Isingeniso se-E.Coli Residial DNA ukutholwa
● Ukubaluleka kwe-biotechnology
Endaweni eyinkimbinkimbi ye-biotechnology, ukutholwa kweI-DNA InsalelaS, ikakhulukazi i-E.Coli Residial DNA, ivela njengokukhathazeka okubucayi. Njengonjiniyela wofuzo kanye nokukhiqizwa kwe-biopharmaceutical ukuzuza, ukulondolozwa kobumsulwa bomkhiqizo kuba yinto enkulu. Izinsalela ze-DNA ezivela ezilwaneni ezisingathwa njenge-E.Coli zingahlala emikhiqizweni yokugcina, zibeka izingozi zemvelo futhi zibangele izimpendulo ezimbi zomzimba ezigulini. Lokhu kubaluleke kakhulu emasimini afana nokwelashwa kwamaseli, lapho ukungcoliswa kwe-DNA kunganciphisa ukusebenza kahle nokuphepha kwemikhiqizo yokwelapha.
● Ukuphepha nokuPhepha kokulandela
Uhlaka olujwayelekile lokulawula libonisa amazinga avumelekile we-DNA izinsalela, futhi abakhiqizi kumele babambe kule mihlahlandlela yokuqinisekisa ukuphepha komkhiqizo. Ukuba khona kwe-DNA izinsalela zemikhiqizo efana nemijovo, ama-antibodies e-monoclonal, kanye neseli - Izindlela zokwelapha ezisekelwe kungaholela ekukhunjuleni umkhiqizo futhi kubangethe ubungozi obukhulu bempilo. Ngakho-ke, izindlela ezintsha nezinokwethenjelwa zokuthola nokuthola lezinsalela zibaluleke kakhulu. Ngokukhuphuka kwesidingo sokwelashwa kwamaselula, ukutholwa kwensalela ye-DNA kuyisinyathelo esibalulekile ekulawulweni kwekhwalithi, kuqinisekisa ubumsulwa kanye nokuphepha kokungenelela kokwelapha.
Ukuqonda Umgomo We-Taqman Probe
● inqubo yokutholwa kwenani
Ukuqala kweTaqman Profee Technology kuguqulwe ukutholwa kwe-DNA. Le ndlela isebenzisa uphenyo olubhalwe ngobuqili olwenziwe nge-hybridizes ekulandeleni okuthile kwe-DNA, lula kwangempela - Lapho uphenyo lubopha kwi-DNA yalo kwelitshe ngesikhathi se-PCR Amplification, kukhishwa isibonakaliso se-fluorescent, esilingana nenani le-DNA ekhona kusampula.
● Indima ekutholeni i-E.Coli DNA
Ama-taqman kungenzeka aphenya ngokukhethekile ukuthola izinsalela ze-E.Coli DNA ngenxa yokucacisa kwabo okuphezulu nokuzwela. Lezi zingcweti zingahlukanisa phakathi kokulandelana okuhlobene ne-DNA, ukuqinisekisa ukutholwa okunembile nokuncishiswa. Ukuqina kweTekhnoloji kwenza kube yithuluzi elisemqoka labakhiqizi be-DNA abasele, abahlinzeki, kanye namafektri okuhlose ukugcina amazinga aphezulu okuphepha nokuhambisana nemikhiqizo yabo.
Izinzuzo zokusebenzisaI-BluekitIkhithi yokuthola
● Yisho - Kwe - The - Izici Zobuchwepheshe Zobuchwepheshe
I-Bluekit's Detection Kit ifaka i-Adgems yakamuva kuTaqman Technology Technology, enikeza ukuzwela okungenakuqhathaniswa nokunemba ekutholeni izinsalela ze-E.Coli DNA. Idizayini kaKit iqinisekisa isiphambano esincane - Ukuvuselelwa kanye nokucaciswa okuphezulu, okubalulekile kwemiphumela ethembekile kumasampula ayinkimbinkimbi wemvelo. Lokhu kusika - Isixazululo Enqenqemeni senzelwe abahlinzeki be-DNA abasele kanye nabakhiqizi abafuna ukunemba nokuvumelana nezinqubo zabo zokutholwa.
● Ukuzwela okungenakuqhathaniswa nokucaciswa
I-Bluekit Detection Kit imele amandla ayo okuthola amazinga aphansi we-DNA izinsalela, ukuhlangabezana nezidingo eziqinile ezisethwe yiziphathimandla ezilawulayo. Ukuzwela kwayo kwenza ukutholwa kokugxila kwe-DNA kuphansi njengama-picographs ayi-10, kuqinisekiswe ukuthi ukungcoliswa okuncane kukhonjwa futhi kubhekwane nakho ngokushesha.
