Intshayelelo yovavanyo lwe-DNA
Uvavanyo lwe-DNA lwe-DNA lubhekisa kwiindlela zohlalutyo ezisetyenziselwa ukufumana kwaye ziqwalasele umkhondo we-DNA yeDNA ehlala kwiimveliso ze-byphaphasmarmacmarmical emva kokwenza iinkqubo zemveliso. Olu hlobo lovavanyo lubalulekile ekuqinisekiseni ukhuseleko nokucoceka kweebhayiloji, kubandakanya unyango, izitofu zokugonya, kunye nee-antiboutic. Ubukho beDNA yentsalela kwi-biophasmarmaceuticals, ngakumbi i-DNA isuka kwiiseli ezigciniweyo njenge-e.coli, ibeka umngcipheko onokubakho kwi-immunonisity kunye ne-tumsubinicity. Ke ngoko, uvavanyo lwe-DNA olujikelezayo lwe-DNA luyinxalenye ebalulekileyo yolawulo oluphambili kwimveliso ye-biophaphargucal.
● Inkcazo kunye nokubaluleka
Ukuvavanywa kwe-DNA Ezi qhekeza zinokwahluka ngokobukhulu kunye nobungakanani, kunye neemali zomzuzu zinokubaluleka. Ukubaluleka kovavanyo lwe-DNA yentsalela akunakuba ngaphezulu-iqinisekisa ukuba i-biophasmarmicaluticals ihlangana nemigangatho yolawulo yokhuseleko kunye nokusebenza ngokusebenza kakuhle, ngaloo ndlela ukukhusela impilo yesigulana.
● Sebenzisa kulawulo oluphambili
Ulawulo olusemgangathweni kwimveliso ye-biophaphacceutical lubandakanya amanqanaba amaninzi, ukusuka ekuqinisekisweni kwezinto ezikrwada kuvavanyo lwemveliso yokugqibela. Uvavanyo lwe-DNA lwe-DNA lubaluleke kakhulu ngaphakathi kwesi sikhokelo. Iqinisekisa ukuba iinkqubo zokucamngca zisuse ngempumelelo izinto zemvelo, ukuqinisekisa ukuthotyelwa kwemveliso yokugqibela yezikhokelo ezibekwe ngabasemagunyeni abalawulayo njenge-FDA kunye ne-EMA.
Indima yeDNA yentsalela kwi-biophapharmaceuticals
● Iindidi zee-biophapharmaceuticals
I-Biopharmaoruticals ibandakanya uluhlu olubanzi lweemveliso, kubandakanya i-antibodies ye-monoclon, iiproteni zokulungisa, ugonyo, kunye nonyango lweseli. Udidi ngalunye luneenkqubo ezizodwa zemveliso, kodwa zonke zichaphazeleka ukuba i-DNA yokungatshiyisi.
● Imithombo yeDNA yentsalela
I-DNA ye-DNA ikakhulu ivela kwiiseli ezamkelayo ezisetyenziswa kwinkqubo yemveliso. Iiseli eziqhelekileyo zemakhosi zibandakanya iiseli zentsholongwane ezinje nge-e.coli, iiseli zegwele, iiseli zeMammalian, kunye neeseli zenambuzane. Ngexesha lokuveliswa kwee-biophapharmaceuticals, ezi senjeni zisenziwa ukuba kuvune imveliso oyifunayo, okunokwenzeka ngokukhulula izinto zabo zemfuza kumxube.
Imigaqo ye-Taqman Probe kwi-DNA yokufunyanwa
● Indlela yokusebenza
I-Taqman Probe - I-Assay esekwe yindlela esetyenzisiweyo esetyenzisiweyo ye-DNA yentsalela ye-DNA. Le ndlela isebenzisa i-fluorencerly ebizwa ngokuba yi-hybridment yokwaziswa kwe-DNA. I-Taq Polymerase e-Enzyme icoca iProbe ngexesha lenkqubo ye-PCR ye-PRRR, eyahlula idayi ye-fluorescent ukusuka kwi-quencher kwaye ivelise uphawu olukhoyo.
● Idini le-Taqman Probe
Enye yezona zibonelelo ziphambili ze-Taqman Probe yinto yawo. Ukwazi kukaProbhe kwi-hybridize kuqinisekisa ukuba kuphela i-DNA ekujoliswe kuyo iphakanyisiwe kwaye ifunyenwe, ukunciphisa ipostatus elungileyo. Le ndlela ikwabonelela ngemvakalelo ephezulu, iyenza ilungele ukufumana amanqanaba asezantsi eDNA yentsalela.
