Nguwuphi umahluko phakathi kwe-trpsin kunye nezinye iiProCEAseS?

Intshayelelo kwiiProteeks kwiiNkqubo zeBiologional

Iiproteks, iqela elihlukeneyo le-enzymes, zibaluleke kwiinkqubo ezininzi zebhayiloji, ukusuka ekugayeni ukuya kwiseli. Basebenza ngokuthatha i-cleaving icocravage yebhondi yepeptide kwiiproteni, ngaloo ndlela idlala indima ebalulekileyo ekugcineni isemachi yeselula. Iiproteks zihlelwe ngokusekwe kwiindlela zabo zengozi kunye neendlela ezibonisa ubukhulu becala, nge-serieaseases ye-Serneaseases njenge-trypsin ukuba phakathi kweyona nto ifundwayo. Eli nqaku lihlola iimpawu ezikhethekileyo ze-trypsin ngokwahlukileyo kwezinye iiprosesa, ziqwalasele iinkalo ezifana nolwakhiwo, ukusebenza kunye nokusetyenziswa.

Trypsin: Umgangatho wegolide kwiProteyisi

Indima ye-trypsin kwi-matchrometry

Trypsin leads the proteomics field due to its high specificity and efficiency in cleaving peptide chains at the carboxyl side of lysine and arginine residues. Olu bheko lwezinto ezinokubangela iziqwengana kunye nentlawulo efanelekileyo kwi-C - I-terminus, lungelelanisa uhlalutyo lwe-spectrometry (MS). Kwi-Proprosics, amandla e-trypsin ukuvelisa isikhonkwane sobude obufanelekileyo aphucula ukuchongwa kweproteni, inkxalabo ebalulekileyo yophando lweseli kuphando lweseli, apho kufuneka ubonakalise uphawu lweprotein.

Izibonelelo kwinkcazo yeprotein

I-proteomics ixhomekeke kwi-trypries eyahlukileyo ye-trypsin yesazisi seprotein. Izifundo zibonisa ukuba itrapsin ingachonga iiproteni ezingaphezulu kwe-80% ngaphezulu xa kuthelekiswa neeprosesa ezinomxholo obanzi, owenza ukuba ingabandakanyeki kubaphandi kunye nabathengisi ebaleni. Indima yayo isuka ekuqondeni iindlela zesifo ekuqhubeleni phambili iindlela zonyango, ezinje ngonyango lweseli.

Iimpawu zesakhiwo se-trypsin

Indawo esebenzayo kunye nenkqubo yekatala

Ukusebenza kakuhle kwe-trypsin stems ukusuka kwi-Wells yayo - Ingxelo esebenzayo, ebonisa i-Triely Triad ye-Serine, ihilidine, kunye ne-ASTATATE. Olu hlengahlengiso luququzelela ukucoceka kwe-peptide ye-peptide, imfuneko ebaluleke kakhulu kubathengisi ababonelela ngee-enzymes ezihlanjwe nezicelo zophando. Ubungqola be-enzyme bubakho kubukho bokuhlawuliswa kakubi indawo yokuhlala, etsala i-lysine entle kunye ne-prigine.

I-STOFIMS ye-trypsin

I-Trypsin ikhona kwii-i-itsoms ezininzi, kubandakanya i-cudici, ananguc, kunye ne-mesotrypsinogen. Inxalenye nganye isebenza kwindima eyahlukileyo, kunye ne-cuyasic trypsin ukuba yeyona ibalulekileyo kwipancreas yomntu. I-Mesotrypsin iyakhawuleza ngenxa yokumelana nayo kwi-inhibitors, idlale indima ekhethekileyo ekunciphiseni i-inhibitor - iiproteri ezityebileyo. Ukuqonda ezi ndawo kubalulekile kwiifektri ezichaphazelekayo kwimveliso ye-enzyme yokusetyenziswa konyango.

Olunye uCempleiases kunye neeNgcaciso

Uthelekisa i-aspn kunye ne-gluc

I-ASPN kunye ne-GLuc ekujoliswe kuyo i-Amino ye-Amino Amino, inika idatha ehambelana nayo kwi-trypsin. Ezi proteases zivelisa umxube we-peptide oqingqiweyo ezifanelekileyo kwiimfuno ezithile zohlalutyo kwiProprosics. Nangona kunjalo, ubuso babo ngokubanzi buhlala bubangelwa kukuncitshiswa kweprotein yeprotein xa kuthelekiswa ne-trypsin.

I-chymotrypyopsin kunye neeproteatoions ngokubanzi

I-chymotrypsin yahlukile kwi-trypsin ngokujolisa kwi-hydrophobic enkulu ye-hydrophobic, njenge-tryptophan kunye ne-tyrosine. Ngelixa ibambe imali ebaluleke kakhulu, ukusetyenziswa kwayo kudla ngokulinganiselwa kwizicelo ezithile. Bubanzi - IPROATION COPEAPSE K Prothiinase K Veentances entsonkothileyo yePeptede yePeptdede, echaza imiceli mngeni yokufumana i-protiemics, ngaphandle kokuba isetyenziswa ngokudibeneyo ne-trypsin.

