I-Cytotocity Cytotocity Assay yeKit
I-Cytotocity Cytotocity Assay yeKit
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✔ I-CYOLS - I-LIlebheli ye-cytotocity icwecwe (CFse + 7 - I-AAD)
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✔Phing
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Uqhekezwa ngemoto - t / nki iiseli zeseli
Amagqabantshintshi:
I UKUSETYENZISWA KWEXESHA LOKUGQIBELA I-Cytotocity Caretocity As Kit yenzelwe ukulinganisa iseli ye-Ecephector - I-Cytotocity yaseCytotocity ngokumiswa - Ibala lemihlaba ekujoliswe kuyo. Ikhithi iqulethe:
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Cfse (I-carboxyfuorescein succifinidyl)
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I-7 - i-aad .
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Inkcubeko ephakathi a &I-Assay buffer
Izicelo
✔ Umsebenzi oSebenzayo wokuvavanywa kweeseli zonyangoIiseli zeNK, Imoto - Nk, Imoto - T, TCR - T, T, I-TM) Ngokuchasene nemigca yokumiswa kwe-tumoor
✔ Inkqubo yokuphuhliswa kwenkqubo: Ukuphucula i-Caldy yeWelkuyili
✔ Ulawulo lwemeko: Uvavanyo lokukhutshwa kunye nezifundo zekliniki - Iimveliso zenqanaba
Ezona mpawu
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I-Dial - ilayidi: I-CFSE (iithagethi eziphilayo) + 7 - I-AAD (iiseli ezifileyo) ze-cytotocity ichanekile
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I-GMP - iyahambelana: Iqinisekisiwe kuphuhliso lonyango lweseli phantsi kwe-FDA / IZIKHOKELO ZE-EMA
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Imsebenzi ye-US: Ngaphambi - Iiprotocol ezifanelekileyo zokudibanisa umthungo
Amagqabantshintshi ezoBugcisa
▸ Ithagethi iguquguqukayo: Ihambelana ne-Tumor eminye yemigca (E.G., K562, Raji)
▸ Ulungelelwaniso olulawulo: Ixhasa i-Ind - ukuvumisa izifundo kunye neephakeji zamaxwebhu
▸ Ukuzala kwakhona: ≤10% cv kwi-PSIT
Ishiti yedatha:
Ukuhamba kwe-cytometry ye-cytometry ye-NK seli - I-Cytotocity Cytotocity kwi-CFSE - IIMFAZWE ZE-K562 Iiseli ekujoliswe kuzo
I-NK eselfowuni i-cytotocity yokubulala ijika kwi-CFSE - IIMFAZWE ZE-K562 Iiseli ekujoliswe kuzo
| Isazisi sekhathalogu | Icandelo | Ubungakanani | Iimeko zokugcina | Indawo yeKit |
|---|---|---|---|---|
| HG - MD001 | Iphakathi a | I-100 ml | 2 - I-8 ° C | Kit i |
| HG - FW001 | I-Assay buffer | I-100 ml | 2 - I-8 ° C | - |
| Hg - cfse | I-cfse stain | I-1 vial | - 20 ° C | Ikhithi II |
| HG - 7 - I-AAD | I-7 - I-Aad Stain | I-1 vial | - 20 ° C | - |
Ukuphononongwa
| Ipharamitha yokusebenza | I-Wally |
| Ukuchaneka | Ukubuyiswa kwakhona 70 - I-130% |
| Ukomelela | I-Bias 70 - I-130% |
| Ukuphindaphinda | I-CV ≤ I-10% |
| Ukuchaneka kokuPhakathi | I-CV ≤ I-10% |
Ulwazi lokuhambisa
Sinikezela ngothutho oluhlaziyiweyo kuyo yonke imiyalelo. Ngokwesiqhelo, iodolo yakho iya kufika ngaphakathi kwe-5 - Iintsuku ezisi-7 zeshishini eUnited States kwaye kwiintsuku ezili-10 zomsebenzi wamazinye amazwe. Nangona kunjalo, nceda uqaphele ukuba ukuhanjiswa kweendawo ezisemaphandleni kunokuthatha ixesha elide.