E.coli njengeseli yenginginya kwii-biophapharmaceuticals
● Kutheni le.Coli isetyenziswa rhoqo
E.coli sisisele esithandwayo esithandwayo kwi-biotechnology ngenxa yokukhula ngokukhawuleza, ngokufanelekileyo - kunye nokukwazi ukuveza amanqanaba aphezulu eeproteni eziphindaphindiweyo. Ezi mpawu zenza i-E.Coli indleko - Ukusebenza okusebenzayo kunye nemveliso enkulu - Ukuveliswa kwesikali.
● Iimpembelelo zentsalela ye-E.Coli DNA
Ngaphandle kwezibonelelo zayo, ukusetyenziswa kwe-E.Coli kuza nomngcipheko wokungcoliswa kwe-DNA. Le DNA yentsalela inokubeka inkxalabo yokhuseleko, njengokuthintela ukudluliselwa kwemfuza okanye ubukho be-endotoxin. Ke ngoko, iindlela ze-DNA ze-DNA ze-DNA ze-DNA zibalulekile xa usebenzisa i-E.COLI njengomkhosi wemveliso.
Iindlela zokuchonga ubungakanani
● Iindlela ezisetyenzisiweyo zisetyenzisiwe
Iindlela ezininzi zobuchwephesha ziqeshwa ekungenenisweni kwe-DNA yentsalela, kubandakanya i-QPCR, idijithali pcr, kunye nelandelayo - ukulandelelana kwezizukulwane. Indlela nganye inikezela izibonelelo ezahlukeneyo ngokwemvakalelo, kubunyani, kunye negalelo.
● Imvakalelo kunye nokuchaneka
Kuvavanyo lwe-DNA ye-DNA, ubuntununtunu kunye nokuchanekayo yeyona nto iphambili. Iindlela zobuchule ze-QPCR kunye neDijithali PCR inokubona i-DNA kumanqanaba e-phytogram, ibonelela ngeemvakalelo eziphezulu eziyimfuneko ukuqinisekisa ukhuseleko lwemveliso. Ukuchaneka kubalulekile ngokulinganayo, njengoko kuqinisekisa ukuthembeka kweziphumo zovavanyo, ukuvumela izigqibo eziqinisekileyo - Ukwenza ulawulo oluphambili.
Ukubaluleka kokufunyanwa komgangatho we-FG
● Inkcazo yenqanaba le-FG
Inqanaba le-FG libhekisa kwi-FebtoGograms, iyunithi yemilinganiselo emele i-10 ^ - IIMPAWU ezili-15. Ukufumana i-DNA kwinqanaba le-phytogram libonisa i-assoy ebuthathaka kakhulu ekwaziyo ukuchonga umkhondo wezinto zemvelo.
● Ukubaluleka Imvakalelo ephezulu
Imvakalelo eninzi kuvavanyo lwe-DNA yentsali ibaluleke kakhulu ekuqinisekiseni ukhuseleko lwee-biophapharmaceuticals. Ukufumana i-DNA kwinqanaba le-FG livumela ukuchongwa kweyona nto ibalaseleyo, ukuqinisekisa ukuba imveliso yokugqibela icocekile kangangoko kunokwenzeka kwaye idibane nemigangatho yolawulo.
Amanyathelo olawulo lomgangatho kwimveliso ye-biophapharmication
● Ufuna uvavanyo lwe-DNA
Isidingo sovavanyo lwe-DNA oshiyekileyo kwimveliso ye-biophaphacceutical stems ukusuka kumngcipheko onokubakho onxulumene nongcoliseko lwendalo. Iiarhente ezilawulayo zigunyazisa iindawo ezingqongqo kumanqanaba e-DNA yentsalela, inyanzelisa ngeendlela zokuvavanya ezomeleleyo ukuqinisekisa ukuthotyelwa.
● Imigangatho yolawulo
Imigangatho yolawulo ye-DNA yentsalela iyahluka ngokuxhomekeka kuhlobo lwe-biophaphaphacal. Umzekelo, i-FDA kunye ne-EMA ziseke izikhokelo ezichonge imiqobo eyamkelekileyo yeDNA yentsalela kwiimveliso ezahlukeneyo. Ukubambelela kule migangatho kubalulekile ukuba ivunyenwe imveliso kunye nokukhutshwa kwemarike.
Izicelo kwimveliso yokufumana iprotein
● Izifundo ezithile
Kwimveliso yeproteni iprotein, uvavanyo lwe-DNA lwe-DNA lubalulekile ekuqinisekiseni ubucoceko bemveliso. Izifundo ezithile zibonisa ukusetyenziswa ngempumelelo kweI-E.Coli DNA I-DNA IKITS Ukubeka iliso kunye namanqanaba ongcoliseko lwe-DNN, ukuqinisekisa ukuthotyelwa kwemigangatho yolawulo.
● Ukuqinisekiswa komgangatho
Ukuqinisekiswa komgangatho kwimveliso yokufumana iprotein kubandakanya iintlobo ezininzi zovavanyo kunye nokuqinisekiswa. Uvavanyo lwe-DNA lusebenzisana nendima ephambili kule nkqubo, ukubonelela ngedatha efunekayo ukuqinisekisa ukuba iinkqubo zokucamngca zisuse ngokufanelekileyo.
Imiceli mngeni kuvavanyo lwe-DNA
● Ubunzima bobuchwephesha
Omnye wemiceli mngeni ephambili kuvavanyo lwe-DNA oshiyekileyo bubunzima obuchwephesha bokufumana kunye nokufumana amanqanaba asezantsi eDNA. Izinto ezinjengeMatrix ye-Matrix, ukwahlulwa kwe-DNA, kunye ne-Assay
● Ukoyisa imiqobo eqhelekileyo
Ukoyisa le miqobo kufuna ukusetyenziswa kweendlela eziphambili kunye neeprotokholi ezifanelekileyo. Abavelisi kunye nabathengisi be-E.Coli DNA dna i-kits wits asebenze ngokuqhubekayo ukuze baphumelele iimveliso, ukwandisa ubuntununtunu kunye nokucinga ngokuthi bafezekise iimfuno zeshishini.
Iindlela zexesha elizayo kuvavanyo lwe-DNA
● Inkqubela phambili yetekhnoloji
Inkqubela phambili yetekhnoloji ilungiselelwe ukuguqula uvavanyo lwe-DNA ye-DNA. Izinto ezintsha ezinje ngeCrispr - I-Ascres esekwe, kunye neDijithali PCR, kwaye elandelayo - Ukulandelelana kwezizukulwane zinika iindlela ezintsha ezinobuthathaka kakhulu kunye nokufunyanwa kwe-DNA ebuthathaka kakhulu.
● Iindlela ezivelayo kunye nezixhobo
Iindlela ezivelayo kunye nezixhobo kwisithembiso se-DNA STALS STUST yokuphucula ukuchaneka kunye nokusebenza kakuhle kwamanyathelo olawulo lomgangatho. Olu gonyo luya kwenza ukuba abavelisi be-biopharmacegical bahlangane imigangatho yolawulo eyandayo ngelixa kuqinisekisa ukhuseleko lwemveliso.
Ukuqukumbela
Uvavanyo lwe-DNA lwe-DNA luyicandelo elibalulekileyo lolawulo lomgangatho kwimveliso ye-biophasmarcegical. Ukufunyanwa kunye nokudityaniswa kwexabiso le-DNA kubalulekile ekuqinisekiseni ukhuseleko kunye nokusebenza ngempumelelo kwebhayilojini. Iindlela eziphambili ezifana ne-Taqman Probe zibonelela ngovakalelo kunye nokuqina okufunekayo ukuze kuhlangatyezwane nemigangatho yolawulo. Njengoko ishishini liyaqhubeka nokuvela, izinto ezintsha kuvavanyo lwe-DNA lwe-DNA ziya kudlala indima ebalulekileyo ekugcineni amanqanaba aphezulu omgangatho wemveliso.
● MalungaI-Bluekit
I-jiangsu yaseJiangsu, phantsi kwegama lophawu lwe-Bluekit, wamisela ikomkhulu layo eSuzhou ene-10,000㎡ GMP ye-GEP kunye ne-R & D. Ngeendawo zokuvelisa kwi-Shenzhen, Shanghai, kunye nendawo entsha ekwakhiwe kwi-North Carolina, induli iyandisa ubukho bayo behlabathi. Inkampani ithathe inxaxheba kuphuhliso lweekhitha zolawulo ezisemgangathweni kwiimveliso zonyango zeselula, amaqabane axhasayo kuphuhliso oluphumelelayo lwemoto - T, i-T, i-T, iimveliso ezisekwe. I-Bluekit izibophelele ekuqhubeni i-Innovation yeSeli yoNyango kunye nokubonelela ngezisombululo ezinikezelweyo kwiimveliso zonyango lweselula.
IXESHA LOKUQALA: 2024 - 09 - 23 14:17:04
