Bubuphi ubushushu obulungele umsebenzi we-Benzozulu?

AmagqabantshintshiI-benzoaseUkusebenza kwe-enzymatic

I-BenzoSele yi-enzyme eguquguqukayo edlala indima ebalulekileyo kwicandelo le-biotechnologiz, ngakumbi ekususweni kweesampulu zenyukliya ezivela kwiisampulu zebhayoloji. Njenge - i-endonkese eveleI-Serratia Marcescens, I-benzoniase icoca zombini iDNA kunye ne-RNA Molecules. Olu phawu luyenza ixabiseke kwizicelo ezahlukeneyo, njengokucoca iiproteni zokugcina izinto kunye nokuqinisekisa ukuthembeka kweenkqubo zokuveliswa kogonyo. Abavelisi kunye nababoneleli beeRectamical ye-biochemical bahlala begxininisa ukusebenza kwe-Benziase kwii-acid ze-acid i-acid ngaphandle kwe-intshi

Indlela yesenzo

I-BenzoOse izenzo ze-hydrolyz i-phoste ye-phoster i-phocren kwi-nucleic acids, ivelisa 5 'i-phosphate - ephelisile i-oligomuecides. I-enzyme isebenza kakhulu ngokuchasene ne-START - ichithwa kwaye iphindwe kabini - I-acid eqingqiweyo, nokuba isetyhula, isetyhula, isetyhula, okanye isukiweyo. Oku kukwazi ukunciphisa ungcoliseko lwe-nucleic ibaluleke kakhulu kwiinkqubo apho intsalela ye-nucleid acid inokuphazamisa izicelo ezisezantsi.

Ukusetyenziswa kwe-BenzoOse kwi-biotechnology

Kumzi-mveliso we-biotechnology, i-BenzoAse iqeshelwe kakhulu ubuchule bayo be-nucleid. Izicelo zayo zihlala imimandla emininzi, kubandakanya ukuveliswa kogonyo, apho ukususwa kweeseli ze-acids nucleic ibalulekile ekukhuseleni nasekusebenziseni imveliso yokugqibela.

Indima kwimveliso yogonyo

Umzi mveliso wamayeza uxhomekeke kwi-Benzoase kubuchule bayo bokunciphisa ukungcola kwe-nucleic kwinqanaba lePicogram. Oku kuqinisekisa ukuba izitofu zokugonya kunye nezinye iiprotheyini - Ukuseka iindlela zokuziphatha gcina amanqanaba aphezulu ococeko. Abavelisi babeke ibeka phambili i-Benzoase kwiiproputho zemveliso zabo ukuze bahlangane nemigangatho yolawulo.

Ukuphuculwa kweenkqubo ezifihlakeleyo

I-BenzoOse ayiyophuliyo kuphela ukuphucula ukususwa kwe-acid ye-sucleid kodwa nakwi-AIDS ekunciphiseni i-viscofity yeysys yeseli. Olu kuncitshiswa kwi-viscofity lukhuthaza ukuqina okukuko ngakumbi kunye neenkqubo zokuphumelela, ezikhokelela kwisivuno esiphuculweyo nobunyulu beemveliso zebhayoloji. Ababoneleli bezixhobo zokugcina izilwanyana zihlala zincoma i-BenzoAse yokuphucula ukulungiswa kwendlela yokucoca.

Ukuqonda ubushushu obufanelekileyo bomsebenzi we-Benzoniase

Iqondo lokushisa lidlala indima ebalulekileyo ekusebenzeni nasezizinzeni ze-enzymes, kubandakanya i-Benzoase. Ukuchonga iqondo lobushushu elifanelekileyo lomsebenzi wayo kunokuphembelela kakhulu ukusebenza kweenkqubo eziqeshiweyo kuzo.

I-enzyme kinetics kunye neqondo lobushushu

I-enzyme kinetics inobuthathaka kumaqondo obushushu ahluka, kunye nomsebenzi oqhele ukwanda kwamaqondo obushushu anyukayo de kufikelwe i-piak. Ngaphandle kwale qondo lobushushu, umsebenzi we-enzyme ngokukhawuleza uyala ngenxa yokutshixisa. I-BenzoOse, iqondo lobushushu eliqinisekileyo lihlala lithathwa njengendawo malunga ne-37 degrees Celsius, ehambelana nemeko yemzimba.

Iqondo lokushisa - Umsebenzi oxhomekekileyo we-Benzoase

Umsebenzi we-BenzoOse kuphenjelelwa ikakhulu liqondo lokushisa, echaphazela inqanaba lokuthandeka kunye nozinzo. Izifundo zibonisa ukuba kumaqondo obushushu asezantsi, umsebenzi we-enzyme uncitshisiwe, ngelixa amaqondo obushushu aphezulu, umngcipheko wokukhwela ukufundwa.

Ubungqina bovavanyo

Kwi-Laboratory useto, i-Benzoonale ibonakalisa umsebenzi omkhulu malunga ne-37 degrees Celsius. Eli qondo lobushushu livumela ukucocwa kwe-nucleid esebenzayo ngelixa ugcina uzinzo lwe-enzyme. Iifektri ezikhethekileyo kwimveliso ye-enzyme zihlala zichaza iinkqubo zazo zokuxhasa eli qondo, ukuqinisekisa ukusebenza okungaguqukiyo kwe-enzyme.

