Ukuqalisa
Insimu yokwelashwa kweseli ibone intuthuko emangalisayo kule minyaka eyishumi edlule, ikakhulukazi ekwandisweni nasekusetshenzisweni kwamaseli wemvelo wokubulala (NK). Lawa maseli omzimba, adlala indima ebalulekile ezinhlwini zokuvikela umzimba, asephenduke angcono ekwakheni ukwelashwa komdlavuza kanye nezinye izifo. Le ndatshana idlulela ezinhlotsheni ze-NK cell Exponsion Kits, indima ye-trophoblast kanye nezobunjiniyela amaseli we-K562, ukubaluleka kwama-cytokines, kanye nezinkomba zesikhathi esizayo ze-NK cell theretres. Ngaphezu kwalokho, sithinta ukubaluleka kweI-Host Cell DNA Kit Peprocessings ekuqinisekiseni ikhwalithi nokuphepha kwemikhiqizo yokwelashwa kwamaseli.
Sibutsetelo Sokunwebeka Kwe-NK cell Exponsion
● Ukubaluleka Ekwesandleni Kwesikweletu
I-NK Cell Expansion Kits ingamathuluzi abucayi emkhakheni wezokwelapha amaseli, okwenza amandla okukhuliswa kwama-nk cell kumazinga wokwelapha. Lawa ma-Kits enza lula ukukhiqizwa kwenani elanele lamaseli we-NK adingekayo kwizicelo zemitholampilo, ngaleyo ndlela athuthukise amandla abo okulashwa.
● Izakhi zekhithi
Imvamisa, amakhithi wokunwetshwa kweseli we-NK aqukethe ama-reagents afaka amaseli okuphakelayo, ama-cytokines, kanye ne-nk cell ekhethekile ye-basal medium. Lezi zingxenye zisebenza ngokuvumelanayo ukuze zidale indawo efanelekile ye-NK cell ukwanda kwesakhiwo kanye nokwenza kusebenze, kuqinisekiswe isivuno esikhulu kanye nobumsulwa.
Indima yamaseli weTrophoblast ku-NK activation
● Indlela yokuvuselela
Amaseli weTrophoblast ngokwemvelo axhumana namaseli we-NK ngesikhathi sokukhulelwa, edlala indima ebalulekile ekuguqukeni umsebenzi wawo. Ngokwesimo sokwandiswa kweseli kwe-NK, amaseli weTrophoblast angavuselela amaseli we-NK ngokusebenzisa i-receptor - Ukusebenzisana kwe-Ligand, ukuthuthukisa umsebenzi wabo we-cytotoxic nokwanda.
● Izinzuzo zokusebenzisa amaseli weTrophoblast
Ukusebenzisa amaseli weTrophoblast ku-NK cell Expansion kuhlinzeka ngendlela yokuthobela eyengeziwe yokuvuselela amaseli we-NK, okuholela kumaseli anokusebenza okuphezulu ngokuqhathaniswa nokunwetshwa ngezindlela zokufakelwa.
I-Engineered K562 amaseli e-NK cell Expansention
● Inkulumo yama-cytokine kumaseli we-K562
Amaseli we-Engineered K562 asetshenziswa kaningi njengamaseli we-feeder ekwandisweni kweseli ye-NK. Zishintshwa ukuveza ama-cytokines anjenge-IL - 15 no-Il - 21, okubaluleke kakhulu ukukhula kweseli ye-NK nokwenza kusebenze.
● Umthelela we-irradiation kanye nokungasebenzi
I-irradiation noma ukungasebenzi kwamaseli we-K562 ngaphambi kokusebenzisa kwabo njengoba amangqamuzana abondli aqinisekisa ukuphepha ngokuvimbela ukwanda okungafuneki, ngenkathi namanje ukugcina amandla abo okusekela ukwanda kweseli ye-NK.
Ukubaluleka kwama-cytokines ku-NK kusebenze
● Il - 21 nemiphumela yayo
Ama-Cytokines anjenge-IL - 21 ahlanganiswe ku-NK Cell activation. Il - 21 akugcini nje kuphela ukukhuthaza ukwanda kweseli ye-nk kepha futhi kuthuthukisa imisebenzi yabo ye-cytotoxic, okwenza kube yingxenye ebalulekile yama-NK cell ukunwebeka.