Izakhi zekhithi yokutholwa kwe-bluekit
● Ukulungele - Ku - Sebenzisa Izinzuzo Zefomethi
I-Bluekit Detection Kit ihlinzekwa ngomumo - Kuleli fomethi Sebenzisa, yenza lula ukuhamba komsebenzi kwamafektri asalela ama-DNA nama-laboratoria. Le fomethi inciphisa isikhathi sokulungiselela futhi yehlisa amandla ephutha lomuntu, ukuthuthukisa ukusebenza kahle kanye nokwethembeka. Ukufakwa kwe-Pre - ama-reagents aqinisekisiwe aqinisekisa imiphumela engaguquki futhi esebenza kabusha kuwo wonke ama-batches ahlukene.
● Ukufakwa kwejika elijwayelekile lokunemba
Isici esiphawuleka sekhithi yokutholwa kwe-Bluekit ukufakwa kwayo kwejika elijwayelekile, elibalulekile ngokugxila kwe-DNA ngokunemba. I-Cardve Ejwayelekile i-Akhawunti yokuhlukahluka kwinqubo ye-PCR, inikeze inkomba ethembekile yemiphumela yokutolika. Lokhu kuqinisekisa ukuthi abakhiqizi abasele be-DNA bangahlola ngokuzethemba ngezinga lokungcoliswa kwemikhiqizo yabo.
Iphrothokholi ye-QPCR eyenziwe kahle kanye nezinhlelo zokusebenza
● Ucwaningo olunzulu nentuthuko
Amaphrothokholi we-Bluekit Detection Kit angumphumela wocwaningo olunzulu kanye nentuthuko, enzelwe ukuhlangabezana nezidingo ezahlukahlukene zabahlinzeki be-DNA abasele kanye nezicelo zokwelashwa kweselula. Lezi protocol ezenziwe kahle zithuthukisa ukutholwa kwe-E.Coli DNA izinsalela, ukuqinisekisa ukuthi abakhiqizi banganamathela ezindinganisweni zokulawula futhi bavikele ukuphepha kweziguli ngempumelelo.
● Ukuguquguquka kwezingqinamba ezinhlotsheni ezahlukahlukene zesampula
Ukuguquguquka kwe-KIT kuvumela ukusetshenziswa kwayo ezinhlobonhlobo eziningi zezinhlobo zesampula, kusuka kumatikuleshini eziyinkimbinkimbi eziwubukiso amasampula ahlanzwa. Lokhu kuguquguquka kubaluleke kakhulu kubakhiqizi abasele be-DNA abasebenza ngemikhiqizo ehlukahlukene, ukuqinisekisa ukuthi amakhono okuthola awakhawulelwe ukwakheka kwesampula.
Ukuzwela nokutholwa kwekhithi
● Ukuthola inani lemizuzu ye-DNA
Ikhithi lokutholwa kwe-Bluekit linikeza ukuzwela okuhlukile, okukwazi ukuthola inani lemizuzu ye-DNA kumasampula ayinkimbinkimbi. Lokhu kuzwakala kuqinisekisa ukuthi ngisho nakumanani emali enqobana ye-E.Coli akhonjwe, okwenza abakhiqizi bakwazi ukugcina amazinga aphezulu okumsulwa komkhiqizo nokuphepha.
● Ukuhlangana namazinga aqinile alawulayo
Ukuhambisana namazinga okulawulwa kwamazwe omhlaba kubaluleke kakhulu kubahlinzeki be-DNA abasele, abakhiqizi, kanye namafekthri. Amandla we-Bluekit Detection Kit ukuthola izinsalela ze-DNA emazingeni okuhlangana noma ezidlula lezi zikhundla zezikhundla ukuzulazula ngokuqiniseka ukucwaningwa kwamabhuku okulawulwa kanye nokuhlola.
Ukuhanjiswa komsebenzi okuqondisiwe kanye nokunciphisa iphutha
● Ukunciphisa amandla ephutha lomuntu
I-Bluekit Detection Kit yenza lula inqubo yokutholwa nomsebenzisi wayo - Idizayini enobungane, inciphisa amandla ephutha lomuntu. Izinqubo ezenzakalelayo kanye nemiyalo ecacile abaqondisi abaqondisi abaqondisi ngesinyathelo ngasinye, eqinisekisa imiphumela engaguquguquki futhi enembile. Le nzuzo yokusebenza kokuhamba kwezithelo zezinzuzo ze-DNA Izinsalela zokusalela izinsalela ngokuthuthukisa ukusebenza kahle kanye nokunciphisa ingozi yedatha eyiphutha.
● Ukusebenza kahle ekusebenzeni kwemiphumela
Ukusebenza kahle emiphumeleni yokucubungula kuyisici esibucayi sabahlinzeki be-DNA abasele okuhlose ukufeza izikhathi zokukhiqiza eziqinile. Ukucutshungulwa kwe-Bluekit Detective Kit okusheshayo nokuthembekile imiphumela kunciphisa isikhathi sokuphumula futhi kusheshise isinqumo - ukwenza izinqubo, ukuvumela abakhiqizi ukuthi balethe imikhiqizo ephephile emakethe ngokushesha.