Indima yeqondo lokushisa kwi-enzyme

Ngelixa amaqondo obushushu afanelekileyo aphucula umsebenzi we-enzyme, anokuba nefuthe kubume be-inhibitors. Ukugcina iqondo lobushushu eliphezulu kubalulekile ekunciphiseni iziphumo zothintelo ezinokubangela ukuba kubekho umsebenzi we-Benzoonamase.

Imvakalelo yovelwano

Ubukho be-regents ezithile zinokuthintela umsebenzi we-benzonase ukuya kuthi ga kwi-50% ngokuxinana okuthile. Nangona kunjalo, ukugcina i-enzyme kwiqondo lokushisa eliphezulu linokunciphisa ezi ziphumo, kuqinisekisa ukusebenza ngokuthembekileyo kwizicelo zemveliso.

Ukuqwalaselwa okusebenzayo kulawulo lobushushu kwiinkqubo zemizi-mveliso

Ulawulo lobushushu lubalulekile ekukhuliseni ukusebenza ngempumelelo kwe-Benzoase kwimveliso yeziza-nkqubo. Bobabini abavelisi kunye nabathengisi kufuneka baphumeze iinkqubo zebhotile eziqinisekisa iimeko zobushushu eziguqukayo.

Ukubeka iliso kubushushu kunye nohlengahlengiso

Ukubeka esweni rhoqo kweqondo lobushushu, kudityaniswa neenkqubo zokulungelelanisa amaqondo obushushu ezizenzekelayo, kunokunceda ukugcina umsebenzi ofanelekileyo we-enzyme. Iifektri zihlala zityala imali kubuchwephesha boLawulo lobushushu obuPhakamileyo ukuqinisekisa ukuba iinkqubo zazo zemveliso zihlala zisebenza ngokufanelekileyo kwaye zixabisa iindleko.

Ukuziqhelanisa nokuzimisela kweqondo lobushushu

Ukuchonga i-Optima ye-Optima ye-Benzonase ibandakanya uvavanyo olucwangcisiweyo, ukuvumela ukulingana kwezicelo zeshishini. Oku kuqinisekisa ukuba amandla e-enzyme adityaniswe ngokupheleleyo kwiinkqubo ezahlukeneyo zebhayoloji.

Iindlela zokuvavanywa

Iiprotokholi ezisemgangathweni ezisemgangathweni zibandakanya ukuvavanya umsebenzi we-Benzonase kuluhlu lwamaqondo obushushu. Abavelisi baqhele ukuqhuba ezi mvavanyo ngexesha leSigaba sokuPhuhliswa kwe-Enzyme ukuseka ezona meko zingasekhoyo zobushushu zokuhanjiswa kwezicelo ezahlukeneyo.

Ukuthelekisa i-Banzoase kunye nezinye ii-endowoses

I-Bensonase imele phakathi kwe-endony Cedoseases ngenxa ye-sando ebanzi - Umsebenzi wobushushu kunye nokuzimisela ubushushu, kokwenza ukhetho olukhethwayo phakathi kwabaxhasi bezisombululo ze-enzymatic. Izifundo zokuthelekisa zinceda ezigxininisa ezi zibonelelo kwizicelo zawo ezahlukeneyo.

Izibonelelo zokusetyenziswa kwezinye i-enzymes

Xa kuthelekiswa nezinye ii-endowoases, i-BenzoOse ibonelela nge-acid ephezulu ye-acid kulo lonke uluhlu lobushushu. Oku kusebenza kubalulekile kubavelisi bafune izisombululo ezinokuthenjwa nezifanelekileyo ze-Encyminitic zoMngeni oQeqeshayo bokugcina izinto ezinzima zokugcina izinto ezinzima.

Izikhombisi zexesha elizayo kwimikhombandlela yobushushu be-enzyme

Uphando oluqhubekayo luyaqhubeka ukuphucula ukuqonda kwethu ukuqonda kweqondo lokushisa i-enzyme, ngenjongo yokuphucula ukusebenza kwe-Benzoonaase kunye nozinzo. Olu phando lubalulekile ekugcineni umda we-enzyme kwicandelo lokhuphiswano lwe-biloveroces.

Izinto ezintsha kwi-enzyme yobunjineli

Ukuhambela phambili kubunjineli beProtein kunokukhokelela kuphuhliso lwe-BenzoOse Drisoants kunye nozinzo lobushushu obuphuculweyo kunye nomsebenzi. Ngokugxila kwezi mveliso, abathengisi kunye nabathengisi banokubonelela ngezisombululo ezisebenzayo ezisebenzayo ezilungiselelwe iimfuno ezithile zeshishini.

I-Bluekit ibonelela ngezisombululo

I-Bluekit ibonelela ngezisombululo ezijolise ekwenzeni umsebenzi we-Benzonase kwiimeko ezahlukeneyo zobushushu. Iinkqubo zethu zokuphila ze-biprocececececececececececececececes eziphezulu ze-biprocececececececececececececececececececececes tyala ziqinisekisa ulawulo lobushushu oluchanekileyo, ukuphucula ukusebenza kakuhle kunye nokuzinza. Ngokudibanisa imeko - Ubungcali bethu kwiZisombululo ze-Enzymatic zikuxhobisela ngezixhobo ezifunekayo ukulungisa imiceli mngeni enzima yokufundisa, ukuqinisekisa ukusebenza kakuhle kwe-enzyme kunye nococeko lwemveliso.