I-Pichia Pastotis hcp (i-protein yeselfowuni) i-Supection Ikhithi
I-Pichia Pastotis hcp (i-protein yeselfowuni) i-Supection Ikhithi
✓ I-PIII Pastoris - I-- Akukho nqamlezweni - Ukusebenza kwakhona nge-cho / hcps
✓ Ilungile - I-- Sebenzisa - Akukho sampula efunekayo
Ukusetyenziswa kwenjongo
Le khithi yenzelwe ukubongwa ubungakanani beeseli zeseli zeseli zeseli (i-HCP) iintsalela kwiimveliso ze-biophamarmaceutical zivela I-Pichia Pagis Imingxunya (umzekelo. Ukuphanda kusetyenziswa kuphela. Hayi ngeenjongo zokuxilonga.
Umgaqo wokufunyanwa
Ikhithi isebenzisa a isandwich Elisa Indlela:
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Ukuthuthumela: I-MicroPist Weds i-pre - idityaniswe ngeegusha ze-polyclon ye-polycals chars I-Pichia Pagis I-HCPS.
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Igalelo: Iisampulu / oose calibrars bongezwa, kulandelwa yi-HRP - I-Polyclon PolyClonswal Anti -I-Pichia Pagis I-HCP Antibodies.
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Ukuqaphela: Emva kokuhlamba, i-TMB yongezwa kuphuhliso lwemibala. Ukuphendula kuyamiswa, kwaye i-ansing ilinganiswa nge I-450 NM.
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UbungakananiI-HCP ye-HCP ibalwa ukusuka kwi-curve esemgangathweni (i-abtorbence α hcp windorition).
Ezona mpawu
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Akukho sampula efunekayo .
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Iprotocol elungelelanisiweyo: Amanyathelo amancinci, iziphumo ezikhawulezayo (
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Ubuchule obuphezulu kunye nokuthembeka: Ulungile I-Pichia Pagis I-HCPS.
Amanqaku ochwephesha
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Iisampulu ezihambelanayo: Inkcubeko ye-Supersuatants, i-biologrics ecocekileyo.
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Uluhlu lwe-Dynamic: I-1-100 NG / ml (eqhelekileyo).
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Ulungelelwaniso olulawulo: Ilungele ukuphuculwa kwenkqubo kunye novavanyo lwe-GMP ye-GMP.
Inkqubo yokusetyenziswa1. Ulungelelaniso
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Zisa onke amacandelo kubushushu begumbi (¶20 Min).
I-2. Isampulu / i-antidy yongeza-
Yongeza iisampulu / ii-calibrators: I-100 μl / kakuhle
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Yongeza i-1x enzyme - i-antickited anti: I-100 μl / Kulungile (jonga kwi-legend ①)
I-3. I-Incubation-
I-Bumate kwi-RT, ikhuselwe ukukhanya, i-600 rpm ye-3 h (Jonga i-legend ③)
4. Ukuhlamba-
Hlamba 1: I-300 μl / i-bill ye-Buffer (indawo ethe nkqo)
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Hlamba 2 - 5: 300 μl / i-bill ye-Buffer, i-5 iyonke
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Pat yomile: Emva kokuhlamba okokugqibela, i-inver kwiplate kwiPhepha leFir
I-5. Uphuhliso lwe-Turst-
Yongeza i-timb: I-100 μl / kakuhle
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Phuhlisa: I-10 Min nge-RT (ikhuselwe ukukhanya)
6. Yima impendulo kunye nokufunda-
Yongeza isisombululo sokumisa: 50 μl / kakuhle
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Yima: 5 imiz
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Funda: I-OD nge-450 NM (isalathiso 620 NM)
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| Icandelo | Imveliso No. | Ukucaciswa | Inkcazo |
|---|---|---|---|
| Ngaphambi kwexesha - I-Anti - I-PICIA HCPP MPAPT | I-PNA015 | I-8 quis × 12 imigca | Ngaphambi kokuba idityaniswe nge-upolyclon anti -I-Pichia Pagis I-HCP Antibodies. Itywinwe kwi-foil pouches enethemba. Ivenkile ekhuselweyo. |
| I-pichia hcp calibrator | Pnb015 | Ii-vials ezi-2 | I-lyophilid powder. Ukufumana kwakhona nge-500 μl calibrator diacent (Pnc002). Vumela i-5 ukuya kwi-10 imiz ukuze ichithelwe (isisombululo kufuneka sicocethile). Ivenkile ekhuselweyo. |
| I-calibtor divakaent | I-Pnc002 | I-1.5 ml × 1 ityhubhu | Isisombululo esicacileyo sokuhlaziya i-pichia hcp calibrator. |
| I-Assay Dundent | Pne006 | I-25 ml × 2 iibhotile | Ukutyibilika kweekhalikisi, iisampulu, kunye ne-enzyme - i-anticted anti. Qinisekisa into ebonakalayo yeesampulu ezintsha. |
| Hlamba i-buffer (10 × joence) | Pnf001 | I-25 ml × 2 iibhotile | Kwiplate yokuhlamba. Ikristali inokwenziwa ngamaqondo obushushu asezantsi - anyibilikiswe kwi-37 ° C ngaphambi kokusetyenziswa. Dilute 10 × ngamanzi okugqibela. |
| I-HRP - I-Anti ye-Anti - I-PICIAN HCP Anti (100 ×) | I-PNN009 | I-120 μl × 1 ityhubhu | I-PolyClontol Anti I-dilute 100 × ne-assay dieucent ngaphambi kokusetyenziswa. Ivenkile ekhuselweyo. |
| I-tictast | I-PN004 | I-12 ml × 1 ibhotile | Ukulingana kubushushu begumbi (≥20 min) ngaphambi kokusetyenziswa. Ivenkile ekhuselweyo. |
| Yeka isisombululo | Pni002 | I-6 ml × 1 ibhotile | Ine-HCL. Nxiba i-goggles kwaye uphephe ukunxibelelana ngolusu. |
| Ukutywina kweefilimu | Pnk001 | Iishiti ezi-3 | Ukugubungela iziqwengana ze-microplate ukuthintela ungcoliseko kunye nokuphuma kwamanzi. |
Ulwazi lokuhambisa
Sinikezela ngothutho oluhlaziyiweyo kuyo yonke imiyalelo. Ngokwesiqhelo, iodolo yakho iya kufika ngaphakathi kwe-5 - Iintsuku ezisi-7 zeshishini eUnited States kwaye kwiintsuku ezili-10 zomsebenzi wamazinye amazwe. Nangona kunjalo, nceda uqaphele ukuba ukuhanjiswa kweendawo ezisemaphandleni kunokuthatha ixesha elide.
