisici esibalulekile esibeka imikhiqizo ka-BlueKit. Iqinisekisa ukwakheka okungaguquki kuzo zonke izivivinyo, kunciphisa amandla ephutha lomuntu kanye nokwenza ngcono ukuthembeka kwemiphumela.
● Ukwehliswa kwephutha lomuntu nge-Pre - Setha Ukulinganiswa
I-Pre - Setha amapharamitha wokulinganisa asho ukuthi abasebenzisi bachitha isikhathi esincane sokulinganisa kanye nesikhathi esengeziwe esigxile ekuhumusheni kwedatha. Lokhu kuncishiswa kwephutha lomuntu kubalulekile ukuze kugcinwe ubuqotho bokuhlaziywa kofuzo.
Izifundo zamacala kanye noMhlaba - Izicelo zomhlaba
● Izibonelo zocwaningo oluphumelele usebenzisa ikhithi
Izifundo eziningi zokucwaninga zisebenzise ngempumelelo imikhiqizo ye-bluekit ye-bluekit ukuze ithuthukise ukuqonda kwabo izinombolo zohlobo lweGene. Isibonelo, izifundo zomdlavuza zakamuva zisebenzise lezi zikhithi ukuthola ama-CNV abucayi ahambisana nokuthuthuka kwezithuthuthu nokwelashwa.
● Ubufakazi obuvela kubacwaningi bezakhi zofuzo
Abaphenyi abaholayo ensimini baphinde badumisa amakhophi enombolo yofuzo kusuka ku-bluekit ngokunemba kwawo, ukusebenziseka kalula, kanye nokwethenjwa. Ukuvumela okunjalo kugcizelela isikhundla se-kits 'njengokukhetha okuholayo phakathi kwabakhiqizi be-gene Cop.
Izinkomba zesikhathi esizayo ekuhlaziyweni kwenombolo ye-Gene Copy
● Intuthuko engaba khona ekuhlolweni kofuzo
Ikusasa lokuhlolwa kwezakhi zofuzo lilele ekuhlanganiseni ubuchwepheshe obuyinkimbinkimbi kakhulu kanye nezindlela. Njengoba insimu ivela, i-Bluekit ilungele ukuhola le nkokhelo, ukuthuthukisa izixazululo ezintsha ezicindezela imingcele yalokho okwenzeka ekuhlaziyweni kwenombolo ye-Gene Copy.
● Izisombululo zika-bluekit ezingakwazi ukwakheka kanjani ucwaningo lwesikhathi esizayo
Ukuzibophezela kukaBluekit ekusukeni okusha kuqinisekisa ukuthi kuhlala kusengaphambili kocwaningo lwezakhi zofuzo. Ngokuphinda ucwilisa amakhophi abo e-gene copy
Mayelana ne-bluekit
UJiangsu Hillgene, usebenza ngaphansi komkhiqizo weBluekit, ubhekiswe eSuzhou ngezitshalo ze-GMP ebanzi nesikhungo se-R & D. Baziqhayisa ngezindawo zokukhiqiza eShenzhen naseShanghai, ngokuba khona okuzayo eNorth Carolina, e-USA. I-BlueKit isebenza ngokukhethekile emapulatifomu athuthukayo ngemikhiqizo yokwelashwa kwamaselula, exhasa imoto - t, tcr - t, kanye ne-stem cell ezintsha. Ukuzibophezela kwabo ekulawulweni kwekhwalithi kanye nokuthuthukiswa komkhiqizo weselula kubabeka kubo njengabaholi enkundleni, okunikezelwe ekuthuthukiseni ukwelashwa kwamaselula ngezinzuzo zesiguli emhlabeni jikelele.
Isikhathi sePosi: 2025 - 02 - 28 11:58:06