Ixesha lokuhambisa: Ii-odolo zihlala zisetyenzwa ngaphakathi 1 - iintsuku ezintathu zomsebenzi. Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile yokuqinisekisa ngolwazi lokulandela umkhondo.
Ulwazi olubalulekileyo
Ukuqhubekeka kocwangco: Emva kokuba i-odolo ihlawulwe, i-Warehouse yethu idinga ixesha lokuqhuba iodolo yakho. Uya kufumana isaziso emva kokuba i-odolo yakho ithunyelwe.
Amaxesha okuhambisa: Kwiimeko ezininzi, iphakheji iya kusiwa ngexesha eliqikelelweyo lokufika. Nangona kunjalo, umhla wokwenyani unokuchaphazeleka ngamalungiselelo eenqwelomoya, iimeko zemozulu, kunye nezinye izinto zangaphandle. Ixesha lokuhanjiswa kwexesha liya kuba ngaphezulu kwesiqhelo semiyalelo ebandakanya ukhuseleko okanye izinto ezenziwe ngokwezifiso. Nceda ujonge ulwazi lokulandelela ngowona mhla uchanekileyo wokuhanjiswa.
Imicimbi yokuhambisa: Ukuba ufumanisa ukuba iphakheji yakho ayihanjiswanga ngexesha elimiselweyo; Ulwazi lokulandela umkhondo lubonisa ukuba iphakheji ihanjisiwe kodwa awukayifumani; Okanye iphakheji yakho ibandakanya izinto ezilahlekileyo okanye ezingachanekanga okanye eminye imiba yempahla, nceda uqhagamshelane nenkonzo yethu yabathengi kwiintsuku ezisi-7 zomhla wokuhlawula ukuze sikwazi ukujongana nale micimbi ngokukhawuleza.
Ukulandelela umkhondo
Nje ukuba i-odolo yakho ithunyelwe, uyakufumana i-imeyile ngenombolo yokulandela umkhondo kunye nekhonkco lokulandelela ukuthunyelwa kwakho.
Unokulandelela i-odolo yakho ngqo kwiwebhusayithi yethu ngokungena kwiakhawunti yakho kwaye ujonge imbali yakho ye-oda.
Izithintelo zokuhambisa
Nceda ugcwalise idilesi yesitrato ngokweenkcukacha, hayi ibhokisi ye-PO okanye idilesi yomkhosi (AP). Ngaphandle koko, kuya kufuneka sisebenzise i-EMS yokuhanjiswa (ihamba kancinci kunabanye, ukuthatha malunga 1 - iiyure ezi-2 okanye ixesha elide).
Imisebenzi yesiko kunye nomgaqo-nkqubo werhafu
Nceda uqaphele ukuba nayiphi na imisebenzi yesiko, irhafu, okanye intlawulo yokungenisa okwenziweyo ngexesha lokuhambisa luxanduva lomthengi. Ezi ntlawulo ziyahluka ngokuxhomekeke kwilizwe eliya kulo kwaye zimiselwa ngabasemagunyeni basekhaya.
Ngokuthenga kwiwebhusayithi yethu, uyavuma ukuhlawula nayiphi na imisebenzi esebenzayo okanye irhafu enxulumene ne-oda yakho. Asinaxanduva lokulibaziseka okubangelwa kukucocwa kwesiko.
Umgaqo-nkqubo wePakeji yephakheji
Nje ukuba i-odolo yakho ifikile kwindawo echongiweyo yePickup okanye indawo yokuHanjiswa, nceda uqinisekise ukuqokelelwa ngokukhawuleza. Ukuba iphakheji ayithanga ngexesha elichongiweyo, siya kuthumela isikhumbuzo nge-imeyile okanye nge-SMS. Nangona kunjalo, ukuba iphakheji ayiqokelelwa ngaphakathi kwexesha elichaziweyo, kwaye nayiphi na ilahleko okanye umonakalo ovela ngenxa yoko, umthengi uya kuba noxanduva. Ngobubele kukhumbuza ukuba uqokelele iphakheji yakho ngokukhawuleza ukuphepha nayiphi na imiba enokubakho.
Qaphela: Njengoko imveliso yethu iwela phantsi kodidi olukhethekileyo, ibuyise kunye nokubuyiselwa kwemali ayamkelwa.