Ixesha lokuhambisa: Ii-odolo zihlala zisetyenzwa ngaphakathi 1 - iintsuku ezintathu zomsebenzi. Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile yokuqinisekisa ngolwazi lokulandela umkhondo.
Ulwazi olubalulekileyo
Ukuqhubekeka kocwangco: Emva kokuba i-odolo ihlawulwe, i-Warehouse yethu idinga ixesha lokuqhuba iodolo yakho. Uya kufumana isaziso emva kokuba i-odolo yakho ithunyelwe.
Amaxesha okuhambisa: Kwiimeko ezininzi, iphakheji iya kusiwa ngexesha eliqikelelweyo lokufika. Nangona kunjalo, umhla wokwenyani unokuchaphazeleka ngamalungiselelo eenqwelomoya, iimeko zemozulu, kunye nezinye izinto zangaphandle. Ixesha lokuhanjiswa kwexesha liya kuba ngaphezulu kwesiqhelo semiyalelo ebandakanya ukhuseleko okanye izinto ezenziwe ngokwezifiso. Nceda ujonge ulwazi lokulandelela ngowona mhla uchanekileyo wokuhanjiswa.
Imicimbi yokuhambisa: Ukuba ufumanisa ukuba iphakheji yakho ayihanjiswanga ngexesha elimiselweyo; Ulwazi lokulandela umkhondo lubonisa ukuba iphakheji ihanjisiwe kodwa awukayifumani; Okanye iphakheji yakho ibandakanya izinto ezilahlekileyo okanye ezingachanekanga okanye eminye imiba yempahla, nceda uqhagamshelane nenkonzo yethu yabathengi kwiintsuku ezisi-7 zomhla wokuhlawula ukuze sikwazi ukujongana nale micimbi ngokukhawuleza.
Ukulandelela umkhondo
Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile ngenombolo yokulandela umkhondo kunye nekhonkco lokulandelela ukuthunyelwa kwakho.
Unokulandelela i-odolo yakho ngqo kwiwebhusayithi yethu ngokungena kwiakhawunti yakho kwaye ujonge imbali yakho ye-oda.
Izithintelo zokuhambisa
Nceda ugcwalise idilesi yesitrato ngokweenkcukacha, hayi ibhokisi ye-PO okanye idilesi yomkhosi (AP). Ngaphandle koko, kuya kufuneka sisebenzise i-EMS yokuhanjiswa (ihamba kancinci kunabanye, ukuthatha malunga 1 - iiyure ezi-2 okanye ixesha elide).
Imisebenzi yesiko kunye nomgaqo-nkqubo werhafu
Nceda uqaphele ukuba nayiphi na imisebenzi yesiko, irhafu, okanye intlawulo yokungenisa okwenziweyo ngexesha lokuhambisa luxanduva lomthengi. Ezi ntlawulo ziyahluka ngokuxhomekeke kwilizwe eliya kulo kwaye zimiselwa ngabasemagunyeni basekhaya.
Ngokuthenga kwiwebhusayithi yethu, uyavuma ukuhlawula nayiphi na imisebenzi esebenzayo okanye irhafu enxulumene ne-oda yakho. Asinaxanduva lokulibaziseka okubangelwa kukucocwa kwesiko.
Umgaqo-nkqubo wePakeji yephakheji
Nje ukuba i-odolo yakho ifikile kwindawo echongiweyo yePickup okanye indawo yokuHanjiswa, nceda uqinisekise ukuqokelelwa ngokukhawuleza. Ukuba iphakheji ayithanga ngexesha elichongiweyo, siya kuthumela isikhumbuzo nge-imeyile okanye nge-SMS. Nangona kunjalo, ukuba iphakheji ayiqokelelwa ngaphakathi kwexesha elichaziweyo, kwaye nayiphi na ilahleko okanye umonakalo ovela ngenxa yoko, umthengi uya kuba noxanduva. Ngobubele kukhumbuza ukuba uqokelele iphakheji yakho ngokukhawuleza ukuphepha nayiphi na imiba enokubakho.
Qaphela: Njengoko imveliso yethu iwela phantsi kodidi olukhethekileyo, ibuyise kunye nokubuyiselwa kwemali ayamkelwa.