Iindlela zokuhamba ngokulandelelana nge-trypsin

Ukuchongwa kweprotein ephuculweyo

Ukuhamba ngokulandelelana, kubandakanya ukusetyenziswa kwe-trypsin elandelwa ngolunye uKhosa, ukuphucula ubuqhetseba. Umzekelo, ukuqhubela phambili nge-trypsin kunokunyusa izichazi zeprotheni zeprotheyini k nge-731%. Kwizicelo zonyango lweseli, eli mbali livumela ukuba uqiniseke ngakumbi profayili, ibaluleke kakhulu ekuqondeni ngeendlela zeselula.

Ukukhuselwa kwe-peptides ngokulandelelana

I-petidedies encinci eveliswe yi-trypsin ikhuselwe ukusuka kumgangatho ophezulu kwiinkqubo ezilandelelanayo, ezikhokelela ekubuyiseleni okuncinci kunokuba kuchaziwe kwi-Silico uhlalutyo. Le ndlela iyingenelo kubaxhasi ababonelela ngeenkonzo zeprojetho, njengoko ziqinisekisa ukuchaneka okuphezulu kwi-Iteyisi yeProtein kunye nophawu.

Ukuchasana nokujongana ne-IPROORE

Iiprosesa ezinobunye obukhulu, njenge-trypsin, zithandwa ngenxa yokwazi kwabo ukuvelisa iziqwenga ze-peptide nezilawulwayo. Ngokwahlukileyo, iiproteases ngokubanzi zikhokelela kwimixube entsonkothileyo, ukutolikwa idatha engcwekisayo. Iifektri ezivelisa i-enzymes yophando kufuneka ziqwalasele lo mahluko wokufezekisa iimfuno zezinto ezithile zeprotecotic kakhulu kwizicelo ezahlukeneyo.

Indima ye-zymogens kwi-Expersise

I-Protease Appersor isebenze

Iiprosesa zihlala zihambelana njenge-zymogening ze-zymogening ukunqanda ukutshatyalaliswa okungafunekiyo kwiiproteni zeselula. I-Trypsinogen, ivuliwe kumathumbu amancinci, ibonisa kakuhle le nto. Ukuqonda i-zeymogen ukusebenza kubalulekile kwiifektri ezivelisa iiproteni zokusebenzisa ezonyango, ekuqinisekiseni ukuba i-enzymes esebenzayo inikezelwa kwiimeko ezilawulwayo.

Ummiselo nge-Enterokinase

I-Enterokinase idlala indima ebalulekileyo ekusebenzeni kwe-trypsinogen, ukuqinisa ukubaluleka kommiselo ochanekileyo kwi-Protease. Ibhalansi phakathi kwe-Zymogesi kunye nokuSebenza kuqinisekisa ukuba umsebenzi weeproteolytic wenzeka ngendlela efanelekileyo emzimbeni, into ebaluleke kakhulu kubaxhasi abasebenza kwiinkqubo zokusiwa kwe-enzyme.

IProtease inhibitors kunye nemisebenzi yazo yebhayiloji

Ulawulo loMsebenzi weProteolytic

I-Intersease inhibitors zibalulekile ekulawuleni komsebenzi weproteolytic kwiinkqubo zebhayiloji. Banqanda ukupheliswa okungalawulekiyo kweeproteni, ibhalansi ebalulekileyo kwizinto eziphezulu. Kwiseli yonyango, ukuqonda le mali kubalulekile ekuphuhliseni unyango olusebenzisa umsebenzi wokuthintela umsebenzi ngokufanelekileyo.

IPROSTEAKIA UKUNYANGELWA KWAKHONA

Iiproteksi zothintelo azinezindiso kuphela kwimigaqo yemveli kodwa nakwizicelo zonyango. Banika izibonelelo ezinokubakho, njengokuthintela ukonakala kwezintso kwimingcipheko ye-antibiotics okanye ukunciphisa umngcipheko weTumorgenesis, ebonisa ukubaluleka kwabo kunyango nophuhliso.

Ukusetyenziswa kwemizi-mveliso kunye ne-bietechnoin ye-trypsin

Indima kwi-biotechnology

I-trypsin isetyenziswa ngokubanzi kwiinkqubo ze-bietechnological, ezinjengeeproteni zeproteni kunye nokusasaza iseli. Ubungqina bayo kunye nokusebenza kakuhle kwenza ukuba kukhetho olukhethwayo kwizicelo ezininzi, kubandakanya neefektri ezivelisa iimveliso zonyango zeseli, apho kulungelelwaniso lomsebenzi we-enzymatic kubalulekile.

I-Bluekit ibonelela ngezisombululo

I-Bluekit ibonelela ngezisombululo ezibanzi zilungelelaniswe ukuba zifezekise iimfuno zeprotease zophando kunye nezicelo zemveliso. Ngokubonelela nge-trippin ephezulu kunye nezinye iiprosesa, iBluekit iqinisekisa ukuba iilebhu kunye neefektri zinezixhobo eziyimfuneko ukuqhubela phambili umsebenzi wabo kwi-protetipy, kunye ne-biotechnology. Ukuzibophelela kwethu kwimeko esemgangathweni kunye nokwenza isikhundla esihle njengomthengisi ophambili, ukukunceda ufezekise iziphumo ezinokuthenjwa kwaye zichanekile kwimizamo yakho yenzululwazi.