Isikhathi esiguqukayo sesikhathi kanye nezinzuzo zemakethe
● Ukuthuthukisa izinqubo zokukhiqiza
Isikhathi esiguqukayo esisheshayo esinikelwe yi-Bluekit Detection Kit ukuthuthukisa izinqubo zokukhiqiza, okuvumela abakhiqizi abasele be-DNA ukukhomba nokubhekana nezinkinga zokungcola ngokushesha. Le ndlela esebenzayo inciphisa ukubambezeleka kokukhiqiza futhi iqinisekise ukuhambisana okuqhubekayo namazinga okuphepha.
● Isikhathi esisheshayo - ukumaketha kwemikhiqizo
Kwabahlinzeki abasele be-DNA kanye nabakhiqizi bezokwelapha izingcingo, ukunciphisa isikhathi - ukuletha - inzuzo enkulu yokuncintisana. Ukusebenza kahle kwe-Bluekit Detection Kit kusekela ukukhishwa komkhiqizo okusheshayo, okuvumayo kwezinkampani ukuthi zihlangabezane nesidingo esikhulayo sezindlela ezintsha zokwelapha futhi zithole i-feasonto emakethe ngokushesha.
Ukuhlola kanye nokuqinisekiswa kwezakhi zekhithi
● Ukuhlolwa kokusebenza okuqinile
Izingxenye zekhithi yokutholwa kwe-bluekit zifinyelela ekuhlolweni kokusebenza okuqinile ukuze kuqinisekiswe ukuthembeka nokuvumelana. Le nqubo yokuqinisekisa ngokuphelele iqinisekisa ukuthi ikhithi ngayinye ihlangabezana namazinga aphezulu kakhulu wekhwalithi, ukuhlinzeka amafektri asalela ama-DNA anethuluzi lokungcola ukutholwa.
● Ukuqinisekisa ukuthembeka okuphezulu nokuvumelana
Ukuthembela nokuvumelana kubaluleke kakhulu kubahlinzeki be-DNA abasele abancike kwikhithi yokuthola i-Bluekit yokulawula ikhwalithi. Ngokugcina izindlela eziqinile zokuqinisekisa ikhwalithi, ikhithi ihambisa ukusebenza okungaguquki kuyo yonke imijikelezo yokukhiqiza, ukuqinisa ukuzethemba kubakhiqizi kanye nemizimba yokulawula ngokufanayo.
Isiphetho nobufakazi bomsebenzisi
● Ukwethembana nokwaneliseka phakathi kwabasebenzisi
I-Bluekit Detection Kit ithole ukwethembana nokwaneliseka phakathi kwabasebenzisi bayo, kufakazelwa ngobufakazi obuhle abavela kubakhiqizi abasele be-DNA nabaphakeli. Ukwethembeka kwalo, ukunemba, nokusebenziseka kalula kwenza kube yikhethelo elithandwayo lokugcina ukuphepha komkhiqizo nekhwalithi.
● Ukuqinisekisa ukuphepha komkhiqizo kanye namazinga wekhwalithi
Njengoba izidingo zokwelapha eziphephile nezisebenzayo ziqhubeka zikhuphuka, indima ye-Advanced Det Technologies efana ne-Bluekit Detection Kit iya ngokuya ibalulekile. Ngokuqinisekisa ukuphepha komkhiqizo kanye nokuhambisana namazinga alawulayo, ikhithi isekela abahlinzeki abasele be-DNA kanye nabakhiqizi ekuletheni okuphezulu - Ikhwalithi, imikhiqizo ephephile emakethe.
Mayelana ne-bluekit
I-Bluekit ingumkhiqizo ovelele ngaphansi kukaJiangsu Hillgene, oyibhekele eSuzhou, nge-GMP - izitshalo eziqinisekisiwe kanye nesikhungo se-R & D. IHilllgene isebenzisa ukukhiqiza amasayithi ezweni lonke futhi iyanda emhlabeni jikelele ngesiza eNorth Carolina. Ngokukhethekile ekwelashweni kwamaselula, iHillgene ithuthukisa amapulatifomu e-nucleic acid acid, i-serum - amasiko wamahhala, kanye nobuchwepheshe bokuhlola i-QC. I-Bluekit imele ikhwalithi yokulawula ikhwalithi, isekela ukuthuthukiswa kwemoto - t, tcr - t, kanye nokwelashwa kwamaseli, ukuqhubekisela phambili imikhiqizo yeHllgene ukuletha imikhiqizo yokwelapha ukumaketha ngokushesha futhi inzuzo ezigulini emhlabeni wonke.
Isikhathi Seposi: 2025 - 02 - 19 10 5:54:04