● I-Synergistic ukusayina izindlela
I-Cytokines isebenza ngokusebenzisa izindlela ezahlukahlukene zokusayina ukuze isebenze amaseli we-NK. Ukuqonda lezi zindlela kubalulekile ukwenza kahle amaphrothokholi we-NK cell ukwelula kanye nokwenza ngcono ukusebenza kwawo kwezokwelapha.
Imithombo yeseli ye-NK
● Ukuqhathanisa isivuno seseli
I-Umbilical Cord Blood ngumthombo ocebile wamaseli we-NK, uvame ukuveza amanani aphezulu uma kuqhathaniswa negazi elihlanganayo. Kodwa-ke, ukukhetha phakathi kwale mithombo kuncike kwizidingo ezithile zohlelo lokusebenza lokwelapha.
● Ubumsulwa kanye nekhwalithi yamangqamuzana anwetshiwe we-NK
Yomibili imithombo inganikeza okuphezulu - amaseli we-NK wekhwalithi, kepha amaphrothokholi wokunwebeka angahluka. Ukuqinisekisa ubumsulwa nokusebenza kwamaseli anwebekayo we-NK kubaluleke kakhulu ngemiphumela ephumelelayo emtholampilo.
Isicelo eCar - NK cell ukulungiselela
● Izinqubo zokuthola imoto - Amaseli we-NK
Imoto - Amaseli we-NK azokwenziwa e-Express Chimeric Antigen Receptors, ukubenza bakwazi ukuqondisa amaseli athile umdlavuza. Ukulungiswa kwemoto - amaseli we-nk kubandakanya ukuguqulwa kofuzo nokwanda, izinqubo ezivuselelwa amakhithi akhethekile.
● Izinzuzo ngaphezu kwezindlela zendabuko
I-Car - Amaseli eNk anikela ngezinzuzo eziningana ngaphezu kwezindlela zendabuko, kufaka phakathi ingozi encishisiwe yokuxhunyelelwa - Ukuqhathanisa - Isifo sokusingathwa
Ukuthuthukiswa kwenqubo yokwelashwa kweseli
● Isikhathi kanye nokusebenza kwezindleko entuthukweni yokuqala
Ukuthuthukisa izinqubo ezisebenzayo zokwelashwa kwe-NK cell kubalulekile ukunciphisa isikhathi nezindleko ezigabeni zokuthuthuka kwangaphambili. Ukwenza kahle ama-protocols kanye nokuvunyelwa okulungele - uku- - ukusebenzisa amakhithi wokunwebisa angahambisa kahle le nqubo.
● Amasu wokwenza kahle imikhiqizo yokwelashwa kwamaseli
Amasu anjengokuzenzakalela, ukucubungula uhlelo oluvaliwe, kanye nokusetshenziswa kwama-microbial - ama-reagents wamahhala abalulekile ekwenzeni ikhwalithi kanye nokuvumelana kwemikhiqizo yokwelashwa kwamaselula.
Ukuthuthukisa ubumsulwa nokuvunyelwa kwamaseli we-NK
● Amasu okuthola ubumsulwa obuphezulu
Amasu anjenge-Flow Cytometry - Ukuhlunga okususelwa kususelwa kanye nokwehlukaniswa kobude bezinkabi kuyaqashwa ukufeza amazinga aphezulu wokuhlanzeka emaseli anwebekayo we-NK, okubaluleke kakhulu kwizicelo zemitholampilo.
● Izinselelo nezixazululo ezikukhulu - Ukunwetshwa kwesilinganiso
Ukulinganisa ukunwetshwa kwe-NK cell kubeka izinselelo ezifana nokugcina ukusebenza kweseli nokusebenza. Imiklamo emisha ye-bioreactor nezinhlelo zokuqapha eziqhubekayo yizisombululo zalezi zinselelo.
Izinzuzo zokusebenzisa i-nk cell basal medium
● Indima ekwandisweni kweseli kanye nokwenza kusebenze
I-NK cell basal medium medium ihlelwe ngokuqondile ukusekela ukukhula kanye nokwenza kusebenze amaseli we-nk, ukuqinisekisa ukusebenza okungaguquki ku-extransion ehlukile yokwandisa.
● Ukuhambisana nama-cytokines ahlukahlukene
Ukuhambisana okuphakathi nohlu lwama-cytokines kuvumela ukuguquguquka ekushintsheni kwe-tailaring ekwindla ye-NK cell ukuhlangabezana nezidingo ezithile zokwelapha.
Izikhombisi zekusasa zokwelapha kwe-NK cell
● Amathrendi avelayo ekwelashweni kweseli
Inkambu ye-NK cell therapy yavela ngokushesha, ngezindlela ezivelayo ezigxile ekuqinisekiseni ukucaciswa, ukunciphisa - imiphumela yokuhlobisa, kanye nokwenza ngcono ukwelashwa kwezindlela zokwelapha.
● Intuthuko engaba khona kuzinhlelo zokusebenza zeselula ze-NK
Intuthuko yesikhathi esizayo ingafaka ukuthuthukiswa kwamaseli we-donror nk wendawo yonke, izindlela zokuhlanganisa namanye amaseli omzimba, kanye nokwelapha okwenziwe ngezifiso kwe-NK.
Ukuqinisekisa ikhwalithi nge-Host Cell DNA PreProcessing Kits
● Ukubaluleka Ekwesandleni Kwesikweletu
I-Host Cell DNA Kits Esetshenziselwa ukwelashwa okwenziwa ngaphambi kokwelashwa kwamaseli ukuthola nokuthola i-DNA ye-Sound Cell. Lawa makhithi aqinisekisa ukuphepha nokusebenza kahle kwemikhiqizo yokwelashwa kwamaselula ngokunciphisa ubungozi bokungcola.
● Umsebenzi wabakhiqizi nabathengisi
Abakhiqizi nabaphakeli be-Host Cell DNA Kits Esedlule, njengeI-Bluekit, dlala indima ebalulekile ekuhlinzekeni ngokwethembekile nangophakeme - amakhithi wekhwalithi. Lawa makhithi abalulekile ukuze alondoloze ubuqotho bezinqubo zokukhiqiza zokwelashwa kwamaselula.
Ukugcina
Isimo sokwelashwa kweseli sivela njalo, ngamaseli we-nk angaphambili walesi singuquko. Ukuhlanganiswa kwama-Kits athuthukile okunwebeka, ama-Cytokine Insighting, kanye namathuluzi wokuqinisekisa ikhwalithi efana ne-Host Cell DNA PreProcessing Kits eshayela ukuthuthukiswa kwezindlela zokwelapha eziphephile nezisebenzayo. Njengoba imboni iqhubekela phambili, ukusebenzisana phakathi kwabaphenyi, odokotela, kanye nabakhiqizi kuzoba ngokhiye ukuvula amandla aphelele wezokwelapha ze-NK.
UJiangsu Hillgene, ngaphansi komkhiqizo wawo weBluekit®, umi phambili we-Cellular Therapy Innovation. Ngendlunkulu yayo eSuzhou nasekukhiqizeni amasayithi eChina naseNyakatho Carolina, iHillgene ingamapulatifomu okuphayona e-nucleic acid ukukhiqiza kanye ne-cell therapy Product Development. Uzibophezele ekulawulweni kwekhwalithi, imikhiqizo ye-BlueKit® iqinisekisa ukuphepha kanye nokusebenza kahle kwezindlela zokwelapha amaseli, ukusekela abalingani bomhlaba wonke ekuzuzeni imoto ephumelelayo - t, tcr - t, iseli elisekelwe. Ngombono wokugqugquzela iselula yokwelashwa okusha, iHillgene inikezelwe ekuthuthukiseni izixazululo zokwelapha zamaselula emhlabeni jikelele.
Isikhathi Sokuposa: 2024 - 12 - 13 15:31:09